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Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

Zhou ZY, Li F, Cheng SP, Huang H, Peng BW, Wang J, Liu CM, Xing C, Sun YL, Bsoul N, Pan H, Yi CJ, Liu RH, Zhong GJ - Med. Sci. Monit. (2015)

Bottom Line: The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively.After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01).Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, China (mainland).

ABSTRACT

Background: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.

Material/methods: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.

Results: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01).

Conclusions: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats.

Show MeSH
Testosterone concentrations in rat serum. Testosterone concentrations in rat serum at 12 weeks after IGFBP-3 shRNA treatment were determined by ELISA. The data were collected from 9 rats in each group. * p<0.01, compared to diabetic control.
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f3-medscimonit-21-94: Testosterone concentrations in rat serum. Testosterone concentrations in rat serum at 12 weeks after IGFBP-3 shRNA treatment were determined by ELISA. The data were collected from 9 rats in each group. * p<0.01, compared to diabetic control.

Mentions: Real-time qPCR analysis using GAPDH as a housekeeping gene showed that cavernous IGFBP-3 mRNA level in the IGFBP-3 shRNA treatment group was significantly lower than in the diabetic control group (P<0.01, Figure 2). Accordingly, Western blot analysis showed that cavernous IGFBP-3 protein level in the IGFBP-3 shRNA treatment group was significantly lower than in the diabetic control group (P<0.01, Figure 3, lower). These results are consistent with the results of real-time qPCR analysis and prove that IGFBP-3 shRNA inhibits IGFBP-3 expression in rat cavernous tissue.


Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

Zhou ZY, Li F, Cheng SP, Huang H, Peng BW, Wang J, Liu CM, Xing C, Sun YL, Bsoul N, Pan H, Yi CJ, Liu RH, Zhong GJ - Med. Sci. Monit. (2015)

Testosterone concentrations in rat serum. Testosterone concentrations in rat serum at 12 weeks after IGFBP-3 shRNA treatment were determined by ELISA. The data were collected from 9 rats in each group. * p<0.01, compared to diabetic control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4289482&req=5

f3-medscimonit-21-94: Testosterone concentrations in rat serum. Testosterone concentrations in rat serum at 12 weeks after IGFBP-3 shRNA treatment were determined by ELISA. The data were collected from 9 rats in each group. * p<0.01, compared to diabetic control.
Mentions: Real-time qPCR analysis using GAPDH as a housekeeping gene showed that cavernous IGFBP-3 mRNA level in the IGFBP-3 shRNA treatment group was significantly lower than in the diabetic control group (P<0.01, Figure 2). Accordingly, Western blot analysis showed that cavernous IGFBP-3 protein level in the IGFBP-3 shRNA treatment group was significantly lower than in the diabetic control group (P<0.01, Figure 3, lower). These results are consistent with the results of real-time qPCR analysis and prove that IGFBP-3 shRNA inhibits IGFBP-3 expression in rat cavernous tissue.

Bottom Line: The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively.After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01).Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, China (mainland).

ABSTRACT

Background: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.

Material/methods: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.

Results: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01).

Conclusions: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats.

Show MeSH