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Expression of kallikrein-related peptidase 7 is decreased in prostate cancer.

Zhang CY, Zhu Y, Rui WB, Dai J, Shen ZJ - Asian J. Androl. (2015 Jan-Feb)

Bottom Line: A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001).The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue.Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

ABSTRACT
Recent evidence suggests that the human kallikrein 7 (KLK7) is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein-related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer (PCa) and 92 with benign prostate hyperplasia (BPH) were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blot, kallikrein-related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein-related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001). The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

No MeSH data available.


Related in: MedlinePlus

Western blots showing expression of hK7 in PCa tissues, BPH tissues and normal prostate tissue. BPH: benign prostate hyperplasia; PCa: prostate cancer.
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Figure 3: Western blots showing expression of hK7 in PCa tissues, BPH tissues and normal prostate tissue. BPH: benign prostate hyperplasia; PCa: prostate cancer.

Mentions: Immunohistochemical expression of KLK7 was examined in 92 BPH tissues, and 116 PCa tissues. We observed positive staining of KLK7 in BPH epithelium; however, little or no staining of KLK7 was observed in PCa (Figure 2). A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001). Western blot analyses NIL the results, and we also found the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue, and the difference between these groups had statistical significance (P < 0.001) (Figure 3).


Expression of kallikrein-related peptidase 7 is decreased in prostate cancer.

Zhang CY, Zhu Y, Rui WB, Dai J, Shen ZJ - Asian J. Androl. (2015 Jan-Feb)

Western blots showing expression of hK7 in PCa tissues, BPH tissues and normal prostate tissue. BPH: benign prostate hyperplasia; PCa: prostate cancer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4291851&req=5

Figure 3: Western blots showing expression of hK7 in PCa tissues, BPH tissues and normal prostate tissue. BPH: benign prostate hyperplasia; PCa: prostate cancer.
Mentions: Immunohistochemical expression of KLK7 was examined in 92 BPH tissues, and 116 PCa tissues. We observed positive staining of KLK7 in BPH epithelium; however, little or no staining of KLK7 was observed in PCa (Figure 2). A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001). Western blot analyses NIL the results, and we also found the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue, and the difference between these groups had statistical significance (P < 0.001) (Figure 3).

Bottom Line: A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001).The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue.Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

ABSTRACT
Recent evidence suggests that the human kallikrein 7 (KLK7) is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein-related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer (PCa) and 92 with benign prostate hyperplasia (BPH) were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blot, kallikrein-related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein-related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001). The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

No MeSH data available.


Related in: MedlinePlus