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Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

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LDH activity in media from cultured skin explants (A), and in cell lysates of and media from normal human epidermal keratinocytes (B). (A) Individual samples, each comprising the combined media from 5 separate 8-mm skin explants, were analyzed. n = number of skin donors. (B) Confluent keratinocytes of the second passage were treated with or without TNF-α for 2 days. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 versus control.
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fig0020: LDH activity in media from cultured skin explants (A), and in cell lysates of and media from normal human epidermal keratinocytes (B). (A) Individual samples, each comprising the combined media from 5 separate 8-mm skin explants, were analyzed. n = number of skin donors. (B) Confluent keratinocytes of the second passage were treated with or without TNF-α for 2 days. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 versus control.

Mentions: The LDH activity in media of control skin cultures decreased (p < 0.005) from day 4 to day 8. LDH was higher with GM6001 (10 μM) treatment on day 8 and with TNF-α days 4 and 8 compared with control (Fig. 3A). In normal human epidermal keratinocytes, TNF-α treatment increased cellular LDH (Fig. 3B). The total number of cells after 2 days treatment was 4.9 ± 0.4 × 105 in control wells and 5.0 ± 0.8 × 105 in TNF-α wells. The corresponding viabilities were 97.0 ± 0.6% and 96.0 ± 0.6%.


Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

LDH activity in media from cultured skin explants (A), and in cell lysates of and media from normal human epidermal keratinocytes (B). (A) Individual samples, each comprising the combined media from 5 separate 8-mm skin explants, were analyzed. n = number of skin donors. (B) Confluent keratinocytes of the second passage were treated with or without TNF-α for 2 days. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 versus control.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4300401&req=5

fig0020: LDH activity in media from cultured skin explants (A), and in cell lysates of and media from normal human epidermal keratinocytes (B). (A) Individual samples, each comprising the combined media from 5 separate 8-mm skin explants, were analyzed. n = number of skin donors. (B) Confluent keratinocytes of the second passage were treated with or without TNF-α for 2 days. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 versus control.
Mentions: The LDH activity in media of control skin cultures decreased (p < 0.005) from day 4 to day 8. LDH was higher with GM6001 (10 μM) treatment on day 8 and with TNF-α days 4 and 8 compared with control (Fig. 3A). In normal human epidermal keratinocytes, TNF-α treatment increased cellular LDH (Fig. 3B). The total number of cells after 2 days treatment was 4.9 ± 0.4 × 105 in control wells and 5.0 ± 0.8 × 105 in TNF-α wells. The corresponding viabilities were 97.0 ± 0.6% and 96.0 ± 0.6%.

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

Show MeSH
Related in: MedlinePlus