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Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival.

Kobyakov DS, Avdalyan AM, Lazarev AF, Lushnikova EL, Nepomnyashchikh LM - Cancer Biol Med (2014)

Bottom Line: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area.Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.

View Article: PubMed Central - PubMed

Affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.

ABSTRACT

Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).

Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods.

Results: The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.

Conclusion: The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.

No MeSH data available.


Related in: MedlinePlus

AgNOR in MIB-1-positive and MIB-1-negative cells of NSCLC in squamous cell cancer with absence (А, B) and presence (C, D) of metastases to lymph nodes and in adenocarcinoma with well (E, F) and poor (G, H) differentiation. Double staining for Ki-67 (clone MIB-1) by immunohistochemistry and for AgNOR with silver nitrate, ×1,000. AgNOR, argyrophilic nucleolar organizer region; NSCLC, non-small-cell lung cancer.
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f1: AgNOR in MIB-1-positive and MIB-1-negative cells of NSCLC in squamous cell cancer with absence (А, B) and presence (C, D) of metastases to lymph nodes and in adenocarcinoma with well (E, F) and poor (G, H) differentiation. Double staining for Ki-67 (clone MIB-1) by immunohistochemistry and for AgNOR with silver nitrate, ×1,000. AgNOR, argyrophilic nucleolar organizer region; NSCLC, non-small-cell lung cancer.

Mentions: Immunohistochemical staining results of slides with primary antibodies for MIB-1 and subsequent staining results with silver nitrate were detected in the form of black round granules (AgNOR) located against a red nucleus (MIB-1-positive cells) or against a brown nucleolus or pale yellow nucleus (MIB-1-negative cells; Figure 1). In the nuclei, isolated black round granules (dots) appeared in the nucleoplasm (usually in MIB-1-negative cells) and/or multiple black round granules (dots) as a cluster (usually in MIB-1-positive cells). The morphometric results of the AgNOR area in MIB-1-positive cells in different clinicopathological parameters of NSCLC are shown in Table 1.


Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival.

Kobyakov DS, Avdalyan AM, Lazarev AF, Lushnikova EL, Nepomnyashchikh LM - Cancer Biol Med (2014)

AgNOR in MIB-1-positive and MIB-1-negative cells of NSCLC in squamous cell cancer with absence (А, B) and presence (C, D) of metastases to lymph nodes and in adenocarcinoma with well (E, F) and poor (G, H) differentiation. Double staining for Ki-67 (clone MIB-1) by immunohistochemistry and for AgNOR with silver nitrate, ×1,000. AgNOR, argyrophilic nucleolar organizer region; NSCLC, non-small-cell lung cancer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4296087&req=5

f1: AgNOR in MIB-1-positive and MIB-1-negative cells of NSCLC in squamous cell cancer with absence (А, B) and presence (C, D) of metastases to lymph nodes and in adenocarcinoma with well (E, F) and poor (G, H) differentiation. Double staining for Ki-67 (clone MIB-1) by immunohistochemistry and for AgNOR with silver nitrate, ×1,000. AgNOR, argyrophilic nucleolar organizer region; NSCLC, non-small-cell lung cancer.
Mentions: Immunohistochemical staining results of slides with primary antibodies for MIB-1 and subsequent staining results with silver nitrate were detected in the form of black round granules (AgNOR) located against a red nucleus (MIB-1-positive cells) or against a brown nucleolus or pale yellow nucleus (MIB-1-negative cells; Figure 1). In the nuclei, isolated black round granules (dots) appeared in the nucleoplasm (usually in MIB-1-negative cells) and/or multiple black round granules (dots) as a cluster (usually in MIB-1-positive cells). The morphometric results of the AgNOR area in MIB-1-positive cells in different clinicopathological parameters of NSCLC are shown in Table 1.

Bottom Line: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area.Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.

View Article: PubMed Central - PubMed

Affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.

ABSTRACT

Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).

Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods.

Results: The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.

Conclusion: The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.

No MeSH data available.


Related in: MedlinePlus