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Bartonella spp. bacteremia in blood donors from Campinas, Brazil.

Pitassi LH, de Paiva Diniz PP, Scorpio DG, Drummond MR, Lania BG, Barjas-Castro ML, Gilioli R, Colombo S, Sowy S, Breitschwerdt EB, Nicholson WL, Velho PE - PLoS Negl Trop Dis (2015)

Bottom Line: Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively.Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America.Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

View Article: PubMed Central - PubMed

Affiliation: Division of Dermatology, Department of Medicine, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.

ABSTRACT
Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

No MeSH data available.


Related in: MedlinePlus

Flowchart of the culture and PCR-based procedures performed to determine Bartonella prevalence in 500 blood donors from Campinas, Brazil.
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pntd.0003467.g001: Flowchart of the culture and PCR-based procedures performed to determine Bartonella prevalence in 500 blood donors from Campinas, Brazil.

Mentions: A cross-sectional study was conducted at the UNICAMP Blood Bank (HEMOCENTRO), which serves a geographic region with an estimated population of 6.4 million people in the São Paulo state, Brazil. Healthy blood donors were randomly recruited from November 19th to December 23rd 2009, at the time of their voluntary blood donation. Sample size was estimated in 473 subjects to allow for estimation of at least 5% prevalence of bloodstream infection with Bartonella spp. in blood donors, with a desired precision of 5% given a 95% confidence limit and a design effect of 1. Therefore, with possible attrition, we enrolled 500 donors. This study was approved by the Research Ethics Committee of the University of Campinas (UNICAMP), Brazil (CEP122/2005). An informed written consent, approved by the UNICAMP Research Ethics Committee, was obtained from each participant. Donor selection, blood collection, and infectious disease screening were performed in accordance with current international standards [21,22]. Following aseptic preparation of the venipuncture site and immediately after the collection of a blood unit, an additional 5 mL of whole blood was collected into a tube with ethylenediaminetetraacetic acid (EDTA) and another 5 mL was collected into a serum separator tube via the accessory port. Samples were stored at −20°C until analysis at the University of Campinas and subsequently at Western University of Health Sciences. An overview of the diagnostic procedures performed in this study is presented in the Fig. 1.


Bartonella spp. bacteremia in blood donors from Campinas, Brazil.

Pitassi LH, de Paiva Diniz PP, Scorpio DG, Drummond MR, Lania BG, Barjas-Castro ML, Gilioli R, Colombo S, Sowy S, Breitschwerdt EB, Nicholson WL, Velho PE - PLoS Negl Trop Dis (2015)

Flowchart of the culture and PCR-based procedures performed to determine Bartonella prevalence in 500 blood donors from Campinas, Brazil.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4295888&req=5

pntd.0003467.g001: Flowchart of the culture and PCR-based procedures performed to determine Bartonella prevalence in 500 blood donors from Campinas, Brazil.
Mentions: A cross-sectional study was conducted at the UNICAMP Blood Bank (HEMOCENTRO), which serves a geographic region with an estimated population of 6.4 million people in the São Paulo state, Brazil. Healthy blood donors were randomly recruited from November 19th to December 23rd 2009, at the time of their voluntary blood donation. Sample size was estimated in 473 subjects to allow for estimation of at least 5% prevalence of bloodstream infection with Bartonella spp. in blood donors, with a desired precision of 5% given a 95% confidence limit and a design effect of 1. Therefore, with possible attrition, we enrolled 500 donors. This study was approved by the Research Ethics Committee of the University of Campinas (UNICAMP), Brazil (CEP122/2005). An informed written consent, approved by the UNICAMP Research Ethics Committee, was obtained from each participant. Donor selection, blood collection, and infectious disease screening were performed in accordance with current international standards [21,22]. Following aseptic preparation of the venipuncture site and immediately after the collection of a blood unit, an additional 5 mL of whole blood was collected into a tube with ethylenediaminetetraacetic acid (EDTA) and another 5 mL was collected into a serum separator tube via the accessory port. Samples were stored at −20°C until analysis at the University of Campinas and subsequently at Western University of Health Sciences. An overview of the diagnostic procedures performed in this study is presented in the Fig. 1.

Bottom Line: Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively.Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America.Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

View Article: PubMed Central - PubMed

Affiliation: Division of Dermatology, Department of Medicine, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.

ABSTRACT
Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

No MeSH data available.


Related in: MedlinePlus