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Streptozotocin diabetes attenuates the effects of nondepolarizing neuromuscular relaxants on rat muscles.

Huang L, Chen D, Li S - Korean J. Physiol. Pharmacol. (2014)

Bottom Line: The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups.For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group.For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, The Affiliated First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.

ABSTRACT
The hypothesis of this study was that diabetes-induced desensitization of rat soleus (SOL) and extensor digitorum longus (EDL) to non-depolarizing muscle relaxants (NDMRs) depends on the stage of diabetes and on the kind of NDMRs. We tested the different magnitude of resistance to vecuronium, cisatracurium, and rocuronium at different stages of streptozotocin (STZ)-induced diabetes by the EDL sciatic nerve-muscle preparations, and the SOL sciatic nerve-muscle preparations from rats after 4 and 16 weeks of STZ treatment. The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups. Concentration giving 50% of maximal inhibition (IC50) was larger in the diabetic groups for all the NDMRs. For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group. For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group. Diabetes-induced desensitization to NDMRs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of NDMRs. The resistance to NDMRs was stronger in the later stage of diabetes (16 versus 4 weeks after STZ treatment). Additionally, when monitoring in SOL, diabetes attenuated the actions of neuromuscular blockade more intensely than that in EDL. Nonetheless, the hyposensitivity to NDMRs in diabetes was not relevant for the kind of NDMRs.

No MeSH data available.


Related in: MedlinePlus

Concentration-twitch tension curves for rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F). The twitch tension was elicited by indirect stimulation at 0.1 Hz. Values are expressed as mean±SD (n=10 each). The concentration-twitch tension curves of these groups were significantly different for each NDMRs compared with that of control value (p<0.05, data not presented here). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F), there was a significantly difference between the curves in the dia 4 wk or dia16wk group and those in control group (p<0.05). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (F), there was a significantly difference between the curves in the dia4wk group and those in dia 16 wk group (p<0.05). But there was not significantly difference between the curves in the dia 4 wk group and those in dia 16 wk group for cisatracurium in EDL (p>0.05). *p<0.05 versus con group, &p<0.05 versus dia 4 wk group. EDL, Extensor digitorum longus; SOL, Soleus; con, control group; dia 4 wk, diabetic 4 weeks group; dia 16 wk, diabetic 4 weeks group.
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Figure 3: Concentration-twitch tension curves for rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F). The twitch tension was elicited by indirect stimulation at 0.1 Hz. Values are expressed as mean±SD (n=10 each). The concentration-twitch tension curves of these groups were significantly different for each NDMRs compared with that of control value (p<0.05, data not presented here). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F), there was a significantly difference between the curves in the dia 4 wk or dia16wk group and those in control group (p<0.05). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (F), there was a significantly difference between the curves in the dia4wk group and those in dia 16 wk group (p<0.05). But there was not significantly difference between the curves in the dia 4 wk group and those in dia 16 wk group for cisatracurium in EDL (p>0.05). *p<0.05 versus con group, &p<0.05 versus dia 4 wk group. EDL, Extensor digitorum longus; SOL, Soleus; con, control group; dia 4 wk, diabetic 4 weeks group; dia 16 wk, diabetic 4 weeks group.

Mentions: In all the groups, rocuronium, vecuronium and cisatracurium reduced the magnitudes of indirectly elicited twitch tensions dose-dependently (Fig. 2 and 3). For EDL and SOL in the control group, the concentration-twitch tension curves of rocuronium, vecuronium, and cisatracurium were significantly different (p<0.05; Fig. 2). Compared with vecuronium and cisatracurium, the curves were significantly shifted from rocuronium to the right in both EDL and SOL in the control group. For vecuronium in the control group, the curves were significantly shifted from cisatracurium to the right in SOL. IC50, was significantly largest for rocuronium, second largest for vecuronium, and smallest for cisatracurium in two muscles in the control group (p<0.05; Table 1). The slope for rocuronium was significantly smaller than those for vecuronium and cisatracurium in the control rats (p<0.05; Table 1). For every NDMRs, IC50 was significantly larger in EDL than in SOL in the control group. To be mentioned, the concentration-twitch tension curves and IC50 of con 16 wk group were quite the same as those of con 4 wk group, so only the data of con 4 wk group was presented in this report.


Streptozotocin diabetes attenuates the effects of nondepolarizing neuromuscular relaxants on rat muscles.

Huang L, Chen D, Li S - Korean J. Physiol. Pharmacol. (2014)

Concentration-twitch tension curves for rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F). The twitch tension was elicited by indirect stimulation at 0.1 Hz. Values are expressed as mean±SD (n=10 each). The concentration-twitch tension curves of these groups were significantly different for each NDMRs compared with that of control value (p<0.05, data not presented here). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F), there was a significantly difference between the curves in the dia 4 wk or dia16wk group and those in control group (p<0.05). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (F), there was a significantly difference between the curves in the dia4wk group and those in dia 16 wk group (p<0.05). But there was not significantly difference between the curves in the dia 4 wk group and those in dia 16 wk group for cisatracurium in EDL (p>0.05). *p<0.05 versus con group, &p<0.05 versus dia 4 wk group. EDL, Extensor digitorum longus; SOL, Soleus; con, control group; dia 4 wk, diabetic 4 weeks group; dia 16 wk, diabetic 4 weeks group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4296034&req=5

Figure 3: Concentration-twitch tension curves for rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F). The twitch tension was elicited by indirect stimulation at 0.1 Hz. Values are expressed as mean±SD (n=10 each). The concentration-twitch tension curves of these groups were significantly different for each NDMRs compared with that of control value (p<0.05, data not presented here). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (C, F), there was a significantly difference between the curves in the dia 4 wk or dia16wk group and those in control group (p<0.05). For rocuronium (A, D), vecuronium (B, E), and cisatracurium (F), there was a significantly difference between the curves in the dia4wk group and those in dia 16 wk group (p<0.05). But there was not significantly difference between the curves in the dia 4 wk group and those in dia 16 wk group for cisatracurium in EDL (p>0.05). *p<0.05 versus con group, &p<0.05 versus dia 4 wk group. EDL, Extensor digitorum longus; SOL, Soleus; con, control group; dia 4 wk, diabetic 4 weeks group; dia 16 wk, diabetic 4 weeks group.
Mentions: In all the groups, rocuronium, vecuronium and cisatracurium reduced the magnitudes of indirectly elicited twitch tensions dose-dependently (Fig. 2 and 3). For EDL and SOL in the control group, the concentration-twitch tension curves of rocuronium, vecuronium, and cisatracurium were significantly different (p<0.05; Fig. 2). Compared with vecuronium and cisatracurium, the curves were significantly shifted from rocuronium to the right in both EDL and SOL in the control group. For vecuronium in the control group, the curves were significantly shifted from cisatracurium to the right in SOL. IC50, was significantly largest for rocuronium, second largest for vecuronium, and smallest for cisatracurium in two muscles in the control group (p<0.05; Table 1). The slope for rocuronium was significantly smaller than those for vecuronium and cisatracurium in the control rats (p<0.05; Table 1). For every NDMRs, IC50 was significantly larger in EDL than in SOL in the control group. To be mentioned, the concentration-twitch tension curves and IC50 of con 16 wk group were quite the same as those of con 4 wk group, so only the data of con 4 wk group was presented in this report.

Bottom Line: The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups.For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group.For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, The Affiliated First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.

ABSTRACT
The hypothesis of this study was that diabetes-induced desensitization of rat soleus (SOL) and extensor digitorum longus (EDL) to non-depolarizing muscle relaxants (NDMRs) depends on the stage of diabetes and on the kind of NDMRs. We tested the different magnitude of resistance to vecuronium, cisatracurium, and rocuronium at different stages of streptozotocin (STZ)-induced diabetes by the EDL sciatic nerve-muscle preparations, and the SOL sciatic nerve-muscle preparations from rats after 4 and 16 weeks of STZ treatment. The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups. Concentration giving 50% of maximal inhibition (IC50) was larger in the diabetic groups for all the NDMRs. For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group. For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group. Diabetes-induced desensitization to NDMRs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of NDMRs. The resistance to NDMRs was stronger in the later stage of diabetes (16 versus 4 weeks after STZ treatment). Additionally, when monitoring in SOL, diabetes attenuated the actions of neuromuscular blockade more intensely than that in EDL. Nonetheless, the hyposensitivity to NDMRs in diabetes was not relevant for the kind of NDMRs.

No MeSH data available.


Related in: MedlinePlus