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The effects of Wubeizi ointment on the proliferation of keloid-derived fibroblasts.

Ding JC, Tang ZM, Zhai XX, Chen XH, Li JG, Zhang CX - Cell Biochem. Biophys. (2015)

Bottom Line: The primary cells of the keloid-derived fibroblasts were cultured and the effectiveness of the WBZ ointment at different concentrations was examined by MTT colorimetric methods on keloid-derived fibroblasts.The WBZ ointment showed inhibitory effects on proliferating the keloid-derived fibroblasts (P < 0.01)in a time- and dose-dependent manner.The proportion of cells in S stage was significantly higher in each of the WBZ ointment group than in the control group (P<0.01), and the proportion of G2 + M stage cells was significantly lower than that of control group, which was statistically significant (P < 0.01).The inhibitory effects of the S and G2 + M stage increased with higher drug concentrations (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, Xuzhou Central Hospital, 199# South Jiefang Road, Xuzhou, 221009, Jiangsu, China, djicun@126.com.

ABSTRACT

Unlabelled: To evaluate the effectiveness of the Wubeizi (WBZ) ointment on keloid-derived fibroblasts. The primary cells of the keloid-derived fibroblasts were cultured and the effectiveness of the WBZ ointment at different concentrations was examined by MTT colorimetric methods on keloid-derived fibroblasts. The WBZ ointment showed inhibitory effects on proliferating the keloid-derived fibroblasts (P < 0.01)in a time- and dose-dependent manner. The proportion of cells in S stage was significantly higher in each of the WBZ ointment group than in the control group (P<0.01), and the proportion of G2 + M stage cells was significantly lower than that of control group, which was statistically significant (P < 0.01).The inhibitory effects of the S and G2 + M stage increased with higher drug concentrations (P < 0.05).

Conclusion: The WBZ ointment can inhibit the proliferation of the keloid-derived fibroblasts in a time- and dose- dependent manner.

No MeSH data available.


DNA cycle of keloid-derived fibroblasts in the control group
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Related In: Results  -  Collection


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Fig2: DNA cycle of keloid-derived fibroblasts in the control group

Mentions: Compared with the control group, there were very significant differences in the number of S and G2 + M phase cells in the medium dose group (P < 0.01), and in the number of G2 + M phase cells in the low dose group (P < 0.01). Compared with the low dose group, there were very significant differences in the number of S and G2 + M phase cells in the high dose group (P < 0.01), very significant differences in the number of S phase cells in the medium dose group (P < 0.01) and significant differences in the G2 + M phase cells in the medium dose group (P < 0.05). There was a very significant difference in the number of S phase cells between the high and the medium dose groups (P < 0.01) Table 2 and Figs. 2, 3, 4 and 5.Table 2


The effects of Wubeizi ointment on the proliferation of keloid-derived fibroblasts.

Ding JC, Tang ZM, Zhai XX, Chen XH, Li JG, Zhang CX - Cell Biochem. Biophys. (2015)

DNA cycle of keloid-derived fibroblasts in the control group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4289516&req=5

Fig2: DNA cycle of keloid-derived fibroblasts in the control group
Mentions: Compared with the control group, there were very significant differences in the number of S and G2 + M phase cells in the medium dose group (P < 0.01), and in the number of G2 + M phase cells in the low dose group (P < 0.01). Compared with the low dose group, there were very significant differences in the number of S and G2 + M phase cells in the high dose group (P < 0.01), very significant differences in the number of S phase cells in the medium dose group (P < 0.01) and significant differences in the G2 + M phase cells in the medium dose group (P < 0.05). There was a very significant difference in the number of S phase cells between the high and the medium dose groups (P < 0.01) Table 2 and Figs. 2, 3, 4 and 5.Table 2

Bottom Line: The primary cells of the keloid-derived fibroblasts were cultured and the effectiveness of the WBZ ointment at different concentrations was examined by MTT colorimetric methods on keloid-derived fibroblasts.The WBZ ointment showed inhibitory effects on proliferating the keloid-derived fibroblasts (P < 0.01)in a time- and dose-dependent manner.The proportion of cells in S stage was significantly higher in each of the WBZ ointment group than in the control group (P<0.01), and the proportion of G2 + M stage cells was significantly lower than that of control group, which was statistically significant (P < 0.01).The inhibitory effects of the S and G2 + M stage increased with higher drug concentrations (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, Xuzhou Central Hospital, 199# South Jiefang Road, Xuzhou, 221009, Jiangsu, China, djicun@126.com.

ABSTRACT

Unlabelled: To evaluate the effectiveness of the Wubeizi (WBZ) ointment on keloid-derived fibroblasts. The primary cells of the keloid-derived fibroblasts were cultured and the effectiveness of the WBZ ointment at different concentrations was examined by MTT colorimetric methods on keloid-derived fibroblasts. The WBZ ointment showed inhibitory effects on proliferating the keloid-derived fibroblasts (P < 0.01)in a time- and dose-dependent manner. The proportion of cells in S stage was significantly higher in each of the WBZ ointment group than in the control group (P<0.01), and the proportion of G2 + M stage cells was significantly lower than that of control group, which was statistically significant (P < 0.01).The inhibitory effects of the S and G2 + M stage increased with higher drug concentrations (P < 0.05).

Conclusion: The WBZ ointment can inhibit the proliferation of the keloid-derived fibroblasts in a time- and dose- dependent manner.

No MeSH data available.