Limits...
Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

Show MeSH

Related in: MedlinePlus

Effect of TNF-α on collagen degradation of cultured human skin explants monitored by hydroxyproline-containing peptides released into the media after 8 days in culture. The accumulated amount of hydroxyproline from 10 separate organ-cultured 8-mm explants of each of the 6 donors and group (control and TNF-α) was used for the global calculations. Mean ± SEM (n = 6). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4300401&req=5

fig0010: Effect of TNF-α on collagen degradation of cultured human skin explants monitored by hydroxyproline-containing peptides released into the media after 8 days in culture. The accumulated amount of hydroxyproline from 10 separate organ-cultured 8-mm explants of each of the 6 donors and group (control and TNF-α) was used for the global calculations. Mean ± SEM (n = 6). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Mentions: We first established the time-dependence on collagen degradation in the skin explants by measuring the release of hydroxyproline into the culture medium. From the initiation of culture to day 4 a total of 1.4 ± 0.4 (n = 6) μg hydroxyproline was released. Then a profound increase (p < 0.005) in hydroxyproline liberation occurred to day 8 amounting to 19.8 ± 10.6 (n = 6) μg. Addition of TNF-α to the medium resulted in 1.8 ± 0.5 (n = 6) μg hydroxyproline release over days 0–4 and 38.7 ± 11.3 (n = 6) μg over days 4–8. Overall, TNF-α treatment increased the cumulative hydroxyproline in the medium over 8 days (40.5 ± 11.7 μg) in all six donors (p < 0.005) compared with control (21.2 ± 11.0 μg) treatment (Fig. 1).


Tumor necrosis factor-α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo.

Ågren MS, Schnabel R, Christensen LH, Mirastschijski U - Eur. J. Cell Biol. (2014)

Effect of TNF-α on collagen degradation of cultured human skin explants monitored by hydroxyproline-containing peptides released into the media after 8 days in culture. The accumulated amount of hydroxyproline from 10 separate organ-cultured 8-mm explants of each of the 6 donors and group (control and TNF-α) was used for the global calculations. Mean ± SEM (n = 6). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300401&req=5

fig0010: Effect of TNF-α on collagen degradation of cultured human skin explants monitored by hydroxyproline-containing peptides released into the media after 8 days in culture. The accumulated amount of hydroxyproline from 10 separate organ-cultured 8-mm explants of each of the 6 donors and group (control and TNF-α) was used for the global calculations. Mean ± SEM (n = 6). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Mentions: We first established the time-dependence on collagen degradation in the skin explants by measuring the release of hydroxyproline into the culture medium. From the initiation of culture to day 4 a total of 1.4 ± 0.4 (n = 6) μg hydroxyproline was released. Then a profound increase (p < 0.005) in hydroxyproline liberation occurred to day 8 amounting to 19.8 ± 10.6 (n = 6) μg. Addition of TNF-α to the medium resulted in 1.8 ± 0.5 (n = 6) μg hydroxyproline release over days 0–4 and 38.7 ± 11.3 (n = 6) μg over days 4–8. Overall, TNF-α treatment increased the cumulative hydroxyproline in the medium over 8 days (40.5 ± 11.7 μg) in all six donors (p < 0.005) compared with control (21.2 ± 11.0 μg) treatment (Fig. 1).

Bottom Line: Levels of the collagenases MMP-8 and MMP-13 were insignificant and neither MMP-2 nor MMP-14 were associated with increased collagen degradation.Type I collagen formation was down-regulated in cultured compared with native skin explants but was not reduced further by TNF-α.TNF-α had no significant effect on epidermal apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: saag0005@regionh.dk.

Show MeSH
Related in: MedlinePlus