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Mosquito Larvicidal Potential of Gossypium hirsutum (Bt cotton) Leaves Extracts against Aedes aegypti and Anopheles stephensi larvae.

Patil CD, Borase HP, Salunkhe RB, Suryawanshi RK, Narkhade CP, Salunke BK, Patil SV - J Arthropod Borne Dis (2013)

Bottom Line: Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2(nd) and 3(rd) instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2(nd) and 3(rd) instars of An. stephensi, respectively.Crude protein from the water extract was precipitated using acetone and tested against 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi.It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi, respectively.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, North Maharashtra University, Maharashtra, India.

ABSTRACT

Background: We aimed to extract the ingredients from leaves of Gossypium hirsutum (Bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, Aedes aegypti and Anopheles stephensi.

Methods: Qualitative and quantitative estimation of ingredients from Go. hirsutum (Bt) plant extract was carried out and their inhibitory action against mosquito larvae was determined using mosquito larvicidal assay.

Results: LC50 values of water, ethanol, ethyl acetate and hexane extracts for Ae. aegypti were 211.73±21.49, 241.64±19.92, 358.07±32.43, 401.03±36.19 and 232.56±26.00, 298.54±21.78, 366.50±30.59, 387.19±31.82 for 4(th) instar of An. stephensi, respectively. The water extract displayed lowest LC50 value followed by ethanol, ethyl acetate and hexane. Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2(nd) and 3(rd) instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2(nd) and 3(rd) instars of An. stephensi, respectively. Crude protein from the water extract was precipitated using acetone and tested against 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi. It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi, respectively.

Conclusion: Leaves extracts of Go. hirsutum (Bt) is potential mosquito larvicide and can be used as a potent alternative to chemical insecticides in integrated pest management.

No MeSH data available.


Related in: MedlinePlus

A) Bt cotton (Gossypium hirsutum) plant with flower and fruit, (B) Bt cotton plantation in field.
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f1-jad-8-91: A) Bt cotton (Gossypium hirsutum) plant with flower and fruit, (B) Bt cotton plantation in field.

Mentions: Gossypium hirsutum (Malvaceae: Malvales) is commonly known as upland cotton or Mexican cotton (Fig. 1). It is native from Central America and Mexico and an important fibre crop and most widely cultivated species. Worldwide around 35 mha land is under cotton crop. India alone constitutes 9.5 mha about one quarter of global area under this crop. The country reported a little over 21% of the global cotton production in 2008–2009 and stands second in cotton production having 4.9 million tons behind China having 7.8 million tons (Karihaloo and Kumar 2009). However, this production is less as compared to land under cultivation. Low production is majorly attributed to attack by sap sucking pest Helicoverpa armigera commonly known as bollworm. Advancement in plant biotechnology with introduction of genetically modified (GM) variety of cotton known as ‘Bt- cotton’ by Mahyco seeds in collaboration with Monsanto has improved situation of He. armigera attack and production of cotton in India. The Bt cotton variety involves incorporation of Cry1Ac gene from common soil bacterium Bacillus thuringiensis, which gives protection against bollworm. Apart from prime source of fibre used in textiles cotton crop also reported to have some medicinal uses. Leaves of cotton plant show antibacterial activity against clinically important bacteria like Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella dysenteriae (Omojasola and Awe 2004). Leaves of cotton in combination with other plant extracts are effective against malaria (Igwe et al. 2012) and good remedy for uterine fibroid and cancer (Hartwel 1971) and as antifertility agent (Randel et al. 1992).


Mosquito Larvicidal Potential of Gossypium hirsutum (Bt cotton) Leaves Extracts against Aedes aegypti and Anopheles stephensi larvae.

Patil CD, Borase HP, Salunkhe RB, Suryawanshi RK, Narkhade CP, Salunke BK, Patil SV - J Arthropod Borne Dis (2013)

A) Bt cotton (Gossypium hirsutum) plant with flower and fruit, (B) Bt cotton plantation in field.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4289515&req=5

f1-jad-8-91: A) Bt cotton (Gossypium hirsutum) plant with flower and fruit, (B) Bt cotton plantation in field.
Mentions: Gossypium hirsutum (Malvaceae: Malvales) is commonly known as upland cotton or Mexican cotton (Fig. 1). It is native from Central America and Mexico and an important fibre crop and most widely cultivated species. Worldwide around 35 mha land is under cotton crop. India alone constitutes 9.5 mha about one quarter of global area under this crop. The country reported a little over 21% of the global cotton production in 2008–2009 and stands second in cotton production having 4.9 million tons behind China having 7.8 million tons (Karihaloo and Kumar 2009). However, this production is less as compared to land under cultivation. Low production is majorly attributed to attack by sap sucking pest Helicoverpa armigera commonly known as bollworm. Advancement in plant biotechnology with introduction of genetically modified (GM) variety of cotton known as ‘Bt- cotton’ by Mahyco seeds in collaboration with Monsanto has improved situation of He. armigera attack and production of cotton in India. The Bt cotton variety involves incorporation of Cry1Ac gene from common soil bacterium Bacillus thuringiensis, which gives protection against bollworm. Apart from prime source of fibre used in textiles cotton crop also reported to have some medicinal uses. Leaves of cotton plant show antibacterial activity against clinically important bacteria like Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella dysenteriae (Omojasola and Awe 2004). Leaves of cotton in combination with other plant extracts are effective against malaria (Igwe et al. 2012) and good remedy for uterine fibroid and cancer (Hartwel 1971) and as antifertility agent (Randel et al. 1992).

Bottom Line: Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2(nd) and 3(rd) instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2(nd) and 3(rd) instars of An. stephensi, respectively.Crude protein from the water extract was precipitated using acetone and tested against 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi.It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi, respectively.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, North Maharashtra University, Maharashtra, India.

ABSTRACT

Background: We aimed to extract the ingredients from leaves of Gossypium hirsutum (Bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, Aedes aegypti and Anopheles stephensi.

Methods: Qualitative and quantitative estimation of ingredients from Go. hirsutum (Bt) plant extract was carried out and their inhibitory action against mosquito larvae was determined using mosquito larvicidal assay.

Results: LC50 values of water, ethanol, ethyl acetate and hexane extracts for Ae. aegypti were 211.73±21.49, 241.64±19.92, 358.07±32.43, 401.03±36.19 and 232.56±26.00, 298.54±21.78, 366.50±30.59, 387.19±31.82 for 4(th) instar of An. stephensi, respectively. The water extract displayed lowest LC50 value followed by ethanol, ethyl acetate and hexane. Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2(nd) and 3(rd) instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2(nd) and 3(rd) instars of An. stephensi, respectively. Crude protein from the water extract was precipitated using acetone and tested against 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi. It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2(nd), 3(rd) and 4(th) instars of Ae. aegypti and An. stephensi, respectively.

Conclusion: Leaves extracts of Go. hirsutum (Bt) is potential mosquito larvicide and can be used as a potent alternative to chemical insecticides in integrated pest management.

No MeSH data available.


Related in: MedlinePlus