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Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis.

Guha Thakurta S, Budhiraja G, Subramanian A - J Tissue Eng (2015)

Bottom Line: Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion.The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2).No COL10A1 protein expression was noted.

View Article: PubMed Central - PubMed

Affiliation: Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

ABSTRACT
Ultrasound at 5.0 MHz was noted to be chondro-inductive, with improved SOX-9 gene and COL2A1 protein expression in constructs that allowed for cell-to-cell contact. To achieve tissue-engineered cartilage using macroporous scaffolds, it is hypothesized that a combination of ultrasound at 5.0 MHz and transforming growth factor-β3 induces human mesenchymal stem cell differentiation to chondrocytes. Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion. Our results suggest that in group 1 (no transforming growth factor-β3, no ultrasound), as anticipated, human mesenchymal stem cells were not efficiently differentiated into chondrocytes, judging by the lack of decrease in the level of miR-145 expression. Human mesenchymal stem cells differentiated into chondrocytes in group 2 (transforming growth factor-β3, no ultrasound) and group 3 (transforming growth factor-β3, ultrasound) with group 3 having a 2-fold lower miR-145 when compared to group 2 at day 7, indicating a higher conversion to chondrocytes. Transforming growth factor-β3-induced chondrogenesis with and without ultrasound stimulation for 14 days in the ultrasound-assisted bioreactor was compared and followed by additional culture in the absence of growth factors. The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2). No COL10A1 protein expression was noted. Enhanced cell proliferation and glycosaminoglycan deposition was noted with the combination of growth factor and ultrasound stimulation. These results suggest that ultrasound at 5.0 MHz could be used to induce chondrogenic differentiation of mesenchymal stem cells for cartilage tissue engineering.

No MeSH data available.


Related in: MedlinePlus

TGFβ3 dose in CDM over 1–21 days. hMSCs were seeded on Biomerix™ scaffolds (2 × 105 cells/scaffold, 5 mm × 2.5 mm) and cultured in the US-assisted bioreactor. The following independent study groups (Table 1) were adopted: Group 1—No TGFβ3, No US (Control); Group 2—TGFβ3, No US; and Group 3—TGFβ3, US. TGFβ3 concentrations used are indicated above. After 14 days of culture in M2 or CDM, cell-constructs were cultured for an additional week in M3.
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fig1-2041731414566529: TGFβ3 dose in CDM over 1–21 days. hMSCs were seeded on Biomerix™ scaffolds (2 × 105 cells/scaffold, 5 mm × 2.5 mm) and cultured in the US-assisted bioreactor. The following independent study groups (Table 1) were adopted: Group 1—No TGFβ3, No US (Control); Group 2—TGFβ3, No US; and Group 3—TGFβ3, US. TGFβ3 concentrations used are indicated above. After 14 days of culture in M2 or CDM, cell-constructs were cultured for an additional week in M3.

Mentions: Medium 2 (denoted as M2) containing high-glucose DMEM, 10% FBS, 100 nM dexamethasone, 50 µg/mL of L-ascorbic Acid, and 1X antibiotic–antimyotic solution was further supplemented with TGFβ3 according to Figure 1 for chondrogenic differentiation and denoted as chondrogenic differentiation media (CDM; M2 + TGFβ3). After 14 days of culture, M2 or CDM was removed and cell-constructs were cultured for an additional 1 week in Medium 3 (M3) comprising M2 without dexamethasone (Table 1). All supplements and media for cell culture were purchased from Life Technologies (Grand Island, NY, USA).


Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis.

Guha Thakurta S, Budhiraja G, Subramanian A - J Tissue Eng (2015)

TGFβ3 dose in CDM over 1–21 days. hMSCs were seeded on Biomerix™ scaffolds (2 × 105 cells/scaffold, 5 mm × 2.5 mm) and cultured in the US-assisted bioreactor. The following independent study groups (Table 1) were adopted: Group 1—No TGFβ3, No US (Control); Group 2—TGFβ3, No US; and Group 3—TGFβ3, US. TGFβ3 concentrations used are indicated above. After 14 days of culture in M2 or CDM, cell-constructs were cultured for an additional week in M3.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4300305&req=5

fig1-2041731414566529: TGFβ3 dose in CDM over 1–21 days. hMSCs were seeded on Biomerix™ scaffolds (2 × 105 cells/scaffold, 5 mm × 2.5 mm) and cultured in the US-assisted bioreactor. The following independent study groups (Table 1) were adopted: Group 1—No TGFβ3, No US (Control); Group 2—TGFβ3, No US; and Group 3—TGFβ3, US. TGFβ3 concentrations used are indicated above. After 14 days of culture in M2 or CDM, cell-constructs were cultured for an additional week in M3.
Mentions: Medium 2 (denoted as M2) containing high-glucose DMEM, 10% FBS, 100 nM dexamethasone, 50 µg/mL of L-ascorbic Acid, and 1X antibiotic–antimyotic solution was further supplemented with TGFβ3 according to Figure 1 for chondrogenic differentiation and denoted as chondrogenic differentiation media (CDM; M2 + TGFβ3). After 14 days of culture, M2 or CDM was removed and cell-constructs were cultured for an additional 1 week in Medium 3 (M3) comprising M2 without dexamethasone (Table 1). All supplements and media for cell culture were purchased from Life Technologies (Grand Island, NY, USA).

Bottom Line: Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion.The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2).No COL10A1 protein expression was noted.

View Article: PubMed Central - PubMed

Affiliation: Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

ABSTRACT
Ultrasound at 5.0 MHz was noted to be chondro-inductive, with improved SOX-9 gene and COL2A1 protein expression in constructs that allowed for cell-to-cell contact. To achieve tissue-engineered cartilage using macroporous scaffolds, it is hypothesized that a combination of ultrasound at 5.0 MHz and transforming growth factor-β3 induces human mesenchymal stem cell differentiation to chondrocytes. Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion. Our results suggest that in group 1 (no transforming growth factor-β3, no ultrasound), as anticipated, human mesenchymal stem cells were not efficiently differentiated into chondrocytes, judging by the lack of decrease in the level of miR-145 expression. Human mesenchymal stem cells differentiated into chondrocytes in group 2 (transforming growth factor-β3, no ultrasound) and group 3 (transforming growth factor-β3, ultrasound) with group 3 having a 2-fold lower miR-145 when compared to group 2 at day 7, indicating a higher conversion to chondrocytes. Transforming growth factor-β3-induced chondrogenesis with and without ultrasound stimulation for 14 days in the ultrasound-assisted bioreactor was compared and followed by additional culture in the absence of growth factors. The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2). No COL10A1 protein expression was noted. Enhanced cell proliferation and glycosaminoglycan deposition was noted with the combination of growth factor and ultrasound stimulation. These results suggest that ultrasound at 5.0 MHz could be used to induce chondrogenic differentiation of mesenchymal stem cells for cartilage tissue engineering.

No MeSH data available.


Related in: MedlinePlus