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Neutrophils are required for both the sensitization and elicitation phase of contact hypersensitivity.

Weber FC, Németh T, Csepregi JZ, Dudeck A, Roers A, Ozsvári B, Oswald E, Puskás LG, Jakob T, Mócsai A, Martin SF - J. Exp. Med. (2014)

Bottom Line: Though numerous cellular and molecular players are known, the mechanism of chemical-induced sensitization remains poorly understood.Neutrophil deficiency reduced contact allergen-induced cytokine production, gelatinase release, and reactive oxygen species production in naive mice.Lymph node cells from mice sensitized in the absence of neutrophils failed to transfer sensitization to naive recipients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Allergy Research Group, Department of Dermatology, Medical Center, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany Allergy Research Group, Department of Dermatology, Medical Center, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany Department of Physiology, Semmelweis University School of Medicine; MTA-SE "Lendület" Inflammation Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, 1094 Budapest, Hungary.

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Neutrophils in the CHS sensitization phase. (A) Analysis of the number of circulating neutrophils before or at the indicated times after treatment with the neutrophil-depleting anti-Ly6G antibody. (B and C) Infiltration of neutrophils was tested at the indicated times after sensitization with TNCB (B) or 24 h after sensitization with TNCB, DNCB, oxazolone (Oxa), or croton oil (Cr O) on the ears. Neutrophil infiltration was assessed by digestion of the ear skin followed by flow cytometry. The graphs show mean and SEM from 7–26 (A), 8–11 (B), or 9 (C) mice per group from 6 (A) or 4 (B and C) independent experiments.
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fig2: Neutrophils in the CHS sensitization phase. (A) Analysis of the number of circulating neutrophils before or at the indicated times after treatment with the neutrophil-depleting anti-Ly6G antibody. (B and C) Infiltration of neutrophils was tested at the indicated times after sensitization with TNCB (B) or 24 h after sensitization with TNCB, DNCB, oxazolone (Oxa), or croton oil (Cr O) on the ears. Neutrophil infiltration was assessed by digestion of the ear skin followed by flow cytometry. The graphs show mean and SEM from 7–26 (A), 8–11 (B), or 9 (C) mice per group from 6 (A) or 4 (B and C) independent experiments.

Mentions: We next tested the time-course of circulating neutrophil numbers after antibody-mediated neutrophil depletion. As shown in Fig. 2 A, circulating neutrophils were almost completely absent 1 d after the depletion (which corresponds to the time of sensitization in CHS experiments) and remained at very low levels for two additional days. Neutrophil numbers were normalized (P = 0.40) 6 d after depletion, which corresponds to the time of elicitation. Those results suggest that the effect of neutrophil depletion on the CHS response is caused by the absence of neutrophils during the sensitization phase, rather than a prolonged effect causing neutrophil depletion also during the elicitation phase.


Neutrophils are required for both the sensitization and elicitation phase of contact hypersensitivity.

Weber FC, Németh T, Csepregi JZ, Dudeck A, Roers A, Ozsvári B, Oswald E, Puskás LG, Jakob T, Mócsai A, Martin SF - J. Exp. Med. (2014)

Neutrophils in the CHS sensitization phase. (A) Analysis of the number of circulating neutrophils before or at the indicated times after treatment with the neutrophil-depleting anti-Ly6G antibody. (B and C) Infiltration of neutrophils was tested at the indicated times after sensitization with TNCB (B) or 24 h after sensitization with TNCB, DNCB, oxazolone (Oxa), or croton oil (Cr O) on the ears. Neutrophil infiltration was assessed by digestion of the ear skin followed by flow cytometry. The graphs show mean and SEM from 7–26 (A), 8–11 (B), or 9 (C) mice per group from 6 (A) or 4 (B and C) independent experiments.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4291534&req=5

fig2: Neutrophils in the CHS sensitization phase. (A) Analysis of the number of circulating neutrophils before or at the indicated times after treatment with the neutrophil-depleting anti-Ly6G antibody. (B and C) Infiltration of neutrophils was tested at the indicated times after sensitization with TNCB (B) or 24 h after sensitization with TNCB, DNCB, oxazolone (Oxa), or croton oil (Cr O) on the ears. Neutrophil infiltration was assessed by digestion of the ear skin followed by flow cytometry. The graphs show mean and SEM from 7–26 (A), 8–11 (B), or 9 (C) mice per group from 6 (A) or 4 (B and C) independent experiments.
Mentions: We next tested the time-course of circulating neutrophil numbers after antibody-mediated neutrophil depletion. As shown in Fig. 2 A, circulating neutrophils were almost completely absent 1 d after the depletion (which corresponds to the time of sensitization in CHS experiments) and remained at very low levels for two additional days. Neutrophil numbers were normalized (P = 0.40) 6 d after depletion, which corresponds to the time of elicitation. Those results suggest that the effect of neutrophil depletion on the CHS response is caused by the absence of neutrophils during the sensitization phase, rather than a prolonged effect causing neutrophil depletion also during the elicitation phase.

Bottom Line: Though numerous cellular and molecular players are known, the mechanism of chemical-induced sensitization remains poorly understood.Neutrophil deficiency reduced contact allergen-induced cytokine production, gelatinase release, and reactive oxygen species production in naive mice.Lymph node cells from mice sensitized in the absence of neutrophils failed to transfer sensitization to naive recipients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Allergy Research Group, Department of Dermatology, Medical Center, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany Allergy Research Group, Department of Dermatology, Medical Center, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany Department of Physiology, Semmelweis University School of Medicine; MTA-SE "Lendület" Inflammation Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, 1094 Budapest, Hungary.

Show MeSH
Related in: MedlinePlus