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Investigation of CPD and HMDS sample preparation techniques for cervical cells in developing computer-aided screening system based on FE-SEM/EDX.

Jusman Y, Ng SC, Abu Osman NA - ScientificWorldJournal (2014)

Bottom Line: Cervical cell image quality, elemental composition, and processing time are considered for comparison of performances.The HMDS technique has shorter processing time than the CPD technique.The results indicate that FE-SEM imaging, elemental composition, and processing time for sample preparation with the HMDS technique were better than CPD technique for cervical cell preparation technique for developing computer-aided screening system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
This paper investigated the effects of critical-point drying (CPD) and hexamethyldisilazane (HMDS) sample preparation techniques for cervical cells on field emission scanning electron microscopy and energy dispersive X-ray (FE-SEM/EDX). We investigated the visualization of cervical cell image and elemental distribution on the cervical cell for two techniques of sample preparation. Using FE-SEM/EDX, the cervical cell images are captured and the cell element compositions are extracted for both sample preparation techniques. Cervical cell image quality, elemental composition, and processing time are considered for comparison of performances. Qualitatively, FE-SEM image based on HMDS preparation technique has better image quality than CPD technique in terms of degree of spread cell on the specimen and morphologic signs of cell deteriorations (i.e., existence of plate and pellet drying artifacts and membrane blebs). Quantitatively, with mapping and line scanning EDX analysis, carbon and oxygen element compositions in HMDS technique were higher than the CPD technique in terms of weight percentages. The HMDS technique has shorter processing time than the CPD technique. The results indicate that FE-SEM imaging, elemental composition, and processing time for sample preparation with the HMDS technique were better than CPD technique for cervical cell preparation technique for developing computer-aided screening system.

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Related in: MedlinePlus

Analysis techniques for FE-SEM/EDX data based on CPD and HMDS preparation techniques.
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Related In: Results  -  Collection


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fig4: Analysis techniques for FE-SEM/EDX data based on CPD and HMDS preparation techniques.

Mentions: The analyses for comparison of a better sample preparation technique for cervical cell on FE-SEM/EDX are presented in Figure 4. The sample was subjected to FE-SEM and EDX, in order to examine its localized morphology and elemental distributions at the microscopic scale. After capturing images and scanning the specimen by FE-SEM/EDX, the outputs were presented by FE-SEM images and EDX spectrum of elemental composition in the cervical cell. Analysis for the FE-SEM images of both sample preparation techniques was compared based on their degree of spread by phase contrast microscopy at 1000–5000x magnification and morphologic signs of cell deteriorations (plate and pellet drying artifacts, membrane blebs, and cytoplasmic vacuoles) [17].


Investigation of CPD and HMDS sample preparation techniques for cervical cells in developing computer-aided screening system based on FE-SEM/EDX.

Jusman Y, Ng SC, Abu Osman NA - ScientificWorldJournal (2014)

Analysis techniques for FE-SEM/EDX data based on CPD and HMDS preparation techniques.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4291196&req=5

fig4: Analysis techniques for FE-SEM/EDX data based on CPD and HMDS preparation techniques.
Mentions: The analyses for comparison of a better sample preparation technique for cervical cell on FE-SEM/EDX are presented in Figure 4. The sample was subjected to FE-SEM and EDX, in order to examine its localized morphology and elemental distributions at the microscopic scale. After capturing images and scanning the specimen by FE-SEM/EDX, the outputs were presented by FE-SEM images and EDX spectrum of elemental composition in the cervical cell. Analysis for the FE-SEM images of both sample preparation techniques was compared based on their degree of spread by phase contrast microscopy at 1000–5000x magnification and morphologic signs of cell deteriorations (plate and pellet drying artifacts, membrane blebs, and cytoplasmic vacuoles) [17].

Bottom Line: Cervical cell image quality, elemental composition, and processing time are considered for comparison of performances.The HMDS technique has shorter processing time than the CPD technique.The results indicate that FE-SEM imaging, elemental composition, and processing time for sample preparation with the HMDS technique were better than CPD technique for cervical cell preparation technique for developing computer-aided screening system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
This paper investigated the effects of critical-point drying (CPD) and hexamethyldisilazane (HMDS) sample preparation techniques for cervical cells on field emission scanning electron microscopy and energy dispersive X-ray (FE-SEM/EDX). We investigated the visualization of cervical cell image and elemental distribution on the cervical cell for two techniques of sample preparation. Using FE-SEM/EDX, the cervical cell images are captured and the cell element compositions are extracted for both sample preparation techniques. Cervical cell image quality, elemental composition, and processing time are considered for comparison of performances. Qualitatively, FE-SEM image based on HMDS preparation technique has better image quality than CPD technique in terms of degree of spread cell on the specimen and morphologic signs of cell deteriorations (i.e., existence of plate and pellet drying artifacts and membrane blebs). Quantitatively, with mapping and line scanning EDX analysis, carbon and oxygen element compositions in HMDS technique were higher than the CPD technique in terms of weight percentages. The HMDS technique has shorter processing time than the CPD technique. The results indicate that FE-SEM imaging, elemental composition, and processing time for sample preparation with the HMDS technique were better than CPD technique for cervical cell preparation technique for developing computer-aided screening system.

Show MeSH
Related in: MedlinePlus