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Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

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Distribution of spinal axons showing native YFP fluorescence in segments L3-S1. (A) Schematic showing the locations of YFP-fluorescent axons and cell bodies in longitudinal sections through the lower lumbar and upper sacral spinal cord at dorsoventral levels indicated in the diagram of the transverse section, which shows L3 (modified from the Allen Brain Institute Reference Atlas; http://mousespinal.brain-map.org/imageseries/showref.html). (B) In the lower lumbar cord, YFP-fluorescent nerve cell bodies (arrows) as well as YFP-fluorescent varicose and non-varicose axons occur within the deep dorsal horn (DH). (C) In dorsal lamina X, YFP-fluorescent axons travel rostrocaudally and mediolaterally. A rostrocaudal tract of varicose YFP-fluorescent axons (stars) lies just dorsal to the central canal. Scale bars: B and C = 100 μm.
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f0005: Distribution of spinal axons showing native YFP fluorescence in segments L3-S1. (A) Schematic showing the locations of YFP-fluorescent axons and cell bodies in longitudinal sections through the lower lumbar and upper sacral spinal cord at dorsoventral levels indicated in the diagram of the transverse section, which shows L3 (modified from the Allen Brain Institute Reference Atlas; http://mousespinal.brain-map.org/imageseries/showref.html). (B) In the lower lumbar cord, YFP-fluorescent nerve cell bodies (arrows) as well as YFP-fluorescent varicose and non-varicose axons occur within the deep dorsal horn (DH). (C) In dorsal lamina X, YFP-fluorescent axons travel rostrocaudally and mediolaterally. A rostrocaudal tract of varicose YFP-fluorescent axons (stars) lies just dorsal to the central canal. Scale bars: B and C = 100 μm.

Mentions: The distribution of glucagon promoter-driven YFP expression in coronal and longitudinal sections of spinal cord between thoracic segment 1 (T1) and sacral segment 4 (S4) was assessed in seven adult YFP-PPG mice. ‘Native’ YFP-fluorescence was strong and both YFP-fluorescent axons and cell bodies could be identified without immunostaining (Fig. 1), as has been observed in brainstem (Hisadome et al., 2010; Llewellyn-Smith et al., 2011).


Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Distribution of spinal axons showing native YFP fluorescence in segments L3-S1. (A) Schematic showing the locations of YFP-fluorescent axons and cell bodies in longitudinal sections through the lower lumbar and upper sacral spinal cord at dorsoventral levels indicated in the diagram of the transverse section, which shows L3 (modified from the Allen Brain Institute Reference Atlas; http://mousespinal.brain-map.org/imageseries/showref.html). (B) In the lower lumbar cord, YFP-fluorescent nerve cell bodies (arrows) as well as YFP-fluorescent varicose and non-varicose axons occur within the deep dorsal horn (DH). (C) In dorsal lamina X, YFP-fluorescent axons travel rostrocaudally and mediolaterally. A rostrocaudal tract of varicose YFP-fluorescent axons (stars) lies just dorsal to the central canal. Scale bars: B and C = 100 μm.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300405&req=5

f0005: Distribution of spinal axons showing native YFP fluorescence in segments L3-S1. (A) Schematic showing the locations of YFP-fluorescent axons and cell bodies in longitudinal sections through the lower lumbar and upper sacral spinal cord at dorsoventral levels indicated in the diagram of the transverse section, which shows L3 (modified from the Allen Brain Institute Reference Atlas; http://mousespinal.brain-map.org/imageseries/showref.html). (B) In the lower lumbar cord, YFP-fluorescent nerve cell bodies (arrows) as well as YFP-fluorescent varicose and non-varicose axons occur within the deep dorsal horn (DH). (C) In dorsal lamina X, YFP-fluorescent axons travel rostrocaudally and mediolaterally. A rostrocaudal tract of varicose YFP-fluorescent axons (stars) lies just dorsal to the central canal. Scale bars: B and C = 100 μm.
Mentions: The distribution of glucagon promoter-driven YFP expression in coronal and longitudinal sections of spinal cord between thoracic segment 1 (T1) and sacral segment 4 (S4) was assessed in seven adult YFP-PPG mice. ‘Native’ YFP-fluorescence was strong and both YFP-fluorescent axons and cell bodies could be identified without immunostaining (Fig. 1), as has been observed in brainstem (Hisadome et al., 2010; Llewellyn-Smith et al., 2011).

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

Show MeSH
Related in: MedlinePlus