Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.
Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.
Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.Show MeSH
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Mentions: Although native YFP fluorescence (Fig. 1) revealed that YFP-expressing neurons existed in the lumbosacral spinal cord, an anti-GFP antibody with peroxidase detection was better for defining the morphology of the spinal YFP-positive neurons (Fig. 2). YFP-immunoreactive cell bodies were present in the deep dorsal horn (laminae IV and V) and were located mainly in the LE. The segmental distribution of YFP-positive spinal neurons never overlapped with the segmental distribution of sympathetic preganglionic neurons (SPN). However, rare YFP-expressing neurons occurred in the same sections as the most rostrally located PPN. The spinal YFP-immunoreactive neurons had fusiform or multipolar cell bodies and a small number of dendrites with few branches. The best-stained YFP-positive neurons had dendrites that extended for long distances both laterally and dorsally. The primary and secondary dendrites of YFP-immunoreactive neurons generally were smooth but a few intensely stained dendrites exhibited small dendritic spines. The somata of a number of the heavily stained YFP-expressing neurons gave rise to very fine processes that traveled for variable distances without changing diameter, a morphology that is suggestive of axons.
Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.