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A Metabonomic Study of the Effect of Methanol Extract of Ginger on Raji Cells Using (1)HNMR Spectroscopy.

Parvizzadeh N, Sadeghi S, Irani S, Iravani A, Kalayee Z, Rahimi NA, Azadi M, Zamani Z - Biotechnol Res Int (2014)

Bottom Line: Further analysis was carried out using MetaboAnalyst database.The main metabolic pathways affected by the ginger extract were detected.Ginger extract was seen to effect the protein biosynthesis, amino acid, and carbohydrate metabolism and had a strong cytotoxic effect on Raji cells in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Islamic Azad University, Science and Research Branch, Tehran 1477893855, Iran.

ABSTRACT
Cancer is currently a major international health problem. The development of resistance to chemotherapy has resulted in the search for herbal drugs. Ginger is a medicinal plant with several clinical applications. Metabolomics is a simultaneous detection of all the metabolites by use of (1)HNMR or mass spectroscopy and interpretation by modeling software. The purpose of this study was to detect the altered metabolites of Raji cells in the presence of ginger extract in vitro. Cells were cultured in the presence and absence of methanolic ginger extract in RPMI medium. IC50 determined by MTT and lipophilic and hydrophilic extracts were prepared from control and treated groups which were analyzed by (1)HNMR. The IC50 was 1000 μg/mL. Modeling of spectra was carried out on the two groups using OSC-PLS with MATLAB software and the main metabolites detected. Further analysis was carried out using MetaboAnalyst database. The main metabolic pathways affected by the ginger extract were detected. Ginger extract was seen to effect the protein biosynthesis, amino acid, and carbohydrate metabolism and had a strong cytotoxic effect on Raji cells in vitro.

No MeSH data available.


Related in: MedlinePlus

MTT assay showing effect of ginger extract on Raji cells. IC50 obtained at 0.01% dilution of stock at P < 0.05.
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fig1: MTT assay showing effect of ginger extract on Raji cells. IC50 obtained at 0.01% dilution of stock at P < 0.05.

Mentions: The 48 h effect of alcoholic ginger extract on Raji cells and percent live cells are shown in Figure 1. IC50 of Raji cells is seen at 0.01% dilution of stock which is 1000 μg/mL.


A Metabonomic Study of the Effect of Methanol Extract of Ginger on Raji Cells Using (1)HNMR Spectroscopy.

Parvizzadeh N, Sadeghi S, Irani S, Iravani A, Kalayee Z, Rahimi NA, Azadi M, Zamani Z - Biotechnol Res Int (2014)

MTT assay showing effect of ginger extract on Raji cells. IC50 obtained at 0.01% dilution of stock at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4291193&req=5

fig1: MTT assay showing effect of ginger extract on Raji cells. IC50 obtained at 0.01% dilution of stock at P < 0.05.
Mentions: The 48 h effect of alcoholic ginger extract on Raji cells and percent live cells are shown in Figure 1. IC50 of Raji cells is seen at 0.01% dilution of stock which is 1000 μg/mL.

Bottom Line: Further analysis was carried out using MetaboAnalyst database.The main metabolic pathways affected by the ginger extract were detected.Ginger extract was seen to effect the protein biosynthesis, amino acid, and carbohydrate metabolism and had a strong cytotoxic effect on Raji cells in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Islamic Azad University, Science and Research Branch, Tehran 1477893855, Iran.

ABSTRACT
Cancer is currently a major international health problem. The development of resistance to chemotherapy has resulted in the search for herbal drugs. Ginger is a medicinal plant with several clinical applications. Metabolomics is a simultaneous detection of all the metabolites by use of (1)HNMR or mass spectroscopy and interpretation by modeling software. The purpose of this study was to detect the altered metabolites of Raji cells in the presence of ginger extract in vitro. Cells were cultured in the presence and absence of methanolic ginger extract in RPMI medium. IC50 determined by MTT and lipophilic and hydrophilic extracts were prepared from control and treated groups which were analyzed by (1)HNMR. The IC50 was 1000 μg/mL. Modeling of spectra was carried out on the two groups using OSC-PLS with MATLAB software and the main metabolites detected. Further analysis was carried out using MetaboAnalyst database. The main metabolic pathways affected by the ginger extract were detected. Ginger extract was seen to effect the protein biosynthesis, amino acid, and carbohydrate metabolism and had a strong cytotoxic effect on Raji cells in vitro.

No MeSH data available.


Related in: MedlinePlus