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Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

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YFP-immunoreactive cell bodies and dendrites at the level of the lumbar enlargement (LE). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lower lumbar spinal cord. (A) A YFP-immunoreactive cell body (black) in lamina V of the dorsal horn (DH) at spinal level L4–5. ChAT-immunoreactive somatic motor neurons (brown) occur in lamina IX in the ventral horn (VH). The arrow indicates the location of the neuron shown in B. cc, central canal. Montage of 18 micrographs. Scale bar = 250 μm. (B) Higher magnification image of the black YFP-immunoreactive neuron indicated by B in A. The boxed area is shown at higher magnification in Fig. 9A. Montage of 9 micrographs. (C and D) Other examples of black YFP-immunoreactive cell bodies and their dendrites in lamina V of the lumbar enlargement. (D) Montage of 2 micrographs. Scale bars in B–D, 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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f0010: YFP-immunoreactive cell bodies and dendrites at the level of the lumbar enlargement (LE). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lower lumbar spinal cord. (A) A YFP-immunoreactive cell body (black) in lamina V of the dorsal horn (DH) at spinal level L4–5. ChAT-immunoreactive somatic motor neurons (brown) occur in lamina IX in the ventral horn (VH). The arrow indicates the location of the neuron shown in B. cc, central canal. Montage of 18 micrographs. Scale bar = 250 μm. (B) Higher magnification image of the black YFP-immunoreactive neuron indicated by B in A. The boxed area is shown at higher magnification in Fig. 9A. Montage of 9 micrographs. (C and D) Other examples of black YFP-immunoreactive cell bodies and their dendrites in lamina V of the lumbar enlargement. (D) Montage of 2 micrographs. Scale bars in B–D, 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Mentions: Although native YFP fluorescence (Fig. 1) revealed that YFP-expressing neurons existed in the lumbosacral spinal cord, an anti-GFP antibody with peroxidase detection was better for defining the morphology of the spinal YFP-positive neurons (Fig. 2). YFP-immunoreactive cell bodies were present in the deep dorsal horn (laminae IV and V) and were located mainly in the LE. The segmental distribution of YFP-positive spinal neurons never overlapped with the segmental distribution of sympathetic preganglionic neurons (SPN). However, rare YFP-expressing neurons occurred in the same sections as the most rostrally located PPN. The spinal YFP-immunoreactive neurons had fusiform or multipolar cell bodies and a small number of dendrites with few branches. The best-stained YFP-positive neurons had dendrites that extended for long distances both laterally and dorsally. The primary and secondary dendrites of YFP-immunoreactive neurons generally were smooth but a few intensely stained dendrites exhibited small dendritic spines. The somata of a number of the heavily stained YFP-expressing neurons gave rise to very fine processes that traveled for variable distances without changing diameter, a morphology that is suggestive of axons.


Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

YFP-immunoreactive cell bodies and dendrites at the level of the lumbar enlargement (LE). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lower lumbar spinal cord. (A) A YFP-immunoreactive cell body (black) in lamina V of the dorsal horn (DH) at spinal level L4–5. ChAT-immunoreactive somatic motor neurons (brown) occur in lamina IX in the ventral horn (VH). The arrow indicates the location of the neuron shown in B. cc, central canal. Montage of 18 micrographs. Scale bar = 250 μm. (B) Higher magnification image of the black YFP-immunoreactive neuron indicated by B in A. The boxed area is shown at higher magnification in Fig. 9A. Montage of 9 micrographs. (C and D) Other examples of black YFP-immunoreactive cell bodies and their dendrites in lamina V of the lumbar enlargement. (D) Montage of 2 micrographs. Scale bars in B–D, 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4300405&req=5

f0010: YFP-immunoreactive cell bodies and dendrites at the level of the lumbar enlargement (LE). Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lower lumbar spinal cord. (A) A YFP-immunoreactive cell body (black) in lamina V of the dorsal horn (DH) at spinal level L4–5. ChAT-immunoreactive somatic motor neurons (brown) occur in lamina IX in the ventral horn (VH). The arrow indicates the location of the neuron shown in B. cc, central canal. Montage of 18 micrographs. Scale bar = 250 μm. (B) Higher magnification image of the black YFP-immunoreactive neuron indicated by B in A. The boxed area is shown at higher magnification in Fig. 9A. Montage of 9 micrographs. (C and D) Other examples of black YFP-immunoreactive cell bodies and their dendrites in lamina V of the lumbar enlargement. (D) Montage of 2 micrographs. Scale bars in B–D, 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Mentions: Although native YFP fluorescence (Fig. 1) revealed that YFP-expressing neurons existed in the lumbosacral spinal cord, an anti-GFP antibody with peroxidase detection was better for defining the morphology of the spinal YFP-positive neurons (Fig. 2). YFP-immunoreactive cell bodies were present in the deep dorsal horn (laminae IV and V) and were located mainly in the LE. The segmental distribution of YFP-positive spinal neurons never overlapped with the segmental distribution of sympathetic preganglionic neurons (SPN). However, rare YFP-expressing neurons occurred in the same sections as the most rostrally located PPN. The spinal YFP-immunoreactive neurons had fusiform or multipolar cell bodies and a small number of dendrites with few branches. The best-stained YFP-positive neurons had dendrites that extended for long distances both laterally and dorsally. The primary and secondary dendrites of YFP-immunoreactive neurons generally were smooth but a few intensely stained dendrites exhibited small dendritic spines. The somata of a number of the heavily stained YFP-expressing neurons gave rise to very fine processes that traveled for variable distances without changing diameter, a morphology that is suggestive of axons.

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

Show MeSH
Related in: MedlinePlus