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Dual ameliorative effects of Ningdong granule on dopamine in rat models of Tourette's syndrome.

Zhang F, Li A - Sci Rep (2015)

Bottom Line: Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions.By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment.Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Provincial Hospital affiliated to Shandong University, No.324, Jingwuweiqi Road, Jinan 250021, Shandong, PR, China.

ABSTRACT
Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions. Here, we use apomorphine (Apo) and 3,3'-iminodipropionitrile (IDPN) to develop two rat models of Tourette's syndrome (TS), a common neuropsychiatric disorder characterized by stereotyped repetitive involuntary tics. The models enabled the assessment of unique ameliorative effects of Ningdong granule (NDG), a traditional Chinese medicine (TCM) preparation dedicated to the treatment of TS, on the striatal DA content of rats. By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment. Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

No MeSH data available.


Related in: MedlinePlus

(a and b) Evaluations of stereotyped behaviors of experimental rats during an 8-week period. Data are expressed as the mean ± SEM. (n = 10 rats/group). The initial scores before treatments showed no differences among groups (p > 0.05). However, after 3 weeks of treatments, both NDG and Hal made a significant decrease in ethological recording scores compared with the TS model rats treated with NS (Apo + NDG group vs Apo + NS group, p < 0.01; Apo + Hal group vs Apo + NS group, p < 0.01; IDPN + NDG group vs IDPN + NS group, p < 0.01; IDPN + Hal group vs IDPN + NS group, p < 0.05), while there was no remarkable differences in score recording between these two treatments in either Apo or IDPN induced group. (Apo + NDG group vs Apo + Hal group, p > 0.05; IDPN + NDG group vs IDPN + Hal group, p > 0.05). a: #p < 0.05, significant difference between Apo + NDG group and Apo + NS group; *p < 0.05, significant difference between Apo + Hal group and Apo + NS group. b: #p < 0.05, significant difference between IDPN + NDG group and IDPN + NS group; *p < 0.05, significant difference between IDPN + Hal group and IDPN + NS group.
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f1: (a and b) Evaluations of stereotyped behaviors of experimental rats during an 8-week period. Data are expressed as the mean ± SEM. (n = 10 rats/group). The initial scores before treatments showed no differences among groups (p > 0.05). However, after 3 weeks of treatments, both NDG and Hal made a significant decrease in ethological recording scores compared with the TS model rats treated with NS (Apo + NDG group vs Apo + NS group, p < 0.01; Apo + Hal group vs Apo + NS group, p < 0.01; IDPN + NDG group vs IDPN + NS group, p < 0.01; IDPN + Hal group vs IDPN + NS group, p < 0.05), while there was no remarkable differences in score recording between these two treatments in either Apo or IDPN induced group. (Apo + NDG group vs Apo + Hal group, p > 0.05; IDPN + NDG group vs IDPN + Hal group, p > 0.05). a: #p < 0.05, significant difference between Apo + NDG group and Apo + NS group; *p < 0.05, significant difference between Apo + Hal group and Apo + NS group. b: #p < 0.05, significant difference between IDPN + NDG group and IDPN + NS group; *p < 0.05, significant difference between IDPN + Hal group and IDPN + NS group.

Mentions: The effects of NDG on the stereotyped behaviors of the rats are shown in Figure 1. Using repeated measurements ANOVA, we found that both Apo and IDPN induced models had significant group effects (Apo: F2,27 = 22.73, p < 0.01; IDPN: F2,27 = 42.25, p < 0.01), indicating vary degrees of differences among Apo and IDPN groups. Compared to the same control rats shared by Apo and IDPN groups throughout the study, both of the pharmacological manipulations, Apo and IDPN, were associated with marked behavioral stereotypies in rats (Apo: p < 0.01; IDPN: p < 0.01). After being treated, in the rats exposed to Apo, long-term administration of NDG as well as Hal effectively rescued the Apo-induced stereotyped motor deficits (NDG: p < 0.01; Hal: p < 0.01) (Figure 1a). Meanwhile, in the rats subjected to IDPN, a steady reduction in stereotypies was also observed in stereotypies compared to the IDPN + NS group, after being treated by NDG and Hal respectively (NDG: p < 0.01; Hal: p < 0.01) (Figure 1b).


Dual ameliorative effects of Ningdong granule on dopamine in rat models of Tourette's syndrome.

Zhang F, Li A - Sci Rep (2015)

(a and b) Evaluations of stereotyped behaviors of experimental rats during an 8-week period. Data are expressed as the mean ± SEM. (n = 10 rats/group). The initial scores before treatments showed no differences among groups (p > 0.05). However, after 3 weeks of treatments, both NDG and Hal made a significant decrease in ethological recording scores compared with the TS model rats treated with NS (Apo + NDG group vs Apo + NS group, p < 0.01; Apo + Hal group vs Apo + NS group, p < 0.01; IDPN + NDG group vs IDPN + NS group, p < 0.01; IDPN + Hal group vs IDPN + NS group, p < 0.05), while there was no remarkable differences in score recording between these two treatments in either Apo or IDPN induced group. (Apo + NDG group vs Apo + Hal group, p > 0.05; IDPN + NDG group vs IDPN + Hal group, p > 0.05). a: #p < 0.05, significant difference between Apo + NDG group and Apo + NS group; *p < 0.05, significant difference between Apo + Hal group and Apo + NS group. b: #p < 0.05, significant difference between IDPN + NDG group and IDPN + NS group; *p < 0.05, significant difference between IDPN + Hal group and IDPN + NS group.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4296291&req=5

f1: (a and b) Evaluations of stereotyped behaviors of experimental rats during an 8-week period. Data are expressed as the mean ± SEM. (n = 10 rats/group). The initial scores before treatments showed no differences among groups (p > 0.05). However, after 3 weeks of treatments, both NDG and Hal made a significant decrease in ethological recording scores compared with the TS model rats treated with NS (Apo + NDG group vs Apo + NS group, p < 0.01; Apo + Hal group vs Apo + NS group, p < 0.01; IDPN + NDG group vs IDPN + NS group, p < 0.01; IDPN + Hal group vs IDPN + NS group, p < 0.05), while there was no remarkable differences in score recording between these two treatments in either Apo or IDPN induced group. (Apo + NDG group vs Apo + Hal group, p > 0.05; IDPN + NDG group vs IDPN + Hal group, p > 0.05). a: #p < 0.05, significant difference between Apo + NDG group and Apo + NS group; *p < 0.05, significant difference between Apo + Hal group and Apo + NS group. b: #p < 0.05, significant difference between IDPN + NDG group and IDPN + NS group; *p < 0.05, significant difference between IDPN + Hal group and IDPN + NS group.
Mentions: The effects of NDG on the stereotyped behaviors of the rats are shown in Figure 1. Using repeated measurements ANOVA, we found that both Apo and IDPN induced models had significant group effects (Apo: F2,27 = 22.73, p < 0.01; IDPN: F2,27 = 42.25, p < 0.01), indicating vary degrees of differences among Apo and IDPN groups. Compared to the same control rats shared by Apo and IDPN groups throughout the study, both of the pharmacological manipulations, Apo and IDPN, were associated with marked behavioral stereotypies in rats (Apo: p < 0.01; IDPN: p < 0.01). After being treated, in the rats exposed to Apo, long-term administration of NDG as well as Hal effectively rescued the Apo-induced stereotyped motor deficits (NDG: p < 0.01; Hal: p < 0.01) (Figure 1a). Meanwhile, in the rats subjected to IDPN, a steady reduction in stereotypies was also observed in stereotypies compared to the IDPN + NS group, after being treated by NDG and Hal respectively (NDG: p < 0.01; Hal: p < 0.01) (Figure 1b).

Bottom Line: Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions.By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment.Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Provincial Hospital affiliated to Shandong University, No.324, Jingwuweiqi Road, Jinan 250021, Shandong, PR, China.

ABSTRACT
Dopamine (DA) is a key neuromodulator in the brain that supports motor and cognitive functions. Here, we use apomorphine (Apo) and 3,3'-iminodipropionitrile (IDPN) to develop two rat models of Tourette's syndrome (TS), a common neuropsychiatric disorder characterized by stereotyped repetitive involuntary tics. The models enabled the assessment of unique ameliorative effects of Ningdong granule (NDG), a traditional Chinese medicine (TCM) preparation dedicated to the treatment of TS, on the striatal DA content of rats. By using high-performance liquid chromatography (HPLC), we found that long-term administration of NDG could, at least partially, restore the striatal dopamine alterations, either by increasing them after IDPN treatment or by decreasing them after Apo treatment. Taken together, our data indicated that NDG could ameliorate the abnormal striatal DA content dually, and the unique therapeutic property may be meaningful for the treatment of TS.

No MeSH data available.


Related in: MedlinePlus