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Estrogen receptor (α and β) but not androgen receptor expression is correlated with recurrence, progression and survival in post prostatectomy T3N0M0 locally advanced prostate cancer in an urban Greek population.

Megas G, Chrisofos M, Anastasiou I, Tsitlidou A, Choreftaki T, Deliveliotis C - Asian J. Androl. (2015 Jan-Feb)

Bottom Line: AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis.A univariate Cox regression analysis showed that ER(α) positive staining was significantly associated with a greater hazard for all outcomes.Increased ER(β) staining was significantly associated with a lower hazard for all outcomes in the univariate analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, General Hospital of Athens "G. Gennimatas", Greece.

ABSTRACT
The objective of this study was to evaluate the expression of estrogen receptors (ER(α) and ER(β)) and androgen receptors (ARs) as prognostic factors for biochemical recurrence, disease progression and survival in patients with pT3N0M0 prostate cancer (PCa) in an urban Greek population. A total of 100 consecutive patients with pT3N0M0 PCa treated with radical prostatectomy participated in the study. The mean age and follow-up were 64.2 and 6 years, respectively. The HSCORE was used for semi-quantitative analysis of the immunoreactivity of the receptors. The prognostic value of the ER(α) and ER(β) and AR was assessed in terms of recurrence, progression, and survival. AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis. A univariate Cox regression analysis showed that ER(α) positive staining was significantly associated with a greater hazard for all outcomes. Increased ER(β) staining was significantly associated with a lower hazard for all outcomes in the univariate analysis. When both ER HSCORES were used for the analysis, it was found that patients with high ER(α) or low ER(β) HSCORES compared with patients with negatively stained ER(α) and >1.7 hSCORE ER(β) had 6.03, 10.93, and 10.53 times greater hazard for biochemical disease recurrence, progression of disease and death, respectively. Multiple Cox proportional hazard analyses showed that the age, preoperative prostate specific antigen, Gleason score and ERs were independent predictors of all outcomes. ER expression is an important prognosticator after radical prostatectomy in patients with pT3N0M0 PCa. By contrast, AR expression has limited prognostic value.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis in prostate adenocarcinoma. (a) Androgen receptor (AR) expression (diaminobenzidine (DAB)), Gleason (4 + 5) score 9, HSCORE 260, scale bar = 8 µm. (b) Estrogen receptor (ER(α)) expression (DAB), Gleason (4 + 5) score 9, HSCORE 0 with sparse positive cells in cancer, scale bar = 12.5 µm. (c) ER(β) expression (DAB), Gleason (4 + 5) score 9, HSCORE 180, scale bar = 8 µm. (d) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 25 with positive staining in the stromal cells, scale bar = 8 µm. (e) AR expression (DAB), Gleason (3 + 3) score 6, HSCORE 270, scale bar = 12.5 µm. (f) ER(β) expression (DAB), Gleason (3 + 3) score 6, HSCORE 60, scale bar = 12.5 µm. (g) ΕR(α) expression (DAB), Gleason (3 + 3) score 6, HSCORE 0, scale bar = 12.5 µm. (h) AR expression (DAB), Gleason (3 + 4) score 7, HSCORE 300, scale bar = 8 µm. (i) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0 with single cell positivity, scale bar = 8 µm. (j) ER(β) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0, scale bar = 8 μm.
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Figure 1: Immunohistochemical analysis in prostate adenocarcinoma. (a) Androgen receptor (AR) expression (diaminobenzidine (DAB)), Gleason (4 + 5) score 9, HSCORE 260, scale bar = 8 µm. (b) Estrogen receptor (ER(α)) expression (DAB), Gleason (4 + 5) score 9, HSCORE 0 with sparse positive cells in cancer, scale bar = 12.5 µm. (c) ER(β) expression (DAB), Gleason (4 + 5) score 9, HSCORE 180, scale bar = 8 µm. (d) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 25 with positive staining in the stromal cells, scale bar = 8 µm. (e) AR expression (DAB), Gleason (3 + 3) score 6, HSCORE 270, scale bar = 12.5 µm. (f) ER(β) expression (DAB), Gleason (3 + 3) score 6, HSCORE 60, scale bar = 12.5 µm. (g) ΕR(α) expression (DAB), Gleason (3 + 3) score 6, HSCORE 0, scale bar = 12.5 µm. (h) AR expression (DAB), Gleason (3 + 4) score 7, HSCORE 300, scale bar = 8 µm. (i) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0 with single cell positivity, scale bar = 8 µm. (j) ER(β) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0, scale bar = 8 μm.

Mentions: The sections were incubated at room temperature for 30 min and washed with TBST solution twice for 5 min. Next, 100 μl of polymer solution Dako EnVision System/HRP (DAB) was added to each section for 45 min at room temperature, and then the sections were washed again with TBST solution twice for 5 min. The slides were immersed in DAB solution for 5 min in a dark chamber (working solution consists of 18 ml Dako Real substrate buffer, pH 7.5, and 1 ml DAB × 50). The slides were counterstained with hematoxylin (Vector H3404) and dehydrated in graded alcohol, dried and cover slipped (Figure 1a-1j).


Estrogen receptor (α and β) but not androgen receptor expression is correlated with recurrence, progression and survival in post prostatectomy T3N0M0 locally advanced prostate cancer in an urban Greek population.

Megas G, Chrisofos M, Anastasiou I, Tsitlidou A, Choreftaki T, Deliveliotis C - Asian J. Androl. (2015 Jan-Feb)

Immunohistochemical analysis in prostate adenocarcinoma. (a) Androgen receptor (AR) expression (diaminobenzidine (DAB)), Gleason (4 + 5) score 9, HSCORE 260, scale bar = 8 µm. (b) Estrogen receptor (ER(α)) expression (DAB), Gleason (4 + 5) score 9, HSCORE 0 with sparse positive cells in cancer, scale bar = 12.5 µm. (c) ER(β) expression (DAB), Gleason (4 + 5) score 9, HSCORE 180, scale bar = 8 µm. (d) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 25 with positive staining in the stromal cells, scale bar = 8 µm. (e) AR expression (DAB), Gleason (3 + 3) score 6, HSCORE 270, scale bar = 12.5 µm. (f) ER(β) expression (DAB), Gleason (3 + 3) score 6, HSCORE 60, scale bar = 12.5 µm. (g) ΕR(α) expression (DAB), Gleason (3 + 3) score 6, HSCORE 0, scale bar = 12.5 µm. (h) AR expression (DAB), Gleason (3 + 4) score 7, HSCORE 300, scale bar = 8 µm. (i) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0 with single cell positivity, scale bar = 8 µm. (j) ER(β) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0, scale bar = 8 μm.
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Figure 1: Immunohistochemical analysis in prostate adenocarcinoma. (a) Androgen receptor (AR) expression (diaminobenzidine (DAB)), Gleason (4 + 5) score 9, HSCORE 260, scale bar = 8 µm. (b) Estrogen receptor (ER(α)) expression (DAB), Gleason (4 + 5) score 9, HSCORE 0 with sparse positive cells in cancer, scale bar = 12.5 µm. (c) ER(β) expression (DAB), Gleason (4 + 5) score 9, HSCORE 180, scale bar = 8 µm. (d) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 25 with positive staining in the stromal cells, scale bar = 8 µm. (e) AR expression (DAB), Gleason (3 + 3) score 6, HSCORE 270, scale bar = 12.5 µm. (f) ER(β) expression (DAB), Gleason (3 + 3) score 6, HSCORE 60, scale bar = 12.5 µm. (g) ΕR(α) expression (DAB), Gleason (3 + 3) score 6, HSCORE 0, scale bar = 12.5 µm. (h) AR expression (DAB), Gleason (3 + 4) score 7, HSCORE 300, scale bar = 8 µm. (i) ER(α) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0 with single cell positivity, scale bar = 8 µm. (j) ER(β) expression (DAB), Gleason (3 + 4) score 7, HSCORE 0, scale bar = 8 μm.
Mentions: The sections were incubated at room temperature for 30 min and washed with TBST solution twice for 5 min. Next, 100 μl of polymer solution Dako EnVision System/HRP (DAB) was added to each section for 45 min at room temperature, and then the sections were washed again with TBST solution twice for 5 min. The slides were immersed in DAB solution for 5 min in a dark chamber (working solution consists of 18 ml Dako Real substrate buffer, pH 7.5, and 1 ml DAB × 50). The slides were counterstained with hematoxylin (Vector H3404) and dehydrated in graded alcohol, dried and cover slipped (Figure 1a-1j).

Bottom Line: AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis.A univariate Cox regression analysis showed that ER(α) positive staining was significantly associated with a greater hazard for all outcomes.Increased ER(β) staining was significantly associated with a lower hazard for all outcomes in the univariate analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, General Hospital of Athens "G. Gennimatas", Greece.

ABSTRACT
The objective of this study was to evaluate the expression of estrogen receptors (ER(α) and ER(β)) and androgen receptors (ARs) as prognostic factors for biochemical recurrence, disease progression and survival in patients with pT3N0M0 prostate cancer (PCa) in an urban Greek population. A total of 100 consecutive patients with pT3N0M0 PCa treated with radical prostatectomy participated in the study. The mean age and follow-up were 64.2 and 6 years, respectively. The HSCORE was used for semi-quantitative analysis of the immunoreactivity of the receptors. The prognostic value of the ER(α) and ER(β) and AR was assessed in terms of recurrence, progression, and survival. AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis. A univariate Cox regression analysis showed that ER(α) positive staining was significantly associated with a greater hazard for all outcomes. Increased ER(β) staining was significantly associated with a lower hazard for all outcomes in the univariate analysis. When both ER HSCORES were used for the analysis, it was found that patients with high ER(α) or low ER(β) HSCORES compared with patients with negatively stained ER(α) and >1.7 hSCORE ER(β) had 6.03, 10.93, and 10.53 times greater hazard for biochemical disease recurrence, progression of disease and death, respectively. Multiple Cox proportional hazard analyses showed that the age, preoperative prostate specific antigen, Gleason score and ERs were independent predictors of all outcomes. ER expression is an important prognosticator after radical prostatectomy in patients with pT3N0M0 PCa. By contrast, AR expression has limited prognostic value.

No MeSH data available.


Related in: MedlinePlus