Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.
Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.
Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.Show MeSH
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Mentions: Spinal injections of the retrograde tracer FG in five mice and subsequent immunoperoxidase processing of cords to detect FG-immunoreactivity allowed us to assess whether spinal YFP-expressing neurons in the LE sent axons to the brain (Fig. 3). The FG injection sites (Fig. 3A) were centered on the IML between segments T8 and T10. FG-immunoreactivity spread throughout the spinal gray and white matter for one to two segments in both rostral and caudal directions. To quantify the proportion of spinal YFP-containing neurons that sent axons through the T9 injection sites, we used double immunoperoxidase staining to localize FG (black) and YFP (brown) in transverse sections from two mice (Fig. 3B) and counted neurons that were single-stained for YFP or double-stained for YFP + FG. We examined 55 YFP-immunoreactive neurons in one mouse and 43 in the other mouse. Although neurons retrogradely labeled with FG lay very near to the YFP-expressing neurons (Fig. 3B), not one of the 98 spinal YFP-immunoreactive neurons that we examined showed immunoreactivity for FG.
Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.