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Identical substitutions in magnesium chelatase paralogs result in chlorophyll-deficient soybean mutants.

Campbell BW, Mani D, Curtin SJ, Slattery RA, Michno JM, Ort DR, Schaus PJ, Palmer RG, Orf JH, Stupar RM - G3 (Bethesda) (2014)

Bottom Line: This mutation was identified in the ChlI1b gene, a paralog of ChlI1a.Protein sequence alignments of the two Mg-chelatase subunits indicated that the sites of amino acid modification in MinnGold, T219H, and CD-5 are highly conserved among photosynthetic species.These results suggest that amino acid alterations in this critical domain may create competitive inhibitory interactions between the mutant and wild-type ChlI1a and ChlI1b proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.

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Phenotypic evaluation of chlorophyll deficiency in the MinnGold mutant. (A) Visual comparison of the MinnGold mutant (left) vs. the wild-type cultivar ‘Williams 82’ (right). (B) Total leaf tissue chlorophyll levels in the MinnGold, MinnGold non-transgenic segregants, Transgenic MinnGold T1, and ‘Williams 82’.
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fig1: Phenotypic evaluation of chlorophyll deficiency in the MinnGold mutant. (A) Visual comparison of the MinnGold mutant (left) vs. the wild-type cultivar ‘Williams 82’ (right). (B) Total leaf tissue chlorophyll levels in the MinnGold, MinnGold non-transgenic segregants, Transgenic MinnGold T1, and ‘Williams 82’.

Mentions: The spontaneous, nonlethal yellow foliage mutant (Figure 1) was first observed in a segregating F3 population derived from a cross between M99-274166 × ‘MN0091’. The F3 population was advanced by modified single seed descent and exhibited a segregation ratio of 157 green (wild-type) plants to 50 yellow (mutant) individuals. The ratio of 157:50 for the wild-type:chlorophyll-deficient phenotype is consistent with a single-locus, recessive mutation (χ2 test, P = 0.78). The mutant phenotype appears more vigorous than previously identified chlorophyll-deficient soybean mutants. Measurement of leaf chlorophyll levels from tissue collected from the second true leaves at the V5 leaf stage show that this mutant displayed a dramatic reduction of both chlorophyll a and chlorophyll b relative to wild-type soybean plants (Figure 1). This mutant was given the name MinnGold because of its bright yellow leaf coloration during early foliar development.


Identical substitutions in magnesium chelatase paralogs result in chlorophyll-deficient soybean mutants.

Campbell BW, Mani D, Curtin SJ, Slattery RA, Michno JM, Ort DR, Schaus PJ, Palmer RG, Orf JH, Stupar RM - G3 (Bethesda) (2014)

Phenotypic evaluation of chlorophyll deficiency in the MinnGold mutant. (A) Visual comparison of the MinnGold mutant (left) vs. the wild-type cultivar ‘Williams 82’ (right). (B) Total leaf tissue chlorophyll levels in the MinnGold, MinnGold non-transgenic segregants, Transgenic MinnGold T1, and ‘Williams 82’.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4291463&req=5

fig1: Phenotypic evaluation of chlorophyll deficiency in the MinnGold mutant. (A) Visual comparison of the MinnGold mutant (left) vs. the wild-type cultivar ‘Williams 82’ (right). (B) Total leaf tissue chlorophyll levels in the MinnGold, MinnGold non-transgenic segregants, Transgenic MinnGold T1, and ‘Williams 82’.
Mentions: The spontaneous, nonlethal yellow foliage mutant (Figure 1) was first observed in a segregating F3 population derived from a cross between M99-274166 × ‘MN0091’. The F3 population was advanced by modified single seed descent and exhibited a segregation ratio of 157 green (wild-type) plants to 50 yellow (mutant) individuals. The ratio of 157:50 for the wild-type:chlorophyll-deficient phenotype is consistent with a single-locus, recessive mutation (χ2 test, P = 0.78). The mutant phenotype appears more vigorous than previously identified chlorophyll-deficient soybean mutants. Measurement of leaf chlorophyll levels from tissue collected from the second true leaves at the V5 leaf stage show that this mutant displayed a dramatic reduction of both chlorophyll a and chlorophyll b relative to wild-type soybean plants (Figure 1). This mutant was given the name MinnGold because of its bright yellow leaf coloration during early foliar development.

Bottom Line: This mutation was identified in the ChlI1b gene, a paralog of ChlI1a.Protein sequence alignments of the two Mg-chelatase subunits indicated that the sites of amino acid modification in MinnGold, T219H, and CD-5 are highly conserved among photosynthetic species.These results suggest that amino acid alterations in this critical domain may create competitive inhibitory interactions between the mutant and wild-type ChlI1a and ChlI1b proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.

Show MeSH