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Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

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MMP-2 (A and B) and TIMP-1 (C) tissue levels. (A) Total and active (lower bars) MMP-2 contents estimated by gelatin zymography. (B) In the zymogram, MMP-2 standard and pooled tissue extracts (1.0 μl) from 30 individual 8-mm skin explants per group were loaded into each lane. Lane 1, rhMMP-2 (50 pg; PF037); 2, native skin; 3, control; 4, TNF-α. (C) TIMP-1 levels determined by ELISA. (A and C) Pooled tissue extracts from five separate native or organ-cultured 8-mm skin explants from each of the donors and group were used for the analyses. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Mean ± SEM (n = 6).
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fig0045: MMP-2 (A and B) and TIMP-1 (C) tissue levels. (A) Total and active (lower bars) MMP-2 contents estimated by gelatin zymography. (B) In the zymogram, MMP-2 standard and pooled tissue extracts (1.0 μl) from 30 individual 8-mm skin explants per group were loaded into each lane. Lane 1, rhMMP-2 (50 pg; PF037); 2, native skin; 3, control; 4, TNF-α. (C) TIMP-1 levels determined by ELISA. (A and C) Pooled tissue extracts from five separate native or organ-cultured 8-mm skin explants from each of the donors and group were used for the analyses. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Mean ± SEM (n = 6).

Mentions: MMP-2 tissue levels were semiquantified by gelatin zymography (Fig. 8A and B). No significant effects of TNF-α treatment on either total or active MMP-2 were observed.


Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

MMP-2 (A and B) and TIMP-1 (C) tissue levels. (A) Total and active (lower bars) MMP-2 contents estimated by gelatin zymography. (B) In the zymogram, MMP-2 standard and pooled tissue extracts (1.0 μl) from 30 individual 8-mm skin explants per group were loaded into each lane. Lane 1, rhMMP-2 (50 pg; PF037); 2, native skin; 3, control; 4, TNF-α. (C) TIMP-1 levels determined by ELISA. (A and C) Pooled tissue extracts from five separate native or organ-cultured 8-mm skin explants from each of the donors and group were used for the analyses. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Mean ± SEM (n = 6).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300401&req=5

fig0045: MMP-2 (A and B) and TIMP-1 (C) tissue levels. (A) Total and active (lower bars) MMP-2 contents estimated by gelatin zymography. (B) In the zymogram, MMP-2 standard and pooled tissue extracts (1.0 μl) from 30 individual 8-mm skin explants per group were loaded into each lane. Lane 1, rhMMP-2 (50 pg; PF037); 2, native skin; 3, control; 4, TNF-α. (C) TIMP-1 levels determined by ELISA. (A and C) Pooled tissue extracts from five separate native or organ-cultured 8-mm skin explants from each of the donors and group were used for the analyses. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Mean ± SEM (n = 6).
Mentions: MMP-2 tissue levels were semiquantified by gelatin zymography (Fig. 8A and B). No significant effects of TNF-α treatment on either total or active MMP-2 were observed.

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

Show MeSH
Related in: MedlinePlus