Limits...
A hormone-responsive C1-domain-containing protein At5g17960 mediates stress response in Arabidopsis thaliana.

Bhaskar RV, Mohanty B, Verma V, Wijaya E, Kumar PP - PLoS ONE (2015)

Bottom Line: At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments.Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes.Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore.

ABSTRACT
Phytohormones play a critical role in mediating plant stress response. They employ a variety of proteins for coordinating such processes. In Arabidopsis thaliana, some members of a Cys-rich protein family known as C1-clan proteins were involved in stress response, but the actual function of the protein family is largely unknown. We studied At5g17960, a C1-clan protein member that possesses three unique C1 signature domains viz. C1_2, C1_3 and ZZ/PHD type. Additionally, we identified 72 other proteins in A. thaliana that contain all three unique signature domains. Subsequently, the 73 proteins were phylogenetically classified into IX subgroups. Promoter motif analysis of the 73 genes identified the presence of hormone-responsive and stress-responsive putative cis-regulatory elements. Furthermore, we observed that transcript levels of At5g17960 were induced in response to different hormones and stress treatments. At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments. Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes. Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

Show MeSH
Unrooted circular phylogenetic tree of 73 genes of C1 clan.The tree was constructed using MEGA5 with the neighbor-joining method and poisson correction of three C1-clan domains namely C1_2, C1_3 and ZZ/PHD type in Arabidopsis [17, 18]. At5g17960 is highlighted with a red box.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4295845&req=5

pone.0115418.g001: Unrooted circular phylogenetic tree of 73 genes of C1 clan.The tree was constructed using MEGA5 with the neighbor-joining method and poisson correction of three C1-clan domains namely C1_2, C1_3 and ZZ/PHD type in Arabidopsis [17, 18]. At5g17960 is highlighted with a red box.

Mentions: To study the phylogenetic relationship between 73 C1-clan proteins, a multiple sequence alignment of each of the three different C1 domains from all 73 proteins was done (S1 Fig.). An unrooted phylogenetic tree containing these 73 proteins was also generated (Fig. 1). Closely related proteins with high sequence similarity form a cluster based on common node are color-coded accordingly. Subsequently, we classified the 73 proteins of C1-clan into nine distinct subgroups based on common parental nodes shared by each subgroup. At5g17960, the protein of our interest, came under the subgroup IV along with four other proteins, namely, At1g35610, At3g13760, At3g11385 and At3g9120. Furthermore, we identified the consensus amino acid sequences of C1_2 domain (CX2CX12CX2CX8C: 29 amino acids), C1_3 domain (CX2CX11CX2CX7C: 27 amino acids) and ZZ/PHD type domain (CX2CX11CX2CX7CX28CX2C: 59 amino acids) (S2 Fig.).


A hormone-responsive C1-domain-containing protein At5g17960 mediates stress response in Arabidopsis thaliana.

Bhaskar RV, Mohanty B, Verma V, Wijaya E, Kumar PP - PLoS ONE (2015)

Unrooted circular phylogenetic tree of 73 genes of C1 clan.The tree was constructed using MEGA5 with the neighbor-joining method and poisson correction of three C1-clan domains namely C1_2, C1_3 and ZZ/PHD type in Arabidopsis [17, 18]. At5g17960 is highlighted with a red box.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4295845&req=5

pone.0115418.g001: Unrooted circular phylogenetic tree of 73 genes of C1 clan.The tree was constructed using MEGA5 with the neighbor-joining method and poisson correction of three C1-clan domains namely C1_2, C1_3 and ZZ/PHD type in Arabidopsis [17, 18]. At5g17960 is highlighted with a red box.
Mentions: To study the phylogenetic relationship between 73 C1-clan proteins, a multiple sequence alignment of each of the three different C1 domains from all 73 proteins was done (S1 Fig.). An unrooted phylogenetic tree containing these 73 proteins was also generated (Fig. 1). Closely related proteins with high sequence similarity form a cluster based on common node are color-coded accordingly. Subsequently, we classified the 73 proteins of C1-clan into nine distinct subgroups based on common parental nodes shared by each subgroup. At5g17960, the protein of our interest, came under the subgroup IV along with four other proteins, namely, At1g35610, At3g13760, At3g11385 and At3g9120. Furthermore, we identified the consensus amino acid sequences of C1_2 domain (CX2CX12CX2CX8C: 29 amino acids), C1_3 domain (CX2CX11CX2CX7C: 27 amino acids) and ZZ/PHD type domain (CX2CX11CX2CX7CX28CX2C: 59 amino acids) (S2 Fig.).

Bottom Line: At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments.Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes.Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore.

ABSTRACT
Phytohormones play a critical role in mediating plant stress response. They employ a variety of proteins for coordinating such processes. In Arabidopsis thaliana, some members of a Cys-rich protein family known as C1-clan proteins were involved in stress response, but the actual function of the protein family is largely unknown. We studied At5g17960, a C1-clan protein member that possesses three unique C1 signature domains viz. C1_2, C1_3 and ZZ/PHD type. Additionally, we identified 72 other proteins in A. thaliana that contain all three unique signature domains. Subsequently, the 73 proteins were phylogenetically classified into IX subgroups. Promoter motif analysis of the 73 genes identified the presence of hormone-responsive and stress-responsive putative cis-regulatory elements. Furthermore, we observed that transcript levels of At5g17960 were induced in response to different hormones and stress treatments. At1g35610 and At3g13760, two other members of subgroup IV, also showed upregulation upon GA3, biotic and abiotic stress treatments. Moreover, seedlings of independent transgenic A. thaliana lines ectopically expressing or suppressing At5g17960 also showed differential regulation of several abiotic stress-responsive marker genes. Thus, our data suggest that C1-domain-containing proteins have a role to play in plant hormone-mediated stress responses, thereby assigning a putative function for the C1-clan protein family.

Show MeSH