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Filling gaps with construction of a genetic linkage map in tetraploid roses.

Yu C, Luo L, Pan H, Guo X, Wan H, Zhang Q - Front Plant Sci (2015)

Bottom Line: The integrated linkage map was composed of 295 polymorphic markers that spanned 874 cM, and it had a mean intermarker distance of 2.9 cM.In addition, a set of newly developed EST-SSRs that are distributed evenly throughout the mapping population were released.The work identified 67 anchoring points that came from 43 common SSRs.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture, Beijing Forestry University Beijing, China.

ABSTRACT
Rose (Rosa sp.) is one of the most economically important ornamental crops worldwide. The present work contains a genetic linkage map for tetraploid roses that was constructed from an F1 segregation population using AFLPs and SSRs on 189 individuals. The preliminary 'Yunzheng Xiawei' and 'Sun City' maps consisted of 298 and 255 markers arranged into 26 and 32 linkage groups, respectively. The recombined parental maps covered 737 and 752 cM of the genome, respectively. The integrated linkage map was composed of 295 polymorphic markers that spanned 874 cM, and it had a mean intermarker distance of 2.9 cM. In addition, a set of newly developed EST-SSRs that are distributed evenly throughout the mapping population were released. The work identified 67 anchoring points that came from 43 common SSRs. The results that were produced from a large number of individuals (189) and polymorphic SSRs (242) will enhance the ability to construct higher density consensus maps with the available diploid level rose maps, and they will definitely serve as a tool for accurate QTL detection and marker assisted selection.

No MeSH data available.


Final integrated map for ‘Yunzheng Xiawei’ and ‘Sun City.’ Map distances are shown in cM on the left of each linkage group. The distorted segregating markers are underlined. The markers derived from the newly developed EST-SSRs are marked in blue. Common markers between the final integrated map and the integrated consensus map (ICM) (Spiller et al., 2011) are marked in green. Common markers with the K5 map (Koning-Boucoiran et al., 2012) are marked with a red anchoring point .
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Figure 3: Final integrated map for ‘Yunzheng Xiawei’ and ‘Sun City.’ Map distances are shown in cM on the left of each linkage group. The distorted segregating markers are underlined. The markers derived from the newly developed EST-SSRs are marked in blue. Common markers between the final integrated map and the integrated consensus map (ICM) (Spiller et al., 2011) are marked in green. Common markers with the K5 map (Koning-Boucoiran et al., 2012) are marked with a red anchoring point .

Mentions: Finally, the two maps were combined to form a single integrated map with 74 pairs of common SSRs available for both recombined parental maps. The markers on the integrated map have a similar order as to when they were on the separate parental maps. The final map was aligned with seven integrated linkage groups, which had a calculated total length of 874 cM and 295 polymorphic markers (Figure 3).


Filling gaps with construction of a genetic linkage map in tetraploid roses.

Yu C, Luo L, Pan H, Guo X, Wan H, Zhang Q - Front Plant Sci (2015)

Final integrated map for ‘Yunzheng Xiawei’ and ‘Sun City.’ Map distances are shown in cM on the left of each linkage group. The distorted segregating markers are underlined. The markers derived from the newly developed EST-SSRs are marked in blue. Common markers between the final integrated map and the integrated consensus map (ICM) (Spiller et al., 2011) are marked in green. Common markers with the K5 map (Koning-Boucoiran et al., 2012) are marked with a red anchoring point .
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4292389&req=5

Figure 3: Final integrated map for ‘Yunzheng Xiawei’ and ‘Sun City.’ Map distances are shown in cM on the left of each linkage group. The distorted segregating markers are underlined. The markers derived from the newly developed EST-SSRs are marked in blue. Common markers between the final integrated map and the integrated consensus map (ICM) (Spiller et al., 2011) are marked in green. Common markers with the K5 map (Koning-Boucoiran et al., 2012) are marked with a red anchoring point .
Mentions: Finally, the two maps were combined to form a single integrated map with 74 pairs of common SSRs available for both recombined parental maps. The markers on the integrated map have a similar order as to when they were on the separate parental maps. The final map was aligned with seven integrated linkage groups, which had a calculated total length of 874 cM and 295 polymorphic markers (Figure 3).

Bottom Line: The integrated linkage map was composed of 295 polymorphic markers that spanned 874 cM, and it had a mean intermarker distance of 2.9 cM.In addition, a set of newly developed EST-SSRs that are distributed evenly throughout the mapping population were released.The work identified 67 anchoring points that came from 43 common SSRs.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture, Beijing Forestry University Beijing, China.

ABSTRACT
Rose (Rosa sp.) is one of the most economically important ornamental crops worldwide. The present work contains a genetic linkage map for tetraploid roses that was constructed from an F1 segregation population using AFLPs and SSRs on 189 individuals. The preliminary 'Yunzheng Xiawei' and 'Sun City' maps consisted of 298 and 255 markers arranged into 26 and 32 linkage groups, respectively. The recombined parental maps covered 737 and 752 cM of the genome, respectively. The integrated linkage map was composed of 295 polymorphic markers that spanned 874 cM, and it had a mean intermarker distance of 2.9 cM. In addition, a set of newly developed EST-SSRs that are distributed evenly throughout the mapping population were released. The work identified 67 anchoring points that came from 43 common SSRs. The results that were produced from a large number of individuals (189) and polymorphic SSRs (242) will enhance the ability to construct higher density consensus maps with the available diploid level rose maps, and they will definitely serve as a tool for accurate QTL detection and marker assisted selection.

No MeSH data available.