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Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Wannigama DL, Dwivedi R, Zahraei-Ramazani A - J Arthropod Borne Dis (2013)

Bottom Line: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments.However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin.For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Centre of Advanced Study, Banaras Hindu University, Varanasi, India.

ABSTRACT

Background: Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Methods: Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.

Results: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04%) and Bl. germanica (35.96%). However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

Conclusion: Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.

No MeSH data available.


Related in: MedlinePlus

Distribution (%) of the cockroaches infestation at food-handling establishments
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f2-jad-8-10: Distribution (%) of the cockroaches infestation at food-handling establishments

Mentions: A total 52 food- handling establishments studied, 40(77%) had cockroaches infestation (Fig. 2): 21(53%) with Pe. americana, 8(20%) with Bl. germanica, and 11(28%) with both species. Totally 124 adult cockroaches were collected from food-handling establishments: 52 Pe. americana, 46 with Bl. germanica. In the higher rate of Pe. americana were collected from bakeries (36.3%) and grocery stores (42.5%). The collection rate of Pe. americana obtained in food storage stores, restaurants and small food processing plants were 12.7, 2.3 and 6.2%, respectively. Bl. germanica was highly founded in the food storage stores (28.3%), small food processing plants (18.1%), restaurants (7.6%), also bakeries (16.3%) and grocery stores (29.7%).


Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Wannigama DL, Dwivedi R, Zahraei-Ramazani A - J Arthropod Borne Dis (2013)

Distribution (%) of the cockroaches infestation at food-handling establishments
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4289503&req=5

f2-jad-8-10: Distribution (%) of the cockroaches infestation at food-handling establishments
Mentions: A total 52 food- handling establishments studied, 40(77%) had cockroaches infestation (Fig. 2): 21(53%) with Pe. americana, 8(20%) with Bl. germanica, and 11(28%) with both species. Totally 124 adult cockroaches were collected from food-handling establishments: 52 Pe. americana, 46 with Bl. germanica. In the higher rate of Pe. americana were collected from bakeries (36.3%) and grocery stores (42.5%). The collection rate of Pe. americana obtained in food storage stores, restaurants and small food processing plants were 12.7, 2.3 and 6.2%, respectively. Bl. germanica was highly founded in the food storage stores (28.3%), small food processing plants (18.1%), restaurants (7.6%), also bakeries (16.3%) and grocery stores (29.7%).

Bottom Line: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments.However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin.For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Centre of Advanced Study, Banaras Hindu University, Varanasi, India.

ABSTRACT

Background: Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Methods: Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.

Results: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04%) and Bl. germanica (35.96%). However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

Conclusion: Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.

No MeSH data available.


Related in: MedlinePlus