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Molecular characterization of the apical organ of the anthozoan Nematostella vectensis.

Sinigaglia C, Busengdal H, Lerner A, Oliveri P, Rentzsch F - Dev. Biol. (2014)

Bottom Line: In bilaterians they are characterised by a tuft of long cilia and receptor cells and they are associated with groups of neurons, but their relatively low morphological complexity and dispersed phylogenetic distribution have left their evolutionary relationship unresolved.To provide a foundation for a better understanding of this structure we have characterised the molecular composition of the apical organ of the sea anemone Nematostella vectensis.Our study provides a molecular characterization of the apical organ of Nematostella and represents an informative tool for future studies addressing the development, function and evolutionary history of apical organ cells.

View Article: PubMed Central - PubMed

Affiliation: Sars Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgt 55, 5008 Bergen, Norway.

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Apical organ genes with additional cell-type specific expression (A) The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx. (B) NvCellulase positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx, scattered ectodermal cells are also present. (C) The uncharacterized gene identified by the ID number 239479 (ao81) is expressed in few ectodermal cells in the aboral half of the larvae. (D) The coiled-coil domain containing gene NvCCDC81 (ao155) is detected in individual cells throughout the entire ectoderm (picture focuses on the surface). The embryos displayed are all at planula stage, the aboral pole is to the left. Scale bar=100 µm.
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f0020: Apical organ genes with additional cell-type specific expression (A) The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx. (B) NvCellulase positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx, scattered ectodermal cells are also present. (C) The uncharacterized gene identified by the ID number 239479 (ao81) is expressed in few ectodermal cells in the aboral half of the larvae. (D) The coiled-coil domain containing gene NvCCDC81 (ao155) is detected in individual cells throughout the entire ectoderm (picture focuses on the surface). The embryos displayed are all at planula stage, the aboral pole is to the left. Scale bar=100 µm.

Mentions: Some of the identified apical organ genes displayed additional expression that appeared to be restricted to particular cell-types. The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx (Fig. 4A). NvCellulase-positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx (Fig. 4B). Compared to the often spindle-shaped NvPOMGnT1-like expressing cells, the NvCellulase-positive cells appear more compact. On the basis of the predicted gene function and the distribution of these cells, we assume that the NvCellulase-positive cells are a particular type of gland cells (gland cells with translucent vesicles (Nakanishi et al., 2012)). One of the uncharacterized apical organ genes (ao81) is expressed in only a few ectodermal cells in the aboral half of the larvae, whereas the coiled-coil domain containing gene NvCCDC81 (ao155) can be detected in individual cells throughout the ectoderm (Fig. 4C and D).


Molecular characterization of the apical organ of the anthozoan Nematostella vectensis.

Sinigaglia C, Busengdal H, Lerner A, Oliveri P, Rentzsch F - Dev. Biol. (2014)

Apical organ genes with additional cell-type specific expression (A) The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx. (B) NvCellulase positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx, scattered ectodermal cells are also present. (C) The uncharacterized gene identified by the ID number 239479 (ao81) is expressed in few ectodermal cells in the aboral half of the larvae. (D) The coiled-coil domain containing gene NvCCDC81 (ao155) is detected in individual cells throughout the entire ectoderm (picture focuses on the surface). The embryos displayed are all at planula stage, the aboral pole is to the left. Scale bar=100 µm.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300403&req=5

f0020: Apical organ genes with additional cell-type specific expression (A) The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx. (B) NvCellulase positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx, scattered ectodermal cells are also present. (C) The uncharacterized gene identified by the ID number 239479 (ao81) is expressed in few ectodermal cells in the aboral half of the larvae. (D) The coiled-coil domain containing gene NvCCDC81 (ao155) is detected in individual cells throughout the entire ectoderm (picture focuses on the surface). The embryos displayed are all at planula stage, the aboral pole is to the left. Scale bar=100 µm.
Mentions: Some of the identified apical organ genes displayed additional expression that appeared to be restricted to particular cell-types. The O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase gene NvPOMGnT1-like (ao51) is expressed in scattered cells in the ectoderm and in the pharynx (Fig. 4A). NvCellulase-positive cells (ao132) are enriched in a broad domain in the aboral ectoderm and in the pharynx (Fig. 4B). Compared to the often spindle-shaped NvPOMGnT1-like expressing cells, the NvCellulase-positive cells appear more compact. On the basis of the predicted gene function and the distribution of these cells, we assume that the NvCellulase-positive cells are a particular type of gland cells (gland cells with translucent vesicles (Nakanishi et al., 2012)). One of the uncharacterized apical organ genes (ao81) is expressed in only a few ectodermal cells in the aboral half of the larvae, whereas the coiled-coil domain containing gene NvCCDC81 (ao155) can be detected in individual cells throughout the ectoderm (Fig. 4C and D).

Bottom Line: In bilaterians they are characterised by a tuft of long cilia and receptor cells and they are associated with groups of neurons, but their relatively low morphological complexity and dispersed phylogenetic distribution have left their evolutionary relationship unresolved.To provide a foundation for a better understanding of this structure we have characterised the molecular composition of the apical organ of the sea anemone Nematostella vectensis.Our study provides a molecular characterization of the apical organ of Nematostella and represents an informative tool for future studies addressing the development, function and evolutionary history of apical organ cells.

View Article: PubMed Central - PubMed

Affiliation: Sars Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgt 55, 5008 Bergen, Norway.

Show MeSH