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Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

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YFP-immunoreactive innervation of sympathetic preganglionic neurons (SPN). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in horizontal sections through the spinal cord at upper thoracic (Upper T; A and B) and lower thoracic/upper lumbar (Lower T/Upper L; C) levels. At both spinal levels (B and C), black YFP-immunoreactive axons most densely innervate the central autonomic area (CAA), which lies dorsal to the central canal (cc) in lamina X. The intermediolateral cell column (IML) receives a moderately dense black YFP innervation. The lateral funiculus (LF; A and B) contains primarily black YFP-immunoreactive axons of passage that run rostrocaudally and occasional axons that travel mediolaterally (small arrows in A). The neurons indicated by arrows D–F are shown at higher magnification in D–F. Micrographs in montages: A, 3; B, 9; C, 6. (D–F) Black YFP-immunoreactive boutons from close appositions (arrows) on brown ChAT-immunoreactive SPN in the CAA. E, Montage of 2 micrographs. Scale bars: A–C, 100 μm; D–F, 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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f0025: YFP-immunoreactive innervation of sympathetic preganglionic neurons (SPN). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in horizontal sections through the spinal cord at upper thoracic (Upper T; A and B) and lower thoracic/upper lumbar (Lower T/Upper L; C) levels. At both spinal levels (B and C), black YFP-immunoreactive axons most densely innervate the central autonomic area (CAA), which lies dorsal to the central canal (cc) in lamina X. The intermediolateral cell column (IML) receives a moderately dense black YFP innervation. The lateral funiculus (LF; A and B) contains primarily black YFP-immunoreactive axons of passage that run rostrocaudally and occasional axons that travel mediolaterally (small arrows in A). The neurons indicated by arrows D–F are shown at higher magnification in D–F. Micrographs in montages: A, 3; B, 9; C, 6. (D–F) Black YFP-immunoreactive boutons from close appositions (arrows) on brown ChAT-immunoreactive SPN in the CAA. E, Montage of 2 micrographs. Scale bars: A–C, 100 μm; D–F, 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Mentions: In both transverse (Fig. 4) and horizontal (Fig. 5) sections, it was clear that the YFP innervation of the CAA was considerably more dense than the YFP innervation of either the IML or the ICN. There were also YFP-immunoreactive axons in the dorsolateral and lateral funiculi (Figs. 4A–E and 5A, B). Many of the varicose YFP-immunoreactive axons in the IML and ICN in spinal segments T1–L2 closely apposed the cell bodies and proximal dendrites of ChAT-immunoreactive SPN (Fig. 4A, B, E and F). Similarly, varicose YFP-positive axons in dorsal lamina X often formed close appositions on ChAT-positive SPN perikarya in the CAA (Figs. 4G, H and 5D–F). In both the IML and CAA, some ChAT-positive neurons received more than one apposition from YFP-immunoreactive terminals. This was particularly the case for SPN that lay within dense arrays of YFP-positive axons (Fig. 4B, H).


Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

YFP-immunoreactive innervation of sympathetic preganglionic neurons (SPN). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in horizontal sections through the spinal cord at upper thoracic (Upper T; A and B) and lower thoracic/upper lumbar (Lower T/Upper L; C) levels. At both spinal levels (B and C), black YFP-immunoreactive axons most densely innervate the central autonomic area (CAA), which lies dorsal to the central canal (cc) in lamina X. The intermediolateral cell column (IML) receives a moderately dense black YFP innervation. The lateral funiculus (LF; A and B) contains primarily black YFP-immunoreactive axons of passage that run rostrocaudally and occasional axons that travel mediolaterally (small arrows in A). The neurons indicated by arrows D–F are shown at higher magnification in D–F. Micrographs in montages: A, 3; B, 9; C, 6. (D–F) Black YFP-immunoreactive boutons from close appositions (arrows) on brown ChAT-immunoreactive SPN in the CAA. E, Montage of 2 micrographs. Scale bars: A–C, 100 μm; D–F, 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4300405&req=5

f0025: YFP-immunoreactive innervation of sympathetic preganglionic neurons (SPN). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in horizontal sections through the spinal cord at upper thoracic (Upper T; A and B) and lower thoracic/upper lumbar (Lower T/Upper L; C) levels. At both spinal levels (B and C), black YFP-immunoreactive axons most densely innervate the central autonomic area (CAA), which lies dorsal to the central canal (cc) in lamina X. The intermediolateral cell column (IML) receives a moderately dense black YFP innervation. The lateral funiculus (LF; A and B) contains primarily black YFP-immunoreactive axons of passage that run rostrocaudally and occasional axons that travel mediolaterally (small arrows in A). The neurons indicated by arrows D–F are shown at higher magnification in D–F. Micrographs in montages: A, 3; B, 9; C, 6. (D–F) Black YFP-immunoreactive boutons from close appositions (arrows) on brown ChAT-immunoreactive SPN in the CAA. E, Montage of 2 micrographs. Scale bars: A–C, 100 μm; D–F, 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Mentions: In both transverse (Fig. 4) and horizontal (Fig. 5) sections, it was clear that the YFP innervation of the CAA was considerably more dense than the YFP innervation of either the IML or the ICN. There were also YFP-immunoreactive axons in the dorsolateral and lateral funiculi (Figs. 4A–E and 5A, B). Many of the varicose YFP-immunoreactive axons in the IML and ICN in spinal segments T1–L2 closely apposed the cell bodies and proximal dendrites of ChAT-immunoreactive SPN (Fig. 4A, B, E and F). Similarly, varicose YFP-positive axons in dorsal lamina X often formed close appositions on ChAT-positive SPN perikarya in the CAA (Figs. 4G, H and 5D–F). In both the IML and CAA, some ChAT-positive neurons received more than one apposition from YFP-immunoreactive terminals. This was particularly the case for SPN that lay within dense arrays of YFP-positive axons (Fig. 4B, H).

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

Show MeSH
Related in: MedlinePlus