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Supplementation of Superfine Powder Prepared from Chaenomeles speciosa Fruit Increases Endurance Capacity in Rats via Antioxidant and Nrf2/ARE Signaling Pathway.

Chen K, You J, Tang Y, Zhou Y, Liu P, Zou D, Zhou Q, Zhang T, Zhu J, Mi M - Evid Based Complement Alternat Med (2014)

Bottom Line: According to the in vitro experiments, SCE displayed promising antioxidant activity with powerful FARP, SC-DPPH, and SC-SAR activities.Lower MDA, higher antioxidant enzymes (SOD, CAT, and GSH-Px) activities, and upregulated Nrf2/ARE mediated antioxidant enzymes (HO-1, Trx, GCLM, and GCLC) expression were also detected in the supplemented group.This study indicates that SCE is a potent antioxidant and antifatigue agent, and SCE could be a promising raw material for the food and pharmaceutical industries.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University, Shapingba District, Chongqing 400038, China.

ABSTRACT
Chaenomeles speciosa fruit is a traditional herb medicine widely used in China. In this study, superfine powder of C. speciosa fruit (SCE), ground by supersonic nitrogen airflow at -140°C, was investigated to assess its in vitro antioxidant activity and in vivo antiphysical fatigue activity. SCE was homogenous (d < 10 μm) and rich in antioxidants like polyphenols, saponins, oleanolic acid, ursolic acid, ascorbic acid, and SOD. According to the in vitro experiments, SCE displayed promising antioxidant activity with powerful FARP, SC-DPPH, and SC-SAR activities. According to the in vivo experiments, rats supplemented with SCE had prolonged exhaustive swimming time (57%) compared to the nonsupplemented rats. Meanwhile, compared to the nonsupplemented rats, the SCE-supplemented rats had higher levels of blood glucose and liver and muscular glycogen and lower levels of LA and BUN. Lower MDA, higher antioxidant enzymes (SOD, CAT, and GSH-Px) activities, and upregulated Nrf2/ARE mediated antioxidant enzymes (HO-1, Trx, GCLM, and GCLC) expression were also detected in the supplemented group. This study indicates that SCE is a potent antioxidant and antifatigue agent, and SCE could be a promising raw material for the food and pharmaceutical industries.

No MeSH data available.


Related in: MedlinePlus

Antifatigue effect of the superfine powder prepared from C. speciosa fruit (SCE). (a) Exhaustive swimming time of rats in WFST test. Values represent mean ± SE. *P < 0.05 versus Con.: control group; SCE: SCE-supplemented group. (b) Blood lactic acid and blood urea nitrogen of rats exposed to exhaustive exercise. (c) Blood glucose, muscle glycogen, and liver glycogen of rats exposed to exhaustive exercise. (d) Oxidative-antioxidative statue of rats exposed to exhaustive exercise. Fold change was equal to that of the corresponding NEx group. Values represent mean ± SE. *P < 0.05 versus NEx group; #P < 0.05 versus Ex group. NEx: nonexercise group; NEx + SCE: nonexercise with SCE supplementation group; Ex: exercise group; Ex + SCE: exercise with SCE supplementation group.
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fig2: Antifatigue effect of the superfine powder prepared from C. speciosa fruit (SCE). (a) Exhaustive swimming time of rats in WFST test. Values represent mean ± SE. *P < 0.05 versus Con.: control group; SCE: SCE-supplemented group. (b) Blood lactic acid and blood urea nitrogen of rats exposed to exhaustive exercise. (c) Blood glucose, muscle glycogen, and liver glycogen of rats exposed to exhaustive exercise. (d) Oxidative-antioxidative statue of rats exposed to exhaustive exercise. Fold change was equal to that of the corresponding NEx group. Values represent mean ± SE. *P < 0.05 versus NEx group; #P < 0.05 versus Ex group. NEx: nonexercise group; NEx + SCE: nonexercise with SCE supplementation group; Ex: exercise group; Ex + SCE: exercise with SCE supplementation group.

Mentions: A direct measure of antifatigue effect is the increase in exercise tolerance. WFST is a classical exercise model to evaluate antifatigue; it works well for evaluating the endurance of mice/rats and is highly reproducible [15–18]. Reduced susceptibility to fatigue is correlated with longer exhaustive swimming times. In this study, SCE significantly prolonged exhaustive swimming time from approximately 7 min to 11 min (Figure 2(a)). The results indicate that SCE has antifatigue activity and could increase endurance.


Supplementation of Superfine Powder Prepared from Chaenomeles speciosa Fruit Increases Endurance Capacity in Rats via Antioxidant and Nrf2/ARE Signaling Pathway.

Chen K, You J, Tang Y, Zhou Y, Liu P, Zou D, Zhou Q, Zhang T, Zhu J, Mi M - Evid Based Complement Alternat Med (2014)

Antifatigue effect of the superfine powder prepared from C. speciosa fruit (SCE). (a) Exhaustive swimming time of rats in WFST test. Values represent mean ± SE. *P < 0.05 versus Con.: control group; SCE: SCE-supplemented group. (b) Blood lactic acid and blood urea nitrogen of rats exposed to exhaustive exercise. (c) Blood glucose, muscle glycogen, and liver glycogen of rats exposed to exhaustive exercise. (d) Oxidative-antioxidative statue of rats exposed to exhaustive exercise. Fold change was equal to that of the corresponding NEx group. Values represent mean ± SE. *P < 0.05 versus NEx group; #P < 0.05 versus Ex group. NEx: nonexercise group; NEx + SCE: nonexercise with SCE supplementation group; Ex: exercise group; Ex + SCE: exercise with SCE supplementation group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: Antifatigue effect of the superfine powder prepared from C. speciosa fruit (SCE). (a) Exhaustive swimming time of rats in WFST test. Values represent mean ± SE. *P < 0.05 versus Con.: control group; SCE: SCE-supplemented group. (b) Blood lactic acid and blood urea nitrogen of rats exposed to exhaustive exercise. (c) Blood glucose, muscle glycogen, and liver glycogen of rats exposed to exhaustive exercise. (d) Oxidative-antioxidative statue of rats exposed to exhaustive exercise. Fold change was equal to that of the corresponding NEx group. Values represent mean ± SE. *P < 0.05 versus NEx group; #P < 0.05 versus Ex group. NEx: nonexercise group; NEx + SCE: nonexercise with SCE supplementation group; Ex: exercise group; Ex + SCE: exercise with SCE supplementation group.
Mentions: A direct measure of antifatigue effect is the increase in exercise tolerance. WFST is a classical exercise model to evaluate antifatigue; it works well for evaluating the endurance of mice/rats and is highly reproducible [15–18]. Reduced susceptibility to fatigue is correlated with longer exhaustive swimming times. In this study, SCE significantly prolonged exhaustive swimming time from approximately 7 min to 11 min (Figure 2(a)). The results indicate that SCE has antifatigue activity and could increase endurance.

Bottom Line: According to the in vitro experiments, SCE displayed promising antioxidant activity with powerful FARP, SC-DPPH, and SC-SAR activities.Lower MDA, higher antioxidant enzymes (SOD, CAT, and GSH-Px) activities, and upregulated Nrf2/ARE mediated antioxidant enzymes (HO-1, Trx, GCLM, and GCLC) expression were also detected in the supplemented group.This study indicates that SCE is a potent antioxidant and antifatigue agent, and SCE could be a promising raw material for the food and pharmaceutical industries.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University, Shapingba District, Chongqing 400038, China.

ABSTRACT
Chaenomeles speciosa fruit is a traditional herb medicine widely used in China. In this study, superfine powder of C. speciosa fruit (SCE), ground by supersonic nitrogen airflow at -140°C, was investigated to assess its in vitro antioxidant activity and in vivo antiphysical fatigue activity. SCE was homogenous (d < 10 μm) and rich in antioxidants like polyphenols, saponins, oleanolic acid, ursolic acid, ascorbic acid, and SOD. According to the in vitro experiments, SCE displayed promising antioxidant activity with powerful FARP, SC-DPPH, and SC-SAR activities. According to the in vivo experiments, rats supplemented with SCE had prolonged exhaustive swimming time (57%) compared to the nonsupplemented rats. Meanwhile, compared to the nonsupplemented rats, the SCE-supplemented rats had higher levels of blood glucose and liver and muscular glycogen and lower levels of LA and BUN. Lower MDA, higher antioxidant enzymes (SOD, CAT, and GSH-Px) activities, and upregulated Nrf2/ARE mediated antioxidant enzymes (HO-1, Trx, GCLM, and GCLC) expression were also detected in the supplemented group. This study indicates that SCE is a potent antioxidant and antifatigue agent, and SCE could be a promising raw material for the food and pharmaceutical industries.

No MeSH data available.


Related in: MedlinePlus