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Effects of STX, a novel estrogen membrane receptor agonist, on GnRH-induced luteinizing hormone secretion from cultured bovine anterior pituitary cells.

Rudolf FO, Kadokawa H - J. Vet. Med. Sci. (2014)

Bottom Line: STX is an agonist for a recently characterized membrane estrogen receptor whose structure has not been identified.Estradiol (0.001 to 0.1 nM) significantly suppressed GnRH-stimulated LH secretion, whereas STX did not affect GnRH-stimulated LH secretion at any of the tested concentrations.In conclusion, STX, unlike estradiol, possesses no suppressive effect on GnRH-induced LH release from bovine AP cells.

View Article: PubMed Central - PubMed

Affiliation: Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8511, Japan.

ABSTRACT
STX is an agonist for a recently characterized membrane estrogen receptor whose structure has not been identified. We evaluated whether STX suppresses gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone (LH) release from bovine anterior pituitary (AP) cells. We cultured AP cells (n=12) for 3 days in steroid-free conditions, followed by increasing concentrations (0.001, 0.01, 0.1, 1 and 10 nM) of 17β-estradiol or STX for 5 min before GnRH stimulation until the end of the experiment. Estradiol (0.001 to 0.1 nM) significantly suppressed GnRH-stimulated LH secretion, whereas STX did not affect GnRH-stimulated LH secretion at any of the tested concentrations. In conclusion, STX, unlike estradiol, possesses no suppressive effect on GnRH-induced LH release from bovine AP cells.

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Effects of various concentrations of estradiol (black bars) or STX (gray bars) in DMEMcontaining 1 nM GnRH on luteinizing hormone (LH) secretion from cultured bovine APcells. The LH concentrations in control cells (cultured medium only) were averaged withthe mean value set at 100%. The LH concentrations of the treated groups are expressed asa percentage of the control. Each value represents the mean concentration ± SEM (n=12).+P<0.05, ++P<0.01:significant differences compared with the control,†P<0.05, ††P<0.01:significant difference compared with GnRH, **P<0.01: significantdifferences compared with 10 nM estradiol.
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fig_001: Effects of various concentrations of estradiol (black bars) or STX (gray bars) in DMEMcontaining 1 nM GnRH on luteinizing hormone (LH) secretion from cultured bovine APcells. The LH concentrations in control cells (cultured medium only) were averaged withthe mean value set at 100%. The LH concentrations of the treated groups are expressed asa percentage of the control. Each value represents the mean concentration ± SEM (n=12).+P<0.05, ++P<0.01:significant differences compared with the control,†P<0.05, ††P<0.01:significant difference compared with GnRH, **P<0.01: significantdifferences compared with 10 nM estradiol.

Mentions: Figure 1Fig. 1.


Effects of STX, a novel estrogen membrane receptor agonist, on GnRH-induced luteinizing hormone secretion from cultured bovine anterior pituitary cells.

Rudolf FO, Kadokawa H - J. Vet. Med. Sci. (2014)

Effects of various concentrations of estradiol (black bars) or STX (gray bars) in DMEMcontaining 1 nM GnRH on luteinizing hormone (LH) secretion from cultured bovine APcells. The LH concentrations in control cells (cultured medium only) were averaged withthe mean value set at 100%. The LH concentrations of the treated groups are expressed asa percentage of the control. Each value represents the mean concentration ± SEM (n=12).+P<0.05, ++P<0.01:significant differences compared with the control,†P<0.05, ††P<0.01:significant difference compared with GnRH, **P<0.01: significantdifferences compared with 10 nM estradiol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300378&req=5

fig_001: Effects of various concentrations of estradiol (black bars) or STX (gray bars) in DMEMcontaining 1 nM GnRH on luteinizing hormone (LH) secretion from cultured bovine APcells. The LH concentrations in control cells (cultured medium only) were averaged withthe mean value set at 100%. The LH concentrations of the treated groups are expressed asa percentage of the control. Each value represents the mean concentration ± SEM (n=12).+P<0.05, ++P<0.01:significant differences compared with the control,†P<0.05, ††P<0.01:significant difference compared with GnRH, **P<0.01: significantdifferences compared with 10 nM estradiol.
Mentions: Figure 1Fig. 1.

Bottom Line: STX is an agonist for a recently characterized membrane estrogen receptor whose structure has not been identified.Estradiol (0.001 to 0.1 nM) significantly suppressed GnRH-stimulated LH secretion, whereas STX did not affect GnRH-stimulated LH secretion at any of the tested concentrations.In conclusion, STX, unlike estradiol, possesses no suppressive effect on GnRH-induced LH release from bovine AP cells.

View Article: PubMed Central - PubMed

Affiliation: Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8511, Japan.

ABSTRACT
STX is an agonist for a recently characterized membrane estrogen receptor whose structure has not been identified. We evaluated whether STX suppresses gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone (LH) release from bovine anterior pituitary (AP) cells. We cultured AP cells (n=12) for 3 days in steroid-free conditions, followed by increasing concentrations (0.001, 0.01, 0.1, 1 and 10 nM) of 17β-estradiol or STX for 5 min before GnRH stimulation until the end of the experiment. Estradiol (0.001 to 0.1 nM) significantly suppressed GnRH-stimulated LH secretion, whereas STX did not affect GnRH-stimulated LH secretion at any of the tested concentrations. In conclusion, STX, unlike estradiol, possesses no suppressive effect on GnRH-induced LH release from bovine AP cells.

Show MeSH
Related in: MedlinePlus