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Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein.

Li Y, Chen X, Xue J, Liu J, Chen X, Wulasihan M - Lipids Health Dis (2014)

Bottom Line: The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP.The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

View Article: PubMed Central - PubMed

Affiliation: The Department of Internal Medicine, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang 830000, China. xinhua_chen@zju.edu.cn.

ABSTRACT

Background: To identify the chemical structure of Coreopsis tinctoria extracts and their effect and mechanism on reducing blood lipid in hyperlipemia mice.

Methods: The flavonoids were extracted from Coreopsis tinctoria. The chemical structure was identified by HPLC. 59 mice were divided randomly into 5 groups. (group 1: normal diet control; group 2: hyperlipemia model; group 3: hyperlipemia mice treated with Coreopsis tinctoria, low dose 100 mg/kg; group 4: hyperlipemia mice treated with Coreopsis tinctoria high dose group 200 mg/kg; group 5 hyperlipemia mice treated with Fenofibrate. After 2 week of hyperlipid diet, the treatment of Coreopsis tinctoria and Fenofibrate were given for another 6 weeks with continuous hyperlipid diet. The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.

Results: Compared with normal diet group, TC, TG in hyperlipemia model group increased ( P<0.01). After treatment with Coreopsis tinctoria low dose group, high dose group, TC of the hyperlipemia mice decreased (P<0.05) without increasing AST, ALT and ALP. Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP. Expression of hepatic ADRP increased in hyperlipemia mice. Coreopsis tinctoria high dose group 200 mg/kg can inhibit ADRP as Fenofibrate does.

Conclusion: The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

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Related in: MedlinePlus

HPLC/DAD analysis of extract from Xinjiang Coreopsis tinctoria. HPLC chromatographic profile of Coreopsis tinctoria extracts. (A) Total Coreopsis tinctoria extracts from dry flower top (B) total flavonoid at 348 nm. (C) the major compound marinin at 348 nm.
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Fig1: HPLC/DAD analysis of extract from Xinjiang Coreopsis tinctoria. HPLC chromatographic profile of Coreopsis tinctoria extracts. (A) Total Coreopsis tinctoria extracts from dry flower top (B) total flavonoid at 348 nm. (C) the major compound marinin at 348 nm.

Mentions: Marein, 0.8653 mg/Ml was used as standard, using HPLC/PDA system to establish a qualitative and quantitative analysis of herbs and total flavonoids. Chromatographic conditions: Shimadzu LC 20A HPLC liquid systems, Cosmosil C18 column (4.6 × 250 mm), acetonitrile -0.1% ammonium acetate (20:80) as the mobile phase, flow rate 1.0 ml/min, injection volume 10 ul; PDA detection wavelength: 280,348 nm. The results shown in Figure 1. The HPLC conditions chosen were adequate to separate compounds as shown in chromatograms presented in Figure 1. The photodiode array detection (DAD) in tandem with electron spray ionization (ESI) mass spectrometry provided chemical composition of the fraction and they were mainly aglycones and glycosides of flavanone/chalcone flavonoid type. Compound marein appears as the major peak detected. The major 12 compounds seperated from LC/PDA/ESIMS chromatogram include 3,4’,5,6,7-Pentahydroxyflavanone-O-hexoside, Chlorogenic Acid, Flavanomarein, Flavanokanin, Quercetagitin-7-O-glucoside, 3,4’,5,6,7-Pentahydroxyflavanone, 3’,5,5’,7-Tetrahydroxyflavanone-O-hexoside, Marein, 3’,5,5’,7-Tetrahydroxy flavanone Okanin, Dicaffeoylquinic acid, Coreopsin.Figure 1


Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein.

Li Y, Chen X, Xue J, Liu J, Chen X, Wulasihan M - Lipids Health Dis (2014)

HPLC/DAD analysis of extract from Xinjiang Coreopsis tinctoria. HPLC chromatographic profile of Coreopsis tinctoria extracts. (A) Total Coreopsis tinctoria extracts from dry flower top (B) total flavonoid at 348 nm. (C) the major compound marinin at 348 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4290814&req=5

Fig1: HPLC/DAD analysis of extract from Xinjiang Coreopsis tinctoria. HPLC chromatographic profile of Coreopsis tinctoria extracts. (A) Total Coreopsis tinctoria extracts from dry flower top (B) total flavonoid at 348 nm. (C) the major compound marinin at 348 nm.
Mentions: Marein, 0.8653 mg/Ml was used as standard, using HPLC/PDA system to establish a qualitative and quantitative analysis of herbs and total flavonoids. Chromatographic conditions: Shimadzu LC 20A HPLC liquid systems, Cosmosil C18 column (4.6 × 250 mm), acetonitrile -0.1% ammonium acetate (20:80) as the mobile phase, flow rate 1.0 ml/min, injection volume 10 ul; PDA detection wavelength: 280,348 nm. The results shown in Figure 1. The HPLC conditions chosen were adequate to separate compounds as shown in chromatograms presented in Figure 1. The photodiode array detection (DAD) in tandem with electron spray ionization (ESI) mass spectrometry provided chemical composition of the fraction and they were mainly aglycones and glycosides of flavanone/chalcone flavonoid type. Compound marein appears as the major peak detected. The major 12 compounds seperated from LC/PDA/ESIMS chromatogram include 3,4’,5,6,7-Pentahydroxyflavanone-O-hexoside, Chlorogenic Acid, Flavanomarein, Flavanokanin, Quercetagitin-7-O-glucoside, 3,4’,5,6,7-Pentahydroxyflavanone, 3’,5,5’,7-Tetrahydroxyflavanone-O-hexoside, Marein, 3’,5,5’,7-Tetrahydroxy flavanone Okanin, Dicaffeoylquinic acid, Coreopsin.Figure 1

Bottom Line: The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP.The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

View Article: PubMed Central - PubMed

Affiliation: The Department of Internal Medicine, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang 830000, China. xinhua_chen@zju.edu.cn.

ABSTRACT

Background: To identify the chemical structure of Coreopsis tinctoria extracts and their effect and mechanism on reducing blood lipid in hyperlipemia mice.

Methods: The flavonoids were extracted from Coreopsis tinctoria. The chemical structure was identified by HPLC. 59 mice were divided randomly into 5 groups. (group 1: normal diet control; group 2: hyperlipemia model; group 3: hyperlipemia mice treated with Coreopsis tinctoria, low dose 100 mg/kg; group 4: hyperlipemia mice treated with Coreopsis tinctoria high dose group 200 mg/kg; group 5 hyperlipemia mice treated with Fenofibrate. After 2 week of hyperlipid diet, the treatment of Coreopsis tinctoria and Fenofibrate were given for another 6 weeks with continuous hyperlipid diet. The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.

Results: Compared with normal diet group, TC, TG in hyperlipemia model group increased ( P<0.01). After treatment with Coreopsis tinctoria low dose group, high dose group, TC of the hyperlipemia mice decreased (P<0.05) without increasing AST, ALT and ALP. Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP. Expression of hepatic ADRP increased in hyperlipemia mice. Coreopsis tinctoria high dose group 200 mg/kg can inhibit ADRP as Fenofibrate does.

Conclusion: The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

Show MeSH
Related in: MedlinePlus