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Antioxidant enzyme inhibitor role of phosphine metal complexes in lung and leukemia cell lines.

Saygıdeğer Demir B, Keleş T, Serindağ O - Bioinorg Chem Appl (2014)

Bottom Line: C1 and C2 are water-soluble metal complexes, which may have some advantages in in vitro and in vivo studies.The effects of the above-mentioned metal complexes on thioredoxin reductase (TrxR) (EC: 1.8.1.9), glutathione peroxidase (GPx) (EC: 1.11.1.9), and catalase (Cat) (EC: 1.11.1.6) enzymes were also tested.The results of this research showed that all three metal complexes indicated dose-dependent cytotoxicity on A549 and K562 cell lines and that the complexes inhibited different percentages of the TrxR, GPx, and Cat enzymes of these tumor cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Science, and Letters Faculty, Osmaniye Korkut Ata University, Fakıuşağı, 80000 Osmaniye, Turkey.

ABSTRACT
Phosphine metal complexes have been recently evaluated in the field of cancer therapy. In this research, the cytotoxic effects of some metal phosphines {[PdCl2((CH2OH)2PCH2)2NCH3] (C1), [RuCl2(((CH2OH)2PCH2)2NCH3)2] (C2), [PtCl2((Ph2PCH2)2NCH3)(timin)2] (C3)} on K562 (human myelogenous leukemia cell line) and A549 (adenocarcinomic human alveolar basal epithelial cells) cells were investigated using the MTT test. C1 and C2 are water-soluble metal complexes, which may have some advantages in in vitro and in vivo studies. The effects of the above-mentioned metal complexes on thioredoxin reductase (TrxR) (EC: 1.8.1.9), glutathione peroxidase (GPx) (EC: 1.11.1.9), and catalase (Cat) (EC: 1.11.1.6) enzymes were also tested. The results of this research showed that all three metal complexes indicated dose-dependent cytotoxicity on A549 and K562 cell lines and that the complexes inhibited different percentages of the TrxR, GPx, and Cat enzymes of these tumor cells.

No MeSH data available.


Related in: MedlinePlus

Microscope images (20x) of K562 cells with controls (H2O and DMSO) and IC50 values of metal complexes for 48 and 72 hours. (a) Image of K562 cells at 72 h with distilled water as a control; (b) and (c) images of the cells at 72 h with IC50 values of C1 and C2 complexes, respectively; (d) image of the cells at 48 h, incubated with DMSO as a control of C3 complex; (e) image of the cells at 48 h, incubated with IC50 value of C3.
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fig5: Microscope images (20x) of K562 cells with controls (H2O and DMSO) and IC50 values of metal complexes for 48 and 72 hours. (a) Image of K562 cells at 72 h with distilled water as a control; (b) and (c) images of the cells at 72 h with IC50 values of C1 and C2 complexes, respectively; (d) image of the cells at 48 h, incubated with DMSO as a control of C3 complex; (e) image of the cells at 48 h, incubated with IC50 value of C3.

Mentions: Platin, palladium, and ruthenium complexes of phosphines were used to investigate their cytotoxic activity towards two different cell lines, A549 and K562. Cytotoxicity was evaluated by means of the MTT test after 24, 48, 72, and 120 hours of treatment with increasing concentrations of the aforementioned compounds. The IC50 values which were calculated from dose dependent curves can be seen in Figures 3 and 4. The results showed that A549 cells were resistant to death whereas K562 cells have low resistance in the presence of metal complexes. Considering the resistances of the cell lines, the 120-hour treatment period for K562 cells and the 24-hour treatment period for A549 cells were not studied for cytotoxicity of metal complexes. Among the 24 h treatment group, only C1 for K562 had cytotoxic activity, whereas the cell morphology of the others remained relatively unchanged due to its slow penetration of the cells (Figure 5). In the 24-hour treatment of the tested compound, C3 showed better cytotoxic activity for the A549 and K562 cell lines, at 0.158 mM and 0.05 mM, respectively. The other tested compounds, C1 and C2, showed different cytotoxicities on each cell (C1 on A549: 7.981 mM, 4.625 mM, and 4.575 mM for 48 h, 72 h, and 120 h, resp.; C1 on K562: 2.625 mM, 3.352 mM, and 2.396 mM for 24 h, 48 h, and 72 h, resp.; and C2 on A549: 7.436 mM and 5.302 mM for 72 h and 120 h, resp.) (Figures 3 and 4). The assignment of microscopic images proved that, after treating with C2, most of the A549 and K562 cells were still alive after 24 h; likewise, after 48 h, K562 cells treated with C2 were still alive, based on the microscopic view. So these incubation times were ignored for C1 and C2.


Antioxidant enzyme inhibitor role of phosphine metal complexes in lung and leukemia cell lines.

Saygıdeğer Demir B, Keleş T, Serindağ O - Bioinorg Chem Appl (2014)

Microscope images (20x) of K562 cells with controls (H2O and DMSO) and IC50 values of metal complexes for 48 and 72 hours. (a) Image of K562 cells at 72 h with distilled water as a control; (b) and (c) images of the cells at 72 h with IC50 values of C1 and C2 complexes, respectively; (d) image of the cells at 48 h, incubated with DMSO as a control of C3 complex; (e) image of the cells at 48 h, incubated with IC50 value of C3.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4291011&req=5

fig5: Microscope images (20x) of K562 cells with controls (H2O and DMSO) and IC50 values of metal complexes for 48 and 72 hours. (a) Image of K562 cells at 72 h with distilled water as a control; (b) and (c) images of the cells at 72 h with IC50 values of C1 and C2 complexes, respectively; (d) image of the cells at 48 h, incubated with DMSO as a control of C3 complex; (e) image of the cells at 48 h, incubated with IC50 value of C3.
Mentions: Platin, palladium, and ruthenium complexes of phosphines were used to investigate their cytotoxic activity towards two different cell lines, A549 and K562. Cytotoxicity was evaluated by means of the MTT test after 24, 48, 72, and 120 hours of treatment with increasing concentrations of the aforementioned compounds. The IC50 values which were calculated from dose dependent curves can be seen in Figures 3 and 4. The results showed that A549 cells were resistant to death whereas K562 cells have low resistance in the presence of metal complexes. Considering the resistances of the cell lines, the 120-hour treatment period for K562 cells and the 24-hour treatment period for A549 cells were not studied for cytotoxicity of metal complexes. Among the 24 h treatment group, only C1 for K562 had cytotoxic activity, whereas the cell morphology of the others remained relatively unchanged due to its slow penetration of the cells (Figure 5). In the 24-hour treatment of the tested compound, C3 showed better cytotoxic activity for the A549 and K562 cell lines, at 0.158 mM and 0.05 mM, respectively. The other tested compounds, C1 and C2, showed different cytotoxicities on each cell (C1 on A549: 7.981 mM, 4.625 mM, and 4.575 mM for 48 h, 72 h, and 120 h, resp.; C1 on K562: 2.625 mM, 3.352 mM, and 2.396 mM for 24 h, 48 h, and 72 h, resp.; and C2 on A549: 7.436 mM and 5.302 mM for 72 h and 120 h, resp.) (Figures 3 and 4). The assignment of microscopic images proved that, after treating with C2, most of the A549 and K562 cells were still alive after 24 h; likewise, after 48 h, K562 cells treated with C2 were still alive, based on the microscopic view. So these incubation times were ignored for C1 and C2.

Bottom Line: C1 and C2 are water-soluble metal complexes, which may have some advantages in in vitro and in vivo studies.The effects of the above-mentioned metal complexes on thioredoxin reductase (TrxR) (EC: 1.8.1.9), glutathione peroxidase (GPx) (EC: 1.11.1.9), and catalase (Cat) (EC: 1.11.1.6) enzymes were also tested.The results of this research showed that all three metal complexes indicated dose-dependent cytotoxicity on A549 and K562 cell lines and that the complexes inhibited different percentages of the TrxR, GPx, and Cat enzymes of these tumor cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Science, and Letters Faculty, Osmaniye Korkut Ata University, Fakıuşağı, 80000 Osmaniye, Turkey.

ABSTRACT
Phosphine metal complexes have been recently evaluated in the field of cancer therapy. In this research, the cytotoxic effects of some metal phosphines {[PdCl2((CH2OH)2PCH2)2NCH3] (C1), [RuCl2(((CH2OH)2PCH2)2NCH3)2] (C2), [PtCl2((Ph2PCH2)2NCH3)(timin)2] (C3)} on K562 (human myelogenous leukemia cell line) and A549 (adenocarcinomic human alveolar basal epithelial cells) cells were investigated using the MTT test. C1 and C2 are water-soluble metal complexes, which may have some advantages in in vitro and in vivo studies. The effects of the above-mentioned metal complexes on thioredoxin reductase (TrxR) (EC: 1.8.1.9), glutathione peroxidase (GPx) (EC: 1.11.1.9), and catalase (Cat) (EC: 1.11.1.6) enzymes were also tested. The results of this research showed that all three metal complexes indicated dose-dependent cytotoxicity on A549 and K562 cell lines and that the complexes inhibited different percentages of the TrxR, GPx, and Cat enzymes of these tumor cells.

No MeSH data available.


Related in: MedlinePlus