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Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

Zhou ZY, Li F, Cheng SP, Huang H, Peng BW, Wang J, Liu CM, Xing C, Sun YL, Bsoul N, Pan H, Yi CJ, Liu RH, Zhong GJ - Med. Sci. Monit. (2015)

Bottom Line: The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively.After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01).Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, China (mainland).

ABSTRACT

Background: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.

Material/methods: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.

Results: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01).

Conclusions: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats.

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Evaluation of erectile function. Representative ICP responses to cavernous nerves electrical stimulation at 12 weeks after IGFBP-3 shRNA treatment in the normal control group (A), diabetic control group (B) (n=9), and IGFBP-3 shRNA treatment group (C). The bold line indicates 1 min of electrical stimulation to the cavernous nerves. The data on ICP/MAP (D) and total ICP (the area under curve) (E) were collected from 9 rats in each group. * p<0.01, compared to diabetic control. MAP: mean arterial pressure; ICP: intracavernous pressure.
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f1-medscimonit-21-94: Evaluation of erectile function. Representative ICP responses to cavernous nerves electrical stimulation at 12 weeks after IGFBP-3 shRNA treatment in the normal control group (A), diabetic control group (B) (n=9), and IGFBP-3 shRNA treatment group (C). The bold line indicates 1 min of electrical stimulation to the cavernous nerves. The data on ICP/MAP (D) and total ICP (the area under curve) (E) were collected from 9 rats in each group. * p<0.01, compared to diabetic control. MAP: mean arterial pressure; ICP: intracavernous pressure.

Mentions: We determined the ratio of ICP/MAP and the total ICP (area under curve) during electrostimulation of the cavernous nerves at 12 weeks after IGFBP-3 shRNA administration. The representative ICP tracing in response to electrostimulation of the cavernous nerves is shown in Figure 1(A–C). Electrostimulation elicited significantly increased ICP/MAP ratio (Figure 1D) and total ICP (Figure 1E) in the IGFBP-3 shRNA treatment group compared to those in the diabetic control group (p<0.01, respectively). These data suggest that IGFBP-3 shRNA administration rehabilitated erectile function in diabetic rats.


Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

Zhou ZY, Li F, Cheng SP, Huang H, Peng BW, Wang J, Liu CM, Xing C, Sun YL, Bsoul N, Pan H, Yi CJ, Liu RH, Zhong GJ - Med. Sci. Monit. (2015)

Evaluation of erectile function. Representative ICP responses to cavernous nerves electrical stimulation at 12 weeks after IGFBP-3 shRNA treatment in the normal control group (A), diabetic control group (B) (n=9), and IGFBP-3 shRNA treatment group (C). The bold line indicates 1 min of electrical stimulation to the cavernous nerves. The data on ICP/MAP (D) and total ICP (the area under curve) (E) were collected from 9 rats in each group. * p<0.01, compared to diabetic control. MAP: mean arterial pressure; ICP: intracavernous pressure.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4289482&req=5

f1-medscimonit-21-94: Evaluation of erectile function. Representative ICP responses to cavernous nerves electrical stimulation at 12 weeks after IGFBP-3 shRNA treatment in the normal control group (A), diabetic control group (B) (n=9), and IGFBP-3 shRNA treatment group (C). The bold line indicates 1 min of electrical stimulation to the cavernous nerves. The data on ICP/MAP (D) and total ICP (the area under curve) (E) were collected from 9 rats in each group. * p<0.01, compared to diabetic control. MAP: mean arterial pressure; ICP: intracavernous pressure.
Mentions: We determined the ratio of ICP/MAP and the total ICP (area under curve) during electrostimulation of the cavernous nerves at 12 weeks after IGFBP-3 shRNA administration. The representative ICP tracing in response to electrostimulation of the cavernous nerves is shown in Figure 1(A–C). Electrostimulation elicited significantly increased ICP/MAP ratio (Figure 1D) and total ICP (Figure 1E) in the IGFBP-3 shRNA treatment group compared to those in the diabetic control group (p<0.01, respectively). These data suggest that IGFBP-3 shRNA administration rehabilitated erectile function in diabetic rats.

Bottom Line: The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively.After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01).Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, China (mainland).

ABSTRACT

Background: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.

Material/methods: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.

Results: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01).

Conclusions: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats.

Show MeSH