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Comparison of surgical methods of transient middle cerebral artery occlusion between rats and mice.

Lee S, Hong Y, Park S, Lee SR, Chang KT, Hong Y - J. Vet. Med. Sci. (2014)

Bottom Line: In rodent MCAo models, has to be considered body temperature during the operative period, as well as the need for the use of a standardized tip in terms of the outer diameter of probes.Our methods could induce stable moderate-severity ischemic brain injury models and histological alteration at 24 hr after MCAo surgery.Finally, we described and compared major parameters between rats and mice, including probe size, thread insert length, operation and occlusion periods, and differences in the procedures.

View Article: PubMed Central - PubMed

Affiliation: Biohealth Products Research Center (BPRC), Inje University, Gimhae, Korea.

ABSTRACT
Rodent models of focal cerebral ischemia that do not require craniotomy have been developed by intraluminal suture middle cerebral artery occlusion (MCAo). Mouse MCAo models have been widely used and extended to genetic studies of cell death or recovery mechanisms. Therefore, we compared surgery-related parameters and techniques between such rats and mice. In rodent MCAo models, has to be considered body temperature during the operative period, as well as the need for the use of a standardized tip in terms of the outer diameter of probes. Induction of focal cerebral ischemia was measured by neurological dysfunction parameters. Our methods could induce stable moderate-severity ischemic brain injury models and histological alteration at 24 hr after MCAo surgery. Moreover approximately 80% (rats) and 85% (mice) survival ratios were shown indicating with model engineering success. Finally, we described and compared major parameters between rats and mice, including probe size, thread insert length, operation and occlusion periods, and differences in the procedures.

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Results of body weight alteration and neurological dysfunction symptoms. (A)Alteration of body weight of rat MCAo models followed by an occlusion period. (B)Alteration of body weight of mouse MCAo models followed by an occlusion period. (C)Neurological severity score of rat MCAo models at 24 hr after transient MCAo surgeryby occlusion period. (D) Neurological disability status of mouse MCAo models at 24 hrafter transient MCAo surgery by occlusion period. Occlusion period did notsignificantly affect neurological score. The 90 min occlusion period was clearlyassociated with a severe score, which affected survival ratio and model identity.mNSS: modified neurological severity score; NDSS: neurological disability statusscore. *P<0.05, **P<0.01 compared with that atonset, Student’s t-test.
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fig_002: Results of body weight alteration and neurological dysfunction symptoms. (A)Alteration of body weight of rat MCAo models followed by an occlusion period. (B)Alteration of body weight of mouse MCAo models followed by an occlusion period. (C)Neurological severity score of rat MCAo models at 24 hr after transient MCAo surgeryby occlusion period. (D) Neurological disability status of mouse MCAo models at 24 hrafter transient MCAo surgery by occlusion period. Occlusion period did notsignificantly affect neurological score. The 90 min occlusion period was clearlyassociated with a severe score, which affected survival ratio and model identity.mNSS: modified neurological severity score; NDSS: neurological disability statusscore. *P<0.05, **P<0.01 compared with that atonset, Student’s t-test.

Mentions: Alteration of body weight in rodent MCAo animal models: MCAo surgery wasapplied to 8-week-old rats and mice, as well as equalization of the body weight from 240–260g (rats; 60 min: 253.08 ± 5.62 g and 90 min: 256.89 ± 2.26 g) and 20–25 g (mice; 60 min:23.30 ± 0.79 g and 90 min: 23.50 ± 1.19 g). The subjects showed significant reductions ofbody weight compared with those before onset (rats; 60 min: 23.70 ± 7.43 g and 90 min: 36.29± 9.31 g) (mice; 60 min: 2.03 ± 0.54 g and 90 min: 3.17 ± 1.11 g)(P<0.01) (Fig. 2A, BFig. 2.


Comparison of surgical methods of transient middle cerebral artery occlusion between rats and mice.

Lee S, Hong Y, Park S, Lee SR, Chang KT, Hong Y - J. Vet. Med. Sci. (2014)

Results of body weight alteration and neurological dysfunction symptoms. (A)Alteration of body weight of rat MCAo models followed by an occlusion period. (B)Alteration of body weight of mouse MCAo models followed by an occlusion period. (C)Neurological severity score of rat MCAo models at 24 hr after transient MCAo surgeryby occlusion period. (D) Neurological disability status of mouse MCAo models at 24 hrafter transient MCAo surgery by occlusion period. Occlusion period did notsignificantly affect neurological score. The 90 min occlusion period was clearlyassociated with a severe score, which affected survival ratio and model identity.mNSS: modified neurological severity score; NDSS: neurological disability statusscore. *P<0.05, **P<0.01 compared with that atonset, Student’s t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300368&req=5

fig_002: Results of body weight alteration and neurological dysfunction symptoms. (A)Alteration of body weight of rat MCAo models followed by an occlusion period. (B)Alteration of body weight of mouse MCAo models followed by an occlusion period. (C)Neurological severity score of rat MCAo models at 24 hr after transient MCAo surgeryby occlusion period. (D) Neurological disability status of mouse MCAo models at 24 hrafter transient MCAo surgery by occlusion period. Occlusion period did notsignificantly affect neurological score. The 90 min occlusion period was clearlyassociated with a severe score, which affected survival ratio and model identity.mNSS: modified neurological severity score; NDSS: neurological disability statusscore. *P<0.05, **P<0.01 compared with that atonset, Student’s t-test.
Mentions: Alteration of body weight in rodent MCAo animal models: MCAo surgery wasapplied to 8-week-old rats and mice, as well as equalization of the body weight from 240–260g (rats; 60 min: 253.08 ± 5.62 g and 90 min: 256.89 ± 2.26 g) and 20–25 g (mice; 60 min:23.30 ± 0.79 g and 90 min: 23.50 ± 1.19 g). The subjects showed significant reductions ofbody weight compared with those before onset (rats; 60 min: 23.70 ± 7.43 g and 90 min: 36.29± 9.31 g) (mice; 60 min: 2.03 ± 0.54 g and 90 min: 3.17 ± 1.11 g)(P<0.01) (Fig. 2A, BFig. 2.

Bottom Line: In rodent MCAo models, has to be considered body temperature during the operative period, as well as the need for the use of a standardized tip in terms of the outer diameter of probes.Our methods could induce stable moderate-severity ischemic brain injury models and histological alteration at 24 hr after MCAo surgery.Finally, we described and compared major parameters between rats and mice, including probe size, thread insert length, operation and occlusion periods, and differences in the procedures.

View Article: PubMed Central - PubMed

Affiliation: Biohealth Products Research Center (BPRC), Inje University, Gimhae, Korea.

ABSTRACT
Rodent models of focal cerebral ischemia that do not require craniotomy have been developed by intraluminal suture middle cerebral artery occlusion (MCAo). Mouse MCAo models have been widely used and extended to genetic studies of cell death or recovery mechanisms. Therefore, we compared surgery-related parameters and techniques between such rats and mice. In rodent MCAo models, has to be considered body temperature during the operative period, as well as the need for the use of a standardized tip in terms of the outer diameter of probes. Induction of focal cerebral ischemia was measured by neurological dysfunction parameters. Our methods could induce stable moderate-severity ischemic brain injury models and histological alteration at 24 hr after MCAo surgery. Moreover approximately 80% (rats) and 85% (mice) survival ratios were shown indicating with model engineering success. Finally, we described and compared major parameters between rats and mice, including probe size, thread insert length, operation and occlusion periods, and differences in the procedures.

Show MeSH
Related in: MedlinePlus