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A biomechanical characterisation of acellular porcine super flexor tendons for use in anterior cruciate ligament replacement: investigation into the effects of fat reduction and bioburden reduction bioprocesses.

Herbert A, Jones GL, Ingham E, Fisher J - J Biomech (2014)

Bottom Line: The most effective of these was then carried forward into Study 2, which investigated the use of antibiotics or peracetic acid (PAA) as a bioburden reduction agent.The most severe deviations from the profile of the native tangent modulus were found to occur in Study 2 when PAA was used for bioburden reduction.Acetone was chosen as the fat reduction step whereas, antibiotics was preferable over PAA as a bioburden reduction step.

View Article: PubMed Central - PubMed

Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.

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Related in: MedlinePlus

The mean tangent modulus (constructed using the mean values for parameters A and B) during the early phase of loading in the toe-region for the groups in (a) Study 1 and (b) Study 2. In each case the profile terminates at the transition strain (εT). ACE: acetone treatment, CM: chloroform–methanol treatment, TPAA: terminal peracetic acid treatment, PAAbio: peracetic acid bioburden treatment, ABbio: antibiotic bioburden treatment.
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f0020: The mean tangent modulus (constructed using the mean values for parameters A and B) during the early phase of loading in the toe-region for the groups in (a) Study 1 and (b) Study 2. In each case the profile terminates at the transition strain (εT). ACE: acetone treatment, CM: chloroform–methanol treatment, TPAA: terminal peracetic acid treatment, PAAbio: peracetic acid bioburden treatment, ABbio: antibiotic bioburden treatment.

Mentions: The results from the strength testing (Table 2) showed no significant variation between the groups for the clasical material paramters of UTS, E and εFAIL. Differences were, however, found to occur in the early phase of loading. Native tissue had a significantly lower transition strain (εT) compared to the tissue that had been decellularised and treated with chloroform–methanol, although the transition stress (σT) had not changed. The parameter A was significantly lower for native tissue compared to all the other decellularised tissues. No differences were found for the zero-strain tangent modulus (AB). When the mean tangent modulus in the toe-region was calculated for these groups, DC1+ACE appeared to be closest to representing the native control (Fig. 4a).


A biomechanical characterisation of acellular porcine super flexor tendons for use in anterior cruciate ligament replacement: investigation into the effects of fat reduction and bioburden reduction bioprocesses.

Herbert A, Jones GL, Ingham E, Fisher J - J Biomech (2014)

The mean tangent modulus (constructed using the mean values for parameters A and B) during the early phase of loading in the toe-region for the groups in (a) Study 1 and (b) Study 2. In each case the profile terminates at the transition strain (εT). ACE: acetone treatment, CM: chloroform–methanol treatment, TPAA: terminal peracetic acid treatment, PAAbio: peracetic acid bioburden treatment, ABbio: antibiotic bioburden treatment.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4295818&req=5

f0020: The mean tangent modulus (constructed using the mean values for parameters A and B) during the early phase of loading in the toe-region for the groups in (a) Study 1 and (b) Study 2. In each case the profile terminates at the transition strain (εT). ACE: acetone treatment, CM: chloroform–methanol treatment, TPAA: terminal peracetic acid treatment, PAAbio: peracetic acid bioburden treatment, ABbio: antibiotic bioburden treatment.
Mentions: The results from the strength testing (Table 2) showed no significant variation between the groups for the clasical material paramters of UTS, E and εFAIL. Differences were, however, found to occur in the early phase of loading. Native tissue had a significantly lower transition strain (εT) compared to the tissue that had been decellularised and treated with chloroform–methanol, although the transition stress (σT) had not changed. The parameter A was significantly lower for native tissue compared to all the other decellularised tissues. No differences were found for the zero-strain tangent modulus (AB). When the mean tangent modulus in the toe-region was calculated for these groups, DC1+ACE appeared to be closest to representing the native control (Fig. 4a).

Bottom Line: The most effective of these was then carried forward into Study 2, which investigated the use of antibiotics or peracetic acid (PAA) as a bioburden reduction agent.The most severe deviations from the profile of the native tangent modulus were found to occur in Study 2 when PAA was used for bioburden reduction.Acetone was chosen as the fat reduction step whereas, antibiotics was preferable over PAA as a bioburden reduction step.

View Article: PubMed Central - PubMed

Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.

Show MeSH
Related in: MedlinePlus