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On the benefit of galls of Quercus brantii Lindl. in murine colitis: the role of free gallic acid.

Khanavi M, Sabbagh-Bani-Azad M, Abdolghaffari AH, Vazirian M, Isazadeh I, Rezvanfar MA, Baeeri M, Mohammadirad A, Rahimi R, Shams-Ardekani MR, Abdollahi M - Arch Med Sci (2014)

Bottom Line: TNBS-induced colitis exhibited a significant increase in colon MPO activity and concentrations of cellular LPO, TNF-α and IL-1β, while TAP was significantly reduced.Parameters including macroscopic and microscopic scores, TNF-α, IL-1β, LPO, TAP and MPO improved by both gall extract and gall powder of Quercus brantii Lindl. and reached close to normal levels.The level of total phenols (GAE/100 g of sample) and free gallic acid were estimated to be 88.43 ±7.23 (mean ± SD) and 3.74% of dry weight, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, and Medicinal Plants Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

ABSTRACT

Introduction: In this study we investigated the effect of gall of Quercus brantii Lindl., a traditional Iranian medicine, in a murine model of experimental colitis induced in male rats by rectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS).

Material and methods: Quantification of the main active components was done for estimation of total phenolic content and free gallic acid. Gall of Quercus brantii Lindl. in two forms (gall powder and gall hydro alcoholic extract) was gavaged for 10 days (500 mg/kg). Ten days after induction of colitis, colonic status was examined by macroscopic, microscopic and biochemical analyses. Colonic tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were analyzed as biomarkers of inflammatory condition. To determine the role of oxidative stress (OS) in colitis, the levels of cellular lipid peroxidation (LPO), total antioxidant power (TAP) and myeloperoxidase (MPO) were measured in colon tissues.

Results: TNBS-induced colitis exhibited a significant increase in colon MPO activity and concentrations of cellular LPO, TNF-α and IL-1β, while TAP was significantly reduced. Microscopic evaluations of the colonic damage in the colitis group revealed multifocal degenerative changes in the epithelial lining and areas of necrosis, extensive mucosal and sub-mucosal damage with congested blood vessels, edema and hemorrhages along with extensive infiltration of inflammatory cells. Parameters including macroscopic and microscopic scores, TNF-α, IL-1β, LPO, TAP and MPO improved by both gall extract and gall powder of Quercus brantii Lindl. and reached close to normal levels. The level of total phenols (GAE/100 g of sample) and free gallic acid were estimated to be 88.43 ±7.23 (mean ± SD) and 3.74% of dry weight, respectively.

Conclusions: The present study indicates that the gall of Quercus brantii Lindl. is able to exert antioxidative and anti-inflammatory effects on the biochemical and pathological parameters of colitis.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph of colon trans-mural histology in sham and experimental groups. H + E: hematoxylin and eosin stain. Original magnification: 100×. A – No histological modification is present in the sham animals and a regular mucosal layer (a) with intact epithelial surface (b) is seen. In the colitis group (B, C and D), multifocal degeneration and necrosis of epithelium (c, d), massive infiltration of inflammatory cells in the mucosa and sub-mucosa (e), obvious hemorrhages and congestion (f), edema (g), and crypt abscesses (h) are observed. Treatment with gall powder (E) reduced the morphological alteration showing protection of mucosal and epithelium architecture (i) with some focal areas of epithelial necrosis and mild inflammatory cell infiltration. No abnormality is seen in the extract-treated group (F). Treatment with Quercus brantii completely protected the morphological alteration associated with TNBS administration. Note the intact mucosal layer (j) and epithelial surface (k)
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Figure 0002: Photomicrograph of colon trans-mural histology in sham and experimental groups. H + E: hematoxylin and eosin stain. Original magnification: 100×. A – No histological modification is present in the sham animals and a regular mucosal layer (a) with intact epithelial surface (b) is seen. In the colitis group (B, C and D), multifocal degeneration and necrosis of epithelium (c, d), massive infiltration of inflammatory cells in the mucosa and sub-mucosa (e), obvious hemorrhages and congestion (f), edema (g), and crypt abscesses (h) are observed. Treatment with gall powder (E) reduced the morphological alteration showing protection of mucosal and epithelium architecture (i) with some focal areas of epithelial necrosis and mild inflammatory cell infiltration. No abnormality is seen in the extract-treated group (F). Treatment with Quercus brantii completely protected the morphological alteration associated with TNBS administration. Note the intact mucosal layer (j) and epithelial surface (k)

Mentions: Light microscopic examination of the colon in the sham group showed normal histology and revealed a regular mucosal layer with intact epithelial surface (Figure 2A).


On the benefit of galls of Quercus brantii Lindl. in murine colitis: the role of free gallic acid.

Khanavi M, Sabbagh-Bani-Azad M, Abdolghaffari AH, Vazirian M, Isazadeh I, Rezvanfar MA, Baeeri M, Mohammadirad A, Rahimi R, Shams-Ardekani MR, Abdollahi M - Arch Med Sci (2014)

Photomicrograph of colon trans-mural histology in sham and experimental groups. H + E: hematoxylin and eosin stain. Original magnification: 100×. A – No histological modification is present in the sham animals and a regular mucosal layer (a) with intact epithelial surface (b) is seen. In the colitis group (B, C and D), multifocal degeneration and necrosis of epithelium (c, d), massive infiltration of inflammatory cells in the mucosa and sub-mucosa (e), obvious hemorrhages and congestion (f), edema (g), and crypt abscesses (h) are observed. Treatment with gall powder (E) reduced the morphological alteration showing protection of mucosal and epithelium architecture (i) with some focal areas of epithelial necrosis and mild inflammatory cell infiltration. No abnormality is seen in the extract-treated group (F). Treatment with Quercus brantii completely protected the morphological alteration associated with TNBS administration. Note the intact mucosal layer (j) and epithelial surface (k)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4296077&req=5

Figure 0002: Photomicrograph of colon trans-mural histology in sham and experimental groups. H + E: hematoxylin and eosin stain. Original magnification: 100×. A – No histological modification is present in the sham animals and a regular mucosal layer (a) with intact epithelial surface (b) is seen. In the colitis group (B, C and D), multifocal degeneration and necrosis of epithelium (c, d), massive infiltration of inflammatory cells in the mucosa and sub-mucosa (e), obvious hemorrhages and congestion (f), edema (g), and crypt abscesses (h) are observed. Treatment with gall powder (E) reduced the morphological alteration showing protection of mucosal and epithelium architecture (i) with some focal areas of epithelial necrosis and mild inflammatory cell infiltration. No abnormality is seen in the extract-treated group (F). Treatment with Quercus brantii completely protected the morphological alteration associated with TNBS administration. Note the intact mucosal layer (j) and epithelial surface (k)
Mentions: Light microscopic examination of the colon in the sham group showed normal histology and revealed a regular mucosal layer with intact epithelial surface (Figure 2A).

Bottom Line: TNBS-induced colitis exhibited a significant increase in colon MPO activity and concentrations of cellular LPO, TNF-α and IL-1β, while TAP was significantly reduced.Parameters including macroscopic and microscopic scores, TNF-α, IL-1β, LPO, TAP and MPO improved by both gall extract and gall powder of Quercus brantii Lindl. and reached close to normal levels.The level of total phenols (GAE/100 g of sample) and free gallic acid were estimated to be 88.43 ±7.23 (mean ± SD) and 3.74% of dry weight, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, and Medicinal Plants Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

ABSTRACT

Introduction: In this study we investigated the effect of gall of Quercus brantii Lindl., a traditional Iranian medicine, in a murine model of experimental colitis induced in male rats by rectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS).

Material and methods: Quantification of the main active components was done for estimation of total phenolic content and free gallic acid. Gall of Quercus brantii Lindl. in two forms (gall powder and gall hydro alcoholic extract) was gavaged for 10 days (500 mg/kg). Ten days after induction of colitis, colonic status was examined by macroscopic, microscopic and biochemical analyses. Colonic tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were analyzed as biomarkers of inflammatory condition. To determine the role of oxidative stress (OS) in colitis, the levels of cellular lipid peroxidation (LPO), total antioxidant power (TAP) and myeloperoxidase (MPO) were measured in colon tissues.

Results: TNBS-induced colitis exhibited a significant increase in colon MPO activity and concentrations of cellular LPO, TNF-α and IL-1β, while TAP was significantly reduced. Microscopic evaluations of the colonic damage in the colitis group revealed multifocal degenerative changes in the epithelial lining and areas of necrosis, extensive mucosal and sub-mucosal damage with congested blood vessels, edema and hemorrhages along with extensive infiltration of inflammatory cells. Parameters including macroscopic and microscopic scores, TNF-α, IL-1β, LPO, TAP and MPO improved by both gall extract and gall powder of Quercus brantii Lindl. and reached close to normal levels. The level of total phenols (GAE/100 g of sample) and free gallic acid were estimated to be 88.43 ±7.23 (mean ± SD) and 3.74% of dry weight, respectively.

Conclusions: The present study indicates that the gall of Quercus brantii Lindl. is able to exert antioxidative and anti-inflammatory effects on the biochemical and pathological parameters of colitis.

No MeSH data available.


Related in: MedlinePlus