Limits...
Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Wannigama DL, Dwivedi R, Zahraei-Ramazani A - J Arthropod Borne Dis (2013)

Bottom Line: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments.However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin.For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Centre of Advanced Study, Banaras Hindu University, Varanasi, India.

ABSTRACT

Background: Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Methods: Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.

Results: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04%) and Bl. germanica (35.96%). However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

Conclusion: Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.

No MeSH data available.


Related in: MedlinePlus

Distribution (%) of the cockroaches infestation at households
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4289503&req=5

f1-jad-8-10: Distribution (%) of the cockroaches infestation at households

Mentions: Among the 44 households studied, 28(54%) had cockroaches infestation: 16(57%) with Pe. americana, 5(18%) with Bl. germanica, and 7 (25%) with both species. Totally 79 adult cockroaches were collected from households (Fig. 1): 52 Pe. americana, 27 with Bl. germanica. In households, most of the Pe. americana were collected from (66.7%) kitchen, also 16.4% in bed room, 9.8% in dining room and less than 3% in toilet and living room. As well as the higher rate of Bl. germanica was found in the storage room (41.3%), sleeping room (25.1%) and kitchen (22.6%). The occurrence of Bl. germanica in dining room and living room was 4.3 and 6.7%, respectively.


Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Wannigama DL, Dwivedi R, Zahraei-Ramazani A - J Arthropod Borne Dis (2013)

Distribution (%) of the cockroaches infestation at households
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4289503&req=5

f1-jad-8-10: Distribution (%) of the cockroaches infestation at households
Mentions: Among the 44 households studied, 28(54%) had cockroaches infestation: 16(57%) with Pe. americana, 5(18%) with Bl. germanica, and 7 (25%) with both species. Totally 79 adult cockroaches were collected from households (Fig. 1): 52 Pe. americana, 27 with Bl. germanica. In households, most of the Pe. americana were collected from (66.7%) kitchen, also 16.4% in bed room, 9.8% in dining room and less than 3% in toilet and living room. As well as the higher rate of Bl. germanica was found in the storage room (41.3%), sleeping room (25.1%) and kitchen (22.6%). The occurrence of Bl. germanica in dining room and living room was 4.3 and 6.7%, respectively.

Bottom Line: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments.However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin.For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, Centre of Advanced Study, Banaras Hindu University, Varanasi, India.

ABSTRACT

Background: Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

Methods: Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.

Results: Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04%) and Bl. germanica (35.96%). However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.

Conclusion: Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.

No MeSH data available.


Related in: MedlinePlus