Limits...
A hormone-responsive C1-domain-containing protein At5g17960 mediates stress response in Arabidopsis thaliana.

Bhaskar RV, Mohanty B, Verma V, Wijaya E, Kumar PP - PLoS ONE (2015)

Bottom Line: At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments.Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes.Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore.

ABSTRACT
Phytohormones play a critical role in mediating plant stress response. They employ a variety of proteins for coordinating such processes. In Arabidopsis thaliana, some members of a Cys-rich protein family known as C1-clan proteins were involved in stress response, but the actual function of the protein family is largely unknown. We studied At5g17960, a C1-clan protein member that possesses three unique C1 signature domains viz. C1_2, C1_3 and ZZ/PHD type. Additionally, we identified 72 other proteins in A. thaliana that contain all three unique signature domains. Subsequently, the 73 proteins were phylogenetically classified into IX subgroups. Promoter motif analysis of the 73 genes identified the presence of hormone-responsive and stress-responsive putative cis-regulatory elements. Furthermore, we observed that transcript levels of At5g17960 were induced in response to different hormones and stress treatments. At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments. Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes. Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

Show MeSH

Related in: MedlinePlus

Response of At1g35610 and At3g13760 to GA3 treatment.The transcript levels of (A) At1g35610 and (B) At3g13760 were detected in 12-day-old WT seedlings at the indicated time points upon treatments with 10 µM GA3. The mock treatment was done using ethanol. The expression levels were normalized against TUB expression. Data presented here depict mean from three independent biological replicates with error bars representing SD. Bars with asterisks are significantly different from the mock treatments at the corresponding time points, as per Student’s t-test (P ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4295845&req=5

pone.0115418.g003: Response of At1g35610 and At3g13760 to GA3 treatment.The transcript levels of (A) At1g35610 and (B) At3g13760 were detected in 12-day-old WT seedlings at the indicated time points upon treatments with 10 µM GA3. The mock treatment was done using ethanol. The expression levels were normalized against TUB expression. Data presented here depict mean from three independent biological replicates with error bars representing SD. Bars with asterisks are significantly different from the mock treatments at the corresponding time points, as per Student’s t-test (P ≤ 0.05).

Mentions: To further verify our promoter motif analysis data, we tested two other members of subgroup IV that are closest to At5g17960 in the phylogenetic tree, viz., At1g35610 and At3g13760, for their response to GA3 treatment (Fig. 1). GA3 was selected over other phytohormones used above, owing to the relatively high motif enrichment score of GARE-like elements compared to the putative cis-elements identified for other phytohormones (Table 1). In addition, the study was initiated with the identification of At5g17960 from a GA-related microarray. Upon GA3 treatment, transcript levels of At1g35610 in 12-day-old WT seedlings depicted ~2-fold upregulation at 30 min time point, as compared to the mock treatment (Fig. 3A). However, the gene showed significant downregulation at 60 min time point, compared to the mock treatment. At3g13760 transcript levels, on the other hand, did not show any difference after 30 min, but showed a minor, yet significant downregulation at 60 min time point, upon GA3 treatment (Fig. 3B).


A hormone-responsive C1-domain-containing protein At5g17960 mediates stress response in Arabidopsis thaliana.

Bhaskar RV, Mohanty B, Verma V, Wijaya E, Kumar PP - PLoS ONE (2015)

Response of At1g35610 and At3g13760 to GA3 treatment.The transcript levels of (A) At1g35610 and (B) At3g13760 were detected in 12-day-old WT seedlings at the indicated time points upon treatments with 10 µM GA3. The mock treatment was done using ethanol. The expression levels were normalized against TUB expression. Data presented here depict mean from three independent biological replicates with error bars representing SD. Bars with asterisks are significantly different from the mock treatments at the corresponding time points, as per Student’s t-test (P ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4295845&req=5

pone.0115418.g003: Response of At1g35610 and At3g13760 to GA3 treatment.The transcript levels of (A) At1g35610 and (B) At3g13760 were detected in 12-day-old WT seedlings at the indicated time points upon treatments with 10 µM GA3. The mock treatment was done using ethanol. The expression levels were normalized against TUB expression. Data presented here depict mean from three independent biological replicates with error bars representing SD. Bars with asterisks are significantly different from the mock treatments at the corresponding time points, as per Student’s t-test (P ≤ 0.05).
Mentions: To further verify our promoter motif analysis data, we tested two other members of subgroup IV that are closest to At5g17960 in the phylogenetic tree, viz., At1g35610 and At3g13760, for their response to GA3 treatment (Fig. 1). GA3 was selected over other phytohormones used above, owing to the relatively high motif enrichment score of GARE-like elements compared to the putative cis-elements identified for other phytohormones (Table 1). In addition, the study was initiated with the identification of At5g17960 from a GA-related microarray. Upon GA3 treatment, transcript levels of At1g35610 in 12-day-old WT seedlings depicted ~2-fold upregulation at 30 min time point, as compared to the mock treatment (Fig. 3A). However, the gene showed significant downregulation at 60 min time point, compared to the mock treatment. At3g13760 transcript levels, on the other hand, did not show any difference after 30 min, but showed a minor, yet significant downregulation at 60 min time point, upon GA3 treatment (Fig. 3B).

Bottom Line: At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments.Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes.Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore.

ABSTRACT
Phytohormones play a critical role in mediating plant stress response. They employ a variety of proteins for coordinating such processes. In Arabidopsis thaliana, some members of a Cys-rich protein family known as C1-clan proteins were involved in stress response, but the actual function of the protein family is largely unknown. We studied At5g17960, a C1-clan protein member that possesses three unique C1 signature domains viz. C1_2, C1_3 and ZZ/PHD type. Additionally, we identified 72 other proteins in A. thaliana that contain all three unique signature domains. Subsequently, the 73 proteins were phylogenetically classified into IX subgroups. Promoter motif analysis of the 73 genes identified the presence of hormone-responsive and stress-responsive putative cis-regulatory elements. Furthermore, we observed that transcript levels of At5g17960 were induced in response to different hormones and stress treatments. At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments. Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes. Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

Show MeSH
Related in: MedlinePlus