A biomechanical characterisation of acellular porcine super flexor tendons for use in anterior cruciate ligament replacement: investigation into the effects of fat reduction and bioburden reduction bioprocesses.
Bottom Line: The most effective of these was then carried forward into Study 2, which investigated the use of antibiotics or peracetic acid (PAA) as a bioburden reduction agent.The most severe deviations from the profile of the native tangent modulus were found to occur in Study 2 when PAA was used for bioburden reduction.Acetone was chosen as the fat reduction step whereas, antibiotics was preferable over PAA as a bioburden reduction step.
Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.Show MeSH
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Mentions: Specimens were mounted via bespoke grips (Fig. 2) to an Instron 3365 (Instron, Bucks, UK) materials testing machine equipped with a 500 N load cell. The grips were manufactured to utilise dry ice to apply the long established principles of ‘cryo-grips’ (Riemersa and Schamhardt, 1982). A probe was used to measure the temperature of the specimens at the gauge length, to ensure they had remained at room temperature and had not been adversely affected by the freezing action of the grips.
Affiliation: (IMBE) Institute of Medical and Biological Engineering, School of Mechanical Engineering University of Leeds, Leeds, UK. Electronic address: A.Herbert@leeds.ac.uk.