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Dual ameliorative effects of Ningdong granule on dopamine in rat models of Tourette's syndrome.

Zhang F, Li A - Sci Rep (2015)

Bottom Line: Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions.By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment.Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Provincial Hospital affiliated to Shandong University, No.324, Jingwuweiqi Road, Jinan 250021, Shandong, PR, China.

ABSTRACT
Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions. Here, we use apomorphine (Apo) and 3,3'-iminodipropionitrile (IDPN) to develop two rat models of Tourette's syndrome (TS), a common neuropsychiatric disorder characterized by stereotyped repetitive involuntary tics. The models enabled the assessment of unique ameliorative effects of Ningdong granule (NDG), a traditional Chinese medicine (TCM) preparation dedicated to the treatment of TS, on the striatal DA content of rats. By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment. Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

No MeSH data available.


Related in: MedlinePlus

(a and b) Effects of NDG on striatal DRD2 protein content in experimental rats. Data are expressed as the mean ± SEM. (n = 10 rats/group). (a): Effect of NDG on striatal DRD2 protein content in Apo-induced TS rats. #p < 0.01 represents when compared to the control group; *p < 0.01 represents when compared to the Apo + NS group; ##p < 0.01 represents when compared to the Apo + NS group. (b): Effects of NDG on striatal DRD2 protein content in IDPN-induced TS rats. #p < 0.01, represents when compared to the control group; *p < 0.01, represents when compared to IDPN + NS group; ##p < 0.01, represents when compared to the IDPN + NS group.
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f3: (a and b) Effects of NDG on striatal DRD2 protein content in experimental rats. Data are expressed as the mean ± SEM. (n = 10 rats/group). (a): Effect of NDG on striatal DRD2 protein content in Apo-induced TS rats. #p < 0.01 represents when compared to the control group; *p < 0.01 represents when compared to the Apo + NS group; ##p < 0.01 represents when compared to the Apo + NS group. (b): Effects of NDG on striatal DRD2 protein content in IDPN-induced TS rats. #p < 0.01, represents when compared to the control group; *p < 0.01, represents when compared to IDPN + NS group; ##p < 0.01, represents when compared to the IDPN + NS group.

Mentions: The effects of NDG on the striatal DRD2 protein content of the rats are shown in Figure 3. The ANOVA revealed a significant effect for groups (Apo: F3,36 = 24.69, p < 0.01; IDPN: F3,36 = 29.73, p < 0.01). Apo and IDPN exposures were associated with significantly increased DRD2 protein content in the striatum compared with the same control group (Apo: p < 0.01; IDPN: p < 0.01). After being treated, in the Apo groups, both NDG and Hal treatment prevented the Apo-induced elevation of DRD2 protein content in striatum (Apo + NDG group vs Apo + NS group p < 0.01; Apo + Hal group vs Apo + NS group p < 0.01) (Figure 3a). Meanwhile, results similar to Apo groups were also observed in the IDPN groups after being treated with NDG and Hal respectively (IDPN + NDG group vs IDPN + NS group p < 0.01; IDPN + Hal group vs IDPN + NS group p < 0.01) (Figure 3b).


Dual ameliorative effects of Ningdong granule on dopamine in rat models of Tourette's syndrome.

Zhang F, Li A - Sci Rep (2015)

(a and b) Effects of NDG on striatal DRD2 protein content in experimental rats. Data are expressed as the mean ± SEM. (n = 10 rats/group). (a): Effect of NDG on striatal DRD2 protein content in Apo-induced TS rats. #p < 0.01 represents when compared to the control group; *p < 0.01 represents when compared to the Apo + NS group; ##p < 0.01 represents when compared to the Apo + NS group. (b): Effects of NDG on striatal DRD2 protein content in IDPN-induced TS rats. #p < 0.01, represents when compared to the control group; *p < 0.01, represents when compared to IDPN + NS group; ##p < 0.01, represents when compared to the IDPN + NS group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4296291&req=5

f3: (a and b) Effects of NDG on striatal DRD2 protein content in experimental rats. Data are expressed as the mean ± SEM. (n = 10 rats/group). (a): Effect of NDG on striatal DRD2 protein content in Apo-induced TS rats. #p < 0.01 represents when compared to the control group; *p < 0.01 represents when compared to the Apo + NS group; ##p < 0.01 represents when compared to the Apo + NS group. (b): Effects of NDG on striatal DRD2 protein content in IDPN-induced TS rats. #p < 0.01, represents when compared to the control group; *p < 0.01, represents when compared to IDPN + NS group; ##p < 0.01, represents when compared to the IDPN + NS group.
Mentions: The effects of NDG on the striatal DRD2 protein content of the rats are shown in Figure 3. The ANOVA revealed a significant effect for groups (Apo: F3,36 = 24.69, p < 0.01; IDPN: F3,36 = 29.73, p < 0.01). Apo and IDPN exposures were associated with significantly increased DRD2 protein content in the striatum compared with the same control group (Apo: p < 0.01; IDPN: p < 0.01). After being treated, in the Apo groups, both NDG and Hal treatment prevented the Apo-induced elevation of DRD2 protein content in striatum (Apo + NDG group vs Apo + NS group p < 0.01; Apo + Hal group vs Apo + NS group p < 0.01) (Figure 3a). Meanwhile, results similar to Apo groups were also observed in the IDPN groups after being treated with NDG and Hal respectively (IDPN + NDG group vs IDPN + NS group p < 0.01; IDPN + Hal group vs IDPN + NS group p < 0.01) (Figure 3b).

Bottom Line: Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions.By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment.Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Provincial Hospital affiliated to Shandong University, No.324, Jingwuweiqi Road, Jinan 250021, Shandong, PR, China.

ABSTRACT
Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions. Here, we use apomorphine (Apo) and 3,3'-iminodipropionitrile (IDPN) to develop two rat models of Tourette's syndrome (TS), a common neuropsychiatric disorder characterized by stereotyped repetitive involuntary tics. The models enabled the assessment of unique ameliorative effects of Ningdong granule (NDG), a traditional Chinese medicine (TCM) preparation dedicated to the treatment of TS, on the striatal DA content of rats. By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment. Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

No MeSH data available.


Related in: MedlinePlus