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Identification and analysis of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 in cynomolgus macaques.

Uno Y, Hosaka S, Yamazaki H - J. Vet. Med. Sci. (2014)

Bottom Line: By phylogenetic analysis, each cynomolgus P450 was more closely related to the human ortholog as compared with the dog or rat ortholog.Tissue expression patterns of each cynomolgus P450 were generally similar to that of the human ortholog.These results suggest the molecular similarities of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 between cynomolgus macaques and humans.

View Article: PubMed Central - PubMed

Affiliation: Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642-0017, Japan.

ABSTRACT
Cytochromes P450 (P450) are important for not only drug metabolism and toxicity, but also biosynthesis and metabolism of cholesterol and bile acids, and steroid synthesis. In cynomolgus macaques, widely used in biomedical research, we have characterized P450 cDNAs, which were isolated as expressed sequence tags of cynomolgus macaque liver. In this study, cynomolgus CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 cDNAs were characterized by sequence analysis, phylogenetic analysis and tissue expression pattern. By sequence analysis, these five cynomolgus P450s had high sequence identities (94-99%) to the human orthologs in amino acids. By phylogenetic analysis, each cynomolgus P450 was more closely related to the human ortholog as compared with the dog or rat ortholog. By quantitative polymerase chain reaction, among the 10 tissue types, CYP7A1 and CYP17A1 mRNAs were preferentially expressed in liver and adrenal gland, respectively. Cynomolgus CYP27A1 and CYP51A1 mRNAs were most abundantly expressed in liver and testis, respectively. Cynomolgus CYP20A1 mRNA was expressed in all the tissues, including brain and liver. Tissue expression patterns of each cynomolgus P450 were generally similar to that of the human ortholog. These results suggest the molecular similarities of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 between cynomolgus macaques and humans.

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Tissue distribution of cynomolgus P450 mRNA. qPCR was conducted using total RNA ofbrain, lung, heart, liver, kidney, adrenal gland, small intestine, testis, ovary anduterus as described in text. For each tissue, total RNAs from six animals (three malesand three females) were pooled and used for qPCR. Expression level of P450 mRNA wasnormalized to 18S rRNA level. Values represent the average ± S.D. from three independentamplifications. For graphic representation, the highest value of each P450 mRNA wasadjusted to 1.
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fig_002: Tissue distribution of cynomolgus P450 mRNA. qPCR was conducted using total RNA ofbrain, lung, heart, liver, kidney, adrenal gland, small intestine, testis, ovary anduterus as described in text. For each tissue, total RNAs from six animals (three malesand three females) were pooled and used for qPCR. Expression level of P450 mRNA wasnormalized to 18S rRNA level. Values represent the average ± S.D. from three independentamplifications. For graphic representation, the highest value of each P450 mRNA wasadjusted to 1.

Mentions: qPCR analysis showed that among the 10 tissue types analyzed, cynomolgus CYP7A1 and CYP17A1mRNAs were preferentially expressed in liver and adrenal gland, respectively (Fig. 2Fig. 2.


Identification and analysis of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 in cynomolgus macaques.

Uno Y, Hosaka S, Yamazaki H - J. Vet. Med. Sci. (2014)

Tissue distribution of cynomolgus P450 mRNA. qPCR was conducted using total RNA ofbrain, lung, heart, liver, kidney, adrenal gland, small intestine, testis, ovary anduterus as described in text. For each tissue, total RNAs from six animals (three malesand three females) were pooled and used for qPCR. Expression level of P450 mRNA wasnormalized to 18S rRNA level. Values represent the average ± S.D. from three independentamplifications. For graphic representation, the highest value of each P450 mRNA wasadjusted to 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300383&req=5

fig_002: Tissue distribution of cynomolgus P450 mRNA. qPCR was conducted using total RNA ofbrain, lung, heart, liver, kidney, adrenal gland, small intestine, testis, ovary anduterus as described in text. For each tissue, total RNAs from six animals (three malesand three females) were pooled and used for qPCR. Expression level of P450 mRNA wasnormalized to 18S rRNA level. Values represent the average ± S.D. from three independentamplifications. For graphic representation, the highest value of each P450 mRNA wasadjusted to 1.
Mentions: qPCR analysis showed that among the 10 tissue types analyzed, cynomolgus CYP7A1 and CYP17A1mRNAs were preferentially expressed in liver and adrenal gland, respectively (Fig. 2Fig. 2.

Bottom Line: By phylogenetic analysis, each cynomolgus P450 was more closely related to the human ortholog as compared with the dog or rat ortholog.Tissue expression patterns of each cynomolgus P450 were generally similar to that of the human ortholog.These results suggest the molecular similarities of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 between cynomolgus macaques and humans.

View Article: PubMed Central - PubMed

Affiliation: Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642-0017, Japan.

ABSTRACT
Cytochromes P450 (P450) are important for not only drug metabolism and toxicity, but also biosynthesis and metabolism of cholesterol and bile acids, and steroid synthesis. In cynomolgus macaques, widely used in biomedical research, we have characterized P450 cDNAs, which were isolated as expressed sequence tags of cynomolgus macaque liver. In this study, cynomolgus CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 cDNAs were characterized by sequence analysis, phylogenetic analysis and tissue expression pattern. By sequence analysis, these five cynomolgus P450s had high sequence identities (94-99%) to the human orthologs in amino acids. By phylogenetic analysis, each cynomolgus P450 was more closely related to the human ortholog as compared with the dog or rat ortholog. By quantitative polymerase chain reaction, among the 10 tissue types, CYP7A1 and CYP17A1 mRNAs were preferentially expressed in liver and adrenal gland, respectively. Cynomolgus CYP27A1 and CYP51A1 mRNAs were most abundantly expressed in liver and testis, respectively. Cynomolgus CYP20A1 mRNA was expressed in all the tissues, including brain and liver. Tissue expression patterns of each cynomolgus P450 were generally similar to that of the human ortholog. These results suggest the molecular similarities of CYP7A1, CYP17A1, CYP20A1, CYP27A1 and CYP51A1 between cynomolgus macaques and humans.

Show MeSH
Related in: MedlinePlus