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Systemic mycobacteriosis in an aborted thoroughbred fetus in Japan.

Sano Y, Matsuda K, Osaki K, Miyasho T, Tsuda T, Taniyama H - J. Vet. Med. Sci. (2014)

Bottom Line: A male Thoroughbred fetus was aborted on day 251 of pregnancy.Gross and histological examinations detected systemic granulomatous lesions in many superficial and visceral lymph nodes and organs including the liver, tonsils, lungs, thymus, spleen, right thyroid gland and gastrointestinal tract, and suppurative placentitis, pyogranulomatous amnionitis and intralesional acid-fast bacilli were also detected.An examination of the DNA base sequence of the β subunit of RNA polymerase demonstrated that Mycobacterium avium strain 104 had infected several organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

ABSTRACT
A male Thoroughbred fetus was aborted on day 251 of pregnancy. Gross and histological examinations detected systemic granulomatous lesions in many superficial and visceral lymph nodes and organs including the liver, tonsils, lungs, thymus, spleen, right thyroid gland and gastrointestinal tract, and suppurative placentitis, pyogranulomatous amnionitis and intralesional acid-fast bacilli were also detected. An examination of the DNA base sequence of the β subunit of RNA polymerase demonstrated that Mycobacterium avium strain 104 had infected several organs. To the best of our knowledge, this is the first report of equine fetal mycobacterial infection in Japan.

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PCR analysis targeting the rpoβ gene. M: 100 bp DNA ladder, lanes 1–5:DNA samples obtained from the liver, lungs, cranial mesenteric lymph nodes, amnion andchorion, respectively. All PCR products are approximately 350 base pairs in length.
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fig_006: PCR analysis targeting the rpoβ gene. M: 100 bp DNA ladder, lanes 1–5:DNA samples obtained from the liver, lungs, cranial mesenteric lymph nodes, amnion andchorion, respectively. All PCR products are approximately 350 base pairs in length.

Mentions: Cryopreserved specimens of the thymus, lungs, liver, spleen, cranial mesenteric lymph nodes,allantochorion and allantoamnion that were obtained during necropsy were subjected topolymerase chain reaction (PCR) analysis to identify the acid-fast organisms. DNA wasextracted from the samples using a DNA isolation kit (MagNA Pure LC DNA Isolation Kit I,Roche, Basel, Switzerland) and was then analyzed by PCR targeting the gene encoding the βsubunit of RNA polymerase (rpoB) (primers: MF, 5′CGACCACTTCGGCAACCG3′; MR,5′TCGATCGGGCACATCCGG3′), as described previously [10].The resultant PCR product was approximately 350 base pairs in length (Fig. 6Fig. 6.


Systemic mycobacteriosis in an aborted thoroughbred fetus in Japan.

Sano Y, Matsuda K, Osaki K, Miyasho T, Tsuda T, Taniyama H - J. Vet. Med. Sci. (2014)

PCR analysis targeting the rpoβ gene. M: 100 bp DNA ladder, lanes 1–5:DNA samples obtained from the liver, lungs, cranial mesenteric lymph nodes, amnion andchorion, respectively. All PCR products are approximately 350 base pairs in length.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300377&req=5

fig_006: PCR analysis targeting the rpoβ gene. M: 100 bp DNA ladder, lanes 1–5:DNA samples obtained from the liver, lungs, cranial mesenteric lymph nodes, amnion andchorion, respectively. All PCR products are approximately 350 base pairs in length.
Mentions: Cryopreserved specimens of the thymus, lungs, liver, spleen, cranial mesenteric lymph nodes,allantochorion and allantoamnion that were obtained during necropsy were subjected topolymerase chain reaction (PCR) analysis to identify the acid-fast organisms. DNA wasextracted from the samples using a DNA isolation kit (MagNA Pure LC DNA Isolation Kit I,Roche, Basel, Switzerland) and was then analyzed by PCR targeting the gene encoding the βsubunit of RNA polymerase (rpoB) (primers: MF, 5′CGACCACTTCGGCAACCG3′; MR,5′TCGATCGGGCACATCCGG3′), as described previously [10].The resultant PCR product was approximately 350 base pairs in length (Fig. 6Fig. 6.

Bottom Line: A male Thoroughbred fetus was aborted on day 251 of pregnancy.Gross and histological examinations detected systemic granulomatous lesions in many superficial and visceral lymph nodes and organs including the liver, tonsils, lungs, thymus, spleen, right thyroid gland and gastrointestinal tract, and suppurative placentitis, pyogranulomatous amnionitis and intralesional acid-fast bacilli were also detected.An examination of the DNA base sequence of the β subunit of RNA polymerase demonstrated that Mycobacterium avium strain 104 had infected several organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

ABSTRACT
A male Thoroughbred fetus was aborted on day 251 of pregnancy. Gross and histological examinations detected systemic granulomatous lesions in many superficial and visceral lymph nodes and organs including the liver, tonsils, lungs, thymus, spleen, right thyroid gland and gastrointestinal tract, and suppurative placentitis, pyogranulomatous amnionitis and intralesional acid-fast bacilli were also detected. An examination of the DNA base sequence of the β subunit of RNA polymerase demonstrated that Mycobacterium avium strain 104 had infected several organs. To the best of our knowledge, this is the first report of equine fetal mycobacterial infection in Japan.

Show MeSH
Related in: MedlinePlus