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The quality assessment of stored red blood cells probed using atomic-force microscopy.

Lamzin IM, Khayrullin RM - Anat Res Int (2014)

Bottom Line: The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter.Conclusion.Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC.

View Article: PubMed Central - PubMed

Affiliation: The Department of Human Anatomy, Ulyanovsk State University, Ulyanovsk 432017, Russia.

ABSTRACT
At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane's stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

No MeSH data available.


Related in: MedlinePlus

Linear regression of the change of the YM values (estimated with the usage of Hertz model) of the erythrocytes of sRBC in the course of storage.
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Related In: Results  -  Collection


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fig3: Linear regression of the change of the YM values (estimated with the usage of Hertz model) of the erythrocytes of sRBC in the course of storage.

Mentions: The result of ANOVA F-test of YM for five series of samples is F = 442,3 when P < 0,001. The change of the YM values of the erythrocytes of sRBC presents the linear regression (Figure 3). The formula of the regression was derived and defined as follows: YM = 1, 81 + 0, 04∗x, with x being the number of days RBC were stored.


The quality assessment of stored red blood cells probed using atomic-force microscopy.

Lamzin IM, Khayrullin RM - Anat Res Int (2014)

Linear regression of the change of the YM values (estimated with the usage of Hertz model) of the erythrocytes of sRBC in the course of storage.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4291138&req=5

fig3: Linear regression of the change of the YM values (estimated with the usage of Hertz model) of the erythrocytes of sRBC in the course of storage.
Mentions: The result of ANOVA F-test of YM for five series of samples is F = 442,3 when P < 0,001. The change of the YM values of the erythrocytes of sRBC presents the linear regression (Figure 3). The formula of the regression was derived and defined as follows: YM = 1, 81 + 0, 04∗x, with x being the number of days RBC were stored.

Bottom Line: The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter.Conclusion.Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC.

View Article: PubMed Central - PubMed

Affiliation: The Department of Human Anatomy, Ulyanovsk State University, Ulyanovsk 432017, Russia.

ABSTRACT
At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane's stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

No MeSH data available.


Related in: MedlinePlus