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Synergistic effects of NOD1 or NOD2 and TLR4 activation on mouse sickness behavior in relation to immune and brain activity markers.

Farzi A, Reichmann F, Meinitzer A, Mayerhofer R, Jain P, Hassan AM, Fröhlich EE, Wagner K, Painsipp E, Rinner B, Holzer P - Brain Behav. Immun. (2014)

Bottom Line: Intraperitoneal injection of FK565 (0.001 or 0.003mg/kg) or MDP (1 or 3mg/kg) 4h before LPS (0.1 or 0.83mg/kg) significantly aggravated and prolonged the LPS-evoked sickness behavior as deduced from a decrease in locomotion, exploration, food intake and temperature.When given alone, FK565 and MDP had only minor effects.Immunohistochemical visualization of c-Fos in the brain revealed that NOD2 synergism with TLR4 resulted in increased activation of cerebral nuclei relevant to sickness.

View Article: PubMed Central - PubMed

Affiliation: Research Unit of Translational Neurogastroenterology, Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitätsplatz 4, 8010 Graz, Austria. Electronic address: aitak.farzi@medunigraz.at.

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Representative micrographs of forebrain regions illustrating c-Fos immunoreactivity induced by MDP (3 mg/kg), LPS (0.83 mg/kg) and MDP + LPS in male mice. The left column panels show micrographs of forebrain regions taken from saline (VEH)-treated mice euthanized 3 h after injection. The second column panels depict micrographs of the same brain regions taken from MDP-treated mice, while the third column panels show micrographs of LPS-treated mice. The right column panels represent c-Fos immunolabeling induced by MDP + LPS 3 h after treatment. The squares in the left column represent the position and size of the ROIs. Abbreviations: BNSTd/v = bed nucleus of the stria terminalis dorsal/ventral, CeA = central amygdala, DG = dentate gyrus, PVN = paraventricular nucleus of the hypothalamus, SFO = subfornical organ, SO = supraoptic nucleus.
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f0045: Representative micrographs of forebrain regions illustrating c-Fos immunoreactivity induced by MDP (3 mg/kg), LPS (0.83 mg/kg) and MDP + LPS in male mice. The left column panels show micrographs of forebrain regions taken from saline (VEH)-treated mice euthanized 3 h after injection. The second column panels depict micrographs of the same brain regions taken from MDP-treated mice, while the third column panels show micrographs of LPS-treated mice. The right column panels represent c-Fos immunolabeling induced by MDP + LPS 3 h after treatment. The squares in the left column represent the position and size of the ROIs. Abbreviations: BNSTd/v = bed nucleus of the stria terminalis dorsal/ventral, CeA = central amygdala, DG = dentate gyrus, PVN = paraventricular nucleus of the hypothalamus, SFO = subfornical organ, SO = supraoptic nucleus.

Mentions: Representative micrographs showing the effects of MDP, LPS and MDP + LPS on the expression of c-Fos in the cerebral areas under study are shown in Fig. 9.


Synergistic effects of NOD1 or NOD2 and TLR4 activation on mouse sickness behavior in relation to immune and brain activity markers.

Farzi A, Reichmann F, Meinitzer A, Mayerhofer R, Jain P, Hassan AM, Fröhlich EE, Wagner K, Painsipp E, Rinner B, Holzer P - Brain Behav. Immun. (2014)

Representative micrographs of forebrain regions illustrating c-Fos immunoreactivity induced by MDP (3 mg/kg), LPS (0.83 mg/kg) and MDP + LPS in male mice. The left column panels show micrographs of forebrain regions taken from saline (VEH)-treated mice euthanized 3 h after injection. The second column panels depict micrographs of the same brain regions taken from MDP-treated mice, while the third column panels show micrographs of LPS-treated mice. The right column panels represent c-Fos immunolabeling induced by MDP + LPS 3 h after treatment. The squares in the left column represent the position and size of the ROIs. Abbreviations: BNSTd/v = bed nucleus of the stria terminalis dorsal/ventral, CeA = central amygdala, DG = dentate gyrus, PVN = paraventricular nucleus of the hypothalamus, SFO = subfornical organ, SO = supraoptic nucleus.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4295938&req=5

f0045: Representative micrographs of forebrain regions illustrating c-Fos immunoreactivity induced by MDP (3 mg/kg), LPS (0.83 mg/kg) and MDP + LPS in male mice. The left column panels show micrographs of forebrain regions taken from saline (VEH)-treated mice euthanized 3 h after injection. The second column panels depict micrographs of the same brain regions taken from MDP-treated mice, while the third column panels show micrographs of LPS-treated mice. The right column panels represent c-Fos immunolabeling induced by MDP + LPS 3 h after treatment. The squares in the left column represent the position and size of the ROIs. Abbreviations: BNSTd/v = bed nucleus of the stria terminalis dorsal/ventral, CeA = central amygdala, DG = dentate gyrus, PVN = paraventricular nucleus of the hypothalamus, SFO = subfornical organ, SO = supraoptic nucleus.
Mentions: Representative micrographs showing the effects of MDP, LPS and MDP + LPS on the expression of c-Fos in the cerebral areas under study are shown in Fig. 9.

Bottom Line: Intraperitoneal injection of FK565 (0.001 or 0.003mg/kg) or MDP (1 or 3mg/kg) 4h before LPS (0.1 or 0.83mg/kg) significantly aggravated and prolonged the LPS-evoked sickness behavior as deduced from a decrease in locomotion, exploration, food intake and temperature.When given alone, FK565 and MDP had only minor effects.Immunohistochemical visualization of c-Fos in the brain revealed that NOD2 synergism with TLR4 resulted in increased activation of cerebral nuclei relevant to sickness.

View Article: PubMed Central - PubMed

Affiliation: Research Unit of Translational Neurogastroenterology, Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitätsplatz 4, 8010 Graz, Austria. Electronic address: aitak.farzi@medunigraz.at.

Show MeSH
Related in: MedlinePlus