A biomechanical characterisation of acellular porcine super flexor tendons for use in anterior cruciate ligament replacement: investigation into the effects of fat reduction and bioburden reduction bioprocesses.
Bottom Line: The most effective of these was then carried forward into Study 2, which investigated the use of antibiotics or peracetic acid (PAA) as a bioburden reduction agent.The most severe deviations from the profile of the native tangent modulus were found to occur in Study 2 when PAA was used for bioburden reduction.Acetone was chosen as the fat reduction step whereas, antibiotics was preferable over PAA as a bioburden reduction step.
Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.Show MeSH
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Mentions: The results from the strength testing (Table 2) showed no significant variation between the groups for the clasical material paramters of UTS, E and εFAIL. Differences were, however, found to occur in the early phase of loading. Native tissue had a significantly lower transition strain (εT) compared to the tissue that had been decellularised and treated with chloroform–methanol, although the transition stress (σT) had not changed. The parameter A was significantly lower for native tissue compared to all the other decellularised tissues. No differences were found for the zero-strain tangent modulus (AB). When the mean tangent modulus in the toe-region was calculated for these groups, DC1+ACE appeared to be closest to representing the native control (Fig. 4a).
Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.