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Molecular cloning and gene expression of canine apoptosis inhibitor of macrophage.

Tomura S, Uchida M, Yonezawa T, Kobayashi M, Bonkobara M, Arai S, Miyazaki T, Tamahara S, Matsuki N - J. Vet. Med. Sci. (2014)

Bottom Line: Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages.CD36, the receptor of AIM, was also expressed in various tissues and these cell lines.These findings are useful to reveal the actual functions of canine AIM.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

ABSTRACT
Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

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Expression levels of AIM and CD36 mRNA in eight histiocytic sarcoma cell lines. Thesegene expression levels were normalized to GAPDH. The columns and error bars representthe means and their standard errors.
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fig_004: Expression levels of AIM and CD36 mRNA in eight histiocytic sarcoma cell lines. Thesegene expression levels were normalized to GAPDH. The columns and error bars representthe means and their standard errors.

Mentions: Canine histiocytic sarcoma is a tumor derived from macrophages, dendritic cells, monocytesand Langerhans cells [1, 15]. In this study, expression ratios of AIM and CD36 mRNA in several histiocyticsarcoma cell lines were observed. The cell lines were established by Dr. Azakami [3]. RNA samples were extracted from three different passagesof each cell line, and the quantities were measured using qPCR. As shown in Fig. 4Fig. 4.


Molecular cloning and gene expression of canine apoptosis inhibitor of macrophage.

Tomura S, Uchida M, Yonezawa T, Kobayashi M, Bonkobara M, Arai S, Miyazaki T, Tamahara S, Matsuki N - J. Vet. Med. Sci. (2014)

Expression levels of AIM and CD36 mRNA in eight histiocytic sarcoma cell lines. Thesegene expression levels were normalized to GAPDH. The columns and error bars representthe means and their standard errors.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300382&req=5

fig_004: Expression levels of AIM and CD36 mRNA in eight histiocytic sarcoma cell lines. Thesegene expression levels were normalized to GAPDH. The columns and error bars representthe means and their standard errors.
Mentions: Canine histiocytic sarcoma is a tumor derived from macrophages, dendritic cells, monocytesand Langerhans cells [1, 15]. In this study, expression ratios of AIM and CD36 mRNA in several histiocyticsarcoma cell lines were observed. The cell lines were established by Dr. Azakami [3]. RNA samples were extracted from three different passagesof each cell line, and the quantities were measured using qPCR. As shown in Fig. 4Fig. 4.

Bottom Line: Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages.CD36, the receptor of AIM, was also expressed in various tissues and these cell lines.These findings are useful to reveal the actual functions of canine AIM.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

ABSTRACT
Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

Show MeSH
Related in: MedlinePlus