Limits...
Streptozotocin diabetes attenuates the effects of nondepolarizing neuromuscular relaxants on rat muscles.

Huang L, Chen D, Li S - Korean J. Physiol. Pharmacol. (2014)

Bottom Line: The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups.For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group.For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, The Affiliated First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.

ABSTRACT
The hypothesis of this study was that diabetes-induced desensitization of rat soleus (SOL) and extensor digitorum longus (EDL) to non-depolarizing muscle relaxants (NDMRs) depends on the stage of diabetes and on the kind of NDMRs. We tested the different magnitude of resistance to vecuronium, cisatracurium, and rocuronium at different stages of streptozotocin (STZ)-induced diabetes by the EDL sciatic nerve-muscle preparations, and the SOL sciatic nerve-muscle preparations from rats after 4 and 16 weeks of STZ treatment. The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups. Concentration giving 50% of maximal inhibition (IC50) was larger in the diabetic groups for all the NDMRs. For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group. For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group. Diabetes-induced desensitization to NDMRs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of NDMRs. The resistance to NDMRs was stronger in the later stage of diabetes (16 versus 4 weeks after STZ treatment). Additionally, when monitoring in SOL, diabetes attenuated the actions of neuromuscular blockade more intensely than that in EDL. Nonetheless, the hyposensitivity to NDMRs in diabetes was not relevant for the kind of NDMRs.

No MeSH data available.


Related in: MedlinePlus

Twitch tension height elicited by indirect stimulation in Extensor digitorum longus (EDL, A) and Soleus (SOL, B). There was a significant difference between diabetic groups and the age-matched control groups (p<0.05), and between dia 4 wk and dia 16 wk groups (p<0.05), while no significant difference between con 4 wk group and con 16 wk group. *p<0.05 versus control groups, &p<0.05 versus diabetic 4 weeks group. con 4 wk, control 4 weeks group; dia 4 wk, diabetic 4 weeks group; con 16 wk, control 16 weeks group; dia 16 wk, diabetic 16 weeks group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4296034&req=5

Figure 1: Twitch tension height elicited by indirect stimulation in Extensor digitorum longus (EDL, A) and Soleus (SOL, B). There was a significant difference between diabetic groups and the age-matched control groups (p<0.05), and between dia 4 wk and dia 16 wk groups (p<0.05), while no significant difference between con 4 wk group and con 16 wk group. *p<0.05 versus control groups, &p<0.05 versus diabetic 4 weeks group. con 4 wk, control 4 weeks group; dia 4 wk, diabetic 4 weeks group; con 16 wk, control 16 weeks group; dia 16 wk, diabetic 16 weeks group.

Mentions: The nerve-Muscle preparations (n=10 each group) were established for indirectly electrical stimulation. After rinsing the muscle strips that had been exposed to NDMR with modified Krebs solutions, the indirectly elicited twitch tension returned to 95~105% of the initial value in each study. There were significant differences in the twitch tensions between diabetic groups and the age-matched control groups (Fig. 1; p<0.05), and between dia 4 wk and dia 16 wk groups (Fig. 1; p<0.05), while there was no significant difference between con 4 wk group and con 16 wk group (Fig. 1; p>0.05).


Streptozotocin diabetes attenuates the effects of nondepolarizing neuromuscular relaxants on rat muscles.

Huang L, Chen D, Li S - Korean J. Physiol. Pharmacol. (2014)

Twitch tension height elicited by indirect stimulation in Extensor digitorum longus (EDL, A) and Soleus (SOL, B). There was a significant difference between diabetic groups and the age-matched control groups (p<0.05), and between dia 4 wk and dia 16 wk groups (p<0.05), while no significant difference between con 4 wk group and con 16 wk group. *p<0.05 versus control groups, &p<0.05 versus diabetic 4 weeks group. con 4 wk, control 4 weeks group; dia 4 wk, diabetic 4 weeks group; con 16 wk, control 16 weeks group; dia 16 wk, diabetic 16 weeks group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4296034&req=5

Figure 1: Twitch tension height elicited by indirect stimulation in Extensor digitorum longus (EDL, A) and Soleus (SOL, B). There was a significant difference between diabetic groups and the age-matched control groups (p<0.05), and between dia 4 wk and dia 16 wk groups (p<0.05), while no significant difference between con 4 wk group and con 16 wk group. *p<0.05 versus control groups, &p<0.05 versus diabetic 4 weeks group. con 4 wk, control 4 weeks group; dia 4 wk, diabetic 4 weeks group; con 16 wk, control 16 weeks group; dia 16 wk, diabetic 16 weeks group.
Mentions: The nerve-Muscle preparations (n=10 each group) were established for indirectly electrical stimulation. After rinsing the muscle strips that had been exposed to NDMR with modified Krebs solutions, the indirectly elicited twitch tension returned to 95~105% of the initial value in each study. There were significant differences in the twitch tensions between diabetic groups and the age-matched control groups (Fig. 1; p<0.05), and between dia 4 wk and dia 16 wk groups (Fig. 1; p<0.05), while there was no significant difference between con 4 wk group and con 16 wk group (Fig. 1; p>0.05).

Bottom Line: The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups.For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group.For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, The Affiliated First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.

ABSTRACT
The hypothesis of this study was that diabetes-induced desensitization of rat soleus (SOL) and extensor digitorum longus (EDL) to non-depolarizing muscle relaxants (NDMRs) depends on the stage of diabetes and on the kind of NDMRs. We tested the different magnitude of resistance to vecuronium, cisatracurium, and rocuronium at different stages of streptozotocin (STZ)-induced diabetes by the EDL sciatic nerve-muscle preparations, and the SOL sciatic nerve-muscle preparations from rats after 4 and 16 weeks of STZ treatment. The concentration-twitch tension curves were significantly shifted from those of the control group to the right in the diabetic groups. Concentration giving 50% of maximal inhibition (IC50) was larger in the diabetic groups for all the NDMRs. For rocuronium and cisatracurium in both SOL and EDL, IC50 was significantly larger in diabetic 16 weeks group than those in the diabetic 4 weeks group. For SOL/EDL, the IC50 ratios were significantly largest in the diabetic 16 weeks group, second largest in the diabetic 4 weeks group, and smallest for the control group. Diabetes-induced desensitization to NDMRs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of NDMRs. The resistance to NDMRs was stronger in the later stage of diabetes (16 versus 4 weeks after STZ treatment). Additionally, when monitoring in SOL, diabetes attenuated the actions of neuromuscular blockade more intensely than that in EDL. Nonetheless, the hyposensitivity to NDMRs in diabetes was not relevant for the kind of NDMRs.

No MeSH data available.


Related in: MedlinePlus