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Molecular cloning and gene expression of canine apoptosis inhibitor of macrophage.

Tomura S, Uchida M, Yonezawa T, Kobayashi M, Bonkobara M, Arai S, Miyazaki T, Tamahara S, Matsuki N - J. Vet. Med. Sci. (2014)

Bottom Line: Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages.CD36, the receptor of AIM, was also expressed in various tissues and these cell lines.These findings are useful to reveal the actual functions of canine AIM.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

ABSTRACT
Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

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Related in: MedlinePlus

Alignment of the deduced amino acid sequence of the CDS of canine AIM with those ofhuman, mouse and rat homologs. The deduced amino acid sequence of canine AIM cDNA clonedin this study was shown to have 66.2%, 61.0% and 64.5% similarity with those of thehuman, mouse and rat counterparts, respectively. Asterisks indicate identical aminoacids. Colons and periods indicate strong and weak conservation of similar amino acidproperties. The predicted signal peptide of canine AIM is marked by an underline.Cysteine residues are shown on a black background. The three presumed SRCR domains areboxed with solid lines.
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fig_002: Alignment of the deduced amino acid sequence of the CDS of canine AIM with those ofhuman, mouse and rat homologs. The deduced amino acid sequence of canine AIM cDNA clonedin this study was shown to have 66.2%, 61.0% and 64.5% similarity with those of thehuman, mouse and rat counterparts, respectively. Asterisks indicate identical aminoacids. Colons and periods indicate strong and weak conservation of similar amino acidproperties. The predicted signal peptide of canine AIM is marked by an underline.Cysteine residues are shown on a black background. The three presumed SRCR domains areboxed with solid lines.

Mentions: Nucleotide and deduced amino acid sequences of canine AIM (accession number:AB_915633). The CDS of canine AIM was composed of 1,023 bp encoding 340 amino acidsresidues. The predicted signal peptide of canine AIM is marked by an underline. Thethree presumed SRCR domains are boxed with solid lines. Predicted N-linked glycosylationsites were shown as circles.


Molecular cloning and gene expression of canine apoptosis inhibitor of macrophage.

Tomura S, Uchida M, Yonezawa T, Kobayashi M, Bonkobara M, Arai S, Miyazaki T, Tamahara S, Matsuki N - J. Vet. Med. Sci. (2014)

Alignment of the deduced amino acid sequence of the CDS of canine AIM with those ofhuman, mouse and rat homologs. The deduced amino acid sequence of canine AIM cDNA clonedin this study was shown to have 66.2%, 61.0% and 64.5% similarity with those of thehuman, mouse and rat counterparts, respectively. Asterisks indicate identical aminoacids. Colons and periods indicate strong and weak conservation of similar amino acidproperties. The predicted signal peptide of canine AIM is marked by an underline.Cysteine residues are shown on a black background. The three presumed SRCR domains areboxed with solid lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300382&req=5

fig_002: Alignment of the deduced amino acid sequence of the CDS of canine AIM with those ofhuman, mouse and rat homologs. The deduced amino acid sequence of canine AIM cDNA clonedin this study was shown to have 66.2%, 61.0% and 64.5% similarity with those of thehuman, mouse and rat counterparts, respectively. Asterisks indicate identical aminoacids. Colons and periods indicate strong and weak conservation of similar amino acidproperties. The predicted signal peptide of canine AIM is marked by an underline.Cysteine residues are shown on a black background. The three presumed SRCR domains areboxed with solid lines.
Mentions: Nucleotide and deduced amino acid sequences of canine AIM (accession number:AB_915633). The CDS of canine AIM was composed of 1,023 bp encoding 340 amino acidsresidues. The predicted signal peptide of canine AIM is marked by an underline. Thethree presumed SRCR domains are boxed with solid lines. Predicted N-linked glycosylationsites were shown as circles.

Bottom Line: Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages.CD36, the receptor of AIM, was also expressed in various tissues and these cell lines.These findings are useful to reveal the actual functions of canine AIM.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

ABSTRACT
Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

Show MeSH
Related in: MedlinePlus