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Prevalence and genotyping of Cryptosporidium spp. in farm animals in Egypt.

Mahfouz ME, Mira N, Amer S - J. Vet. Med. Sci. (2014)

Bottom Line: Genotypes distribution showed that C. ryanae was the dominant species (60.0%) followed by C. parvum (40.0%) in buffalo calves.Meanwhile, in cattle calves, C. parvum was the commonest species (74.23%) followed by C. ryanae (16.10%) and C. bovis (9.70%).Subtyping of C. parvum based on sequence analysis of the polymorphic 60 kDa glycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in both buffalo and cattle calves, addressing the potential role of calves in zoonotic cryptosporidiosis in Egypt.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr El Sheikh, 33516, Egypt.

ABSTRACT
In this study, we examined the prevalence and molecular characteristics of Cryptosporidium in buffalo, dairy cattle and sheep in different farms at Kafr El Sheikh Province, Egypt. Rectal fecal samples, including 466 samples from buffalo, 1697 from cattle and 120 from sheep, were collected from different ages and screened by modified Ziehl-Neelsen acid-fast microscopy for detection of Cryptosporidium oocysts. All studied farms were positives with an overall prevalence of 1.29% in buffalo (4.17% in claves versus 0.48% in adults), 7.07% in cattle (6.90% in calves versus 10.20% and 6.10% in heifers and adults, respectively) and 2.50% in sheep (4.40% in lambs versus 1.30% in adults). PCR-RFLP analyses of small-subunit rRNA genes from positive specimens revealed the occurrence of C. parvum and C. ryanae in buffalo; C. parvum, C. ryanae, C. bovis and C. andersoni in cattle and only C. xiaoi in sheep. Genotypes distribution showed that C. ryanae was the dominant species (60.0%) followed by C. parvum (40.0%) in buffalo calves. Meanwhile, in cattle calves, C. parvum was the commonest species (74.23%) followed by C. ryanae (16.10%) and C. bovis (9.70%). Subtyping of C. parvum based on sequence analysis of the polymorphic 60 kDa glycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in both buffalo and cattle calves, addressing the potential role of calves in zoonotic cryptosporidiosis in Egypt.

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Phylogenetic relationship of Cryptosporidium spp. based on sequencesof the partial SSU rRNA gene. Evolutionary relationships of 43 taxa were inferredusing the Neighbor-Joining method [39] with thecoccidian Eimeria tenella (AF026388) as an out-group. Numbers at theinternodes correspond to percent bootstrap values from 2,000 replicates. Egyptianisolates are bolded.
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fig_001: Phylogenetic relationship of Cryptosporidium spp. based on sequencesof the partial SSU rRNA gene. Evolutionary relationships of 43 taxa were inferredusing the Neighbor-Joining method [39] with thecoccidian Eimeria tenella (AF026388) as an out-group. Numbers at theinternodes correspond to percent bootstrap values from 2,000 replicates. Egyptianisolates are bolded.

Mentions: DNA sequencing of representative samples confirmed the identification of theCryptosporidium species. Blasting the obtained sequences with those indatabase and the deduced phylogenetic analysis (Fig.1Fig. 1.


Prevalence and genotyping of Cryptosporidium spp. in farm animals in Egypt.

Mahfouz ME, Mira N, Amer S - J. Vet. Med. Sci. (2014)

Phylogenetic relationship of Cryptosporidium spp. based on sequencesof the partial SSU rRNA gene. Evolutionary relationships of 43 taxa were inferredusing the Neighbor-Joining method [39] with thecoccidian Eimeria tenella (AF026388) as an out-group. Numbers at theinternodes correspond to percent bootstrap values from 2,000 replicates. Egyptianisolates are bolded.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300370&req=5

fig_001: Phylogenetic relationship of Cryptosporidium spp. based on sequencesof the partial SSU rRNA gene. Evolutionary relationships of 43 taxa were inferredusing the Neighbor-Joining method [39] with thecoccidian Eimeria tenella (AF026388) as an out-group. Numbers at theinternodes correspond to percent bootstrap values from 2,000 replicates. Egyptianisolates are bolded.
Mentions: DNA sequencing of representative samples confirmed the identification of theCryptosporidium species. Blasting the obtained sequences with those indatabase and the deduced phylogenetic analysis (Fig.1Fig. 1.

Bottom Line: Genotypes distribution showed that C. ryanae was the dominant species (60.0%) followed by C. parvum (40.0%) in buffalo calves.Meanwhile, in cattle calves, C. parvum was the commonest species (74.23%) followed by C. ryanae (16.10%) and C. bovis (9.70%).Subtyping of C. parvum based on sequence analysis of the polymorphic 60 kDa glycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in both buffalo and cattle calves, addressing the potential role of calves in zoonotic cryptosporidiosis in Egypt.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr El Sheikh, 33516, Egypt.

ABSTRACT
In this study, we examined the prevalence and molecular characteristics of Cryptosporidium in buffalo, dairy cattle and sheep in different farms at Kafr El Sheikh Province, Egypt. Rectal fecal samples, including 466 samples from buffalo, 1697 from cattle and 120 from sheep, were collected from different ages and screened by modified Ziehl-Neelsen acid-fast microscopy for detection of Cryptosporidium oocysts. All studied farms were positives with an overall prevalence of 1.29% in buffalo (4.17% in claves versus 0.48% in adults), 7.07% in cattle (6.90% in calves versus 10.20% and 6.10% in heifers and adults, respectively) and 2.50% in sheep (4.40% in lambs versus 1.30% in adults). PCR-RFLP analyses of small-subunit rRNA genes from positive specimens revealed the occurrence of C. parvum and C. ryanae in buffalo; C. parvum, C. ryanae, C. bovis and C. andersoni in cattle and only C. xiaoi in sheep. Genotypes distribution showed that C. ryanae was the dominant species (60.0%) followed by C. parvum (40.0%) in buffalo calves. Meanwhile, in cattle calves, C. parvum was the commonest species (74.23%) followed by C. ryanae (16.10%) and C. bovis (9.70%). Subtyping of C. parvum based on sequence analysis of the polymorphic 60 kDa glycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in both buffalo and cattle calves, addressing the potential role of calves in zoonotic cryptosporidiosis in Egypt.

Show MeSH
Related in: MedlinePlus