Limits...
Clostridium tertium bacteremia in a patient with glyphosate ingestion.

You MJ, Shin GW, Lee CS - Am J Case Rep (2015)

Bottom Line: The 16S rRNA sequences from all colonies were 99% identical with that of C. tertium (AB618789) found on a BLAST search of the NCBI database.However, it was resistant to third- and fourth-generation cephalosporins.The results highlight the need for careful diagnosis and selection of antibiotics in the treatment of this organism.

View Article: PubMed Central - PubMed

Affiliation: Bio-safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea.

ABSTRACT

Background: Clostridium tertium is distributed in the soil and in animal and human gastrointestinal tracts. C. tertium has been isolated from patients with blood diseases, immune disorders, and abdominal surgeries. Glyphosate is toxic, causing cause eye and skin irritation, gastrointestinal pain, and vomiting. Ingestion of herbicides modifies the gastrointestinal environment, which stresses the living organisms. However, there has been little attention to cases of bacteremia in patients recovering from suicide attempt by ingesting herbicide.

Case report: Clostridium tertium was identified in a 44-year-old female who attempted suicide by glyphosate (a herbicide) ingestion. The 16S rRNA sequences from all colonies were 99% identical with that of C. tertium (AB618789) found on a BLAST search of the NCBI database. The bacterium was cultured on TSA under aerobic and anaerobic conditions. Antimicrobial susceptibility tests performed under both aerobic and anaerobic conditions showed that the bacterium was susceptible to penicillin, a combination of β-lactamase inhibitor and piperacillin or amoxicillin, and first- and second- generation cephalosporins. However, it was resistant to third- and fourth-generation cephalosporins.

Conclusions: Glyphosate herbicide might be a predisposing factor responsible for the pathogenesis of C. tertium. The results highlight the need for careful diagnosis and selection of antibiotics in the treatment of this organism.

Show MeSH

Related in: MedlinePlus

Spore stain of Clostridium tertium on two different cultivations, aerobic (A) and anaerobic (B) conditions.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4289481&req=5

f1-amjcaserep-16-4: Spore stain of Clostridium tertium on two different cultivations, aerobic (A) and anaerobic (B) conditions.

Mentions: Under aerobic condition, morphology and staining of the bacterium were similar with that from initial blood culture (Figure 1A). Under anaerobic conditions, the bacterium showed a tennis racquet-like shape with terminally located ova with blue color, indicating spore formation (Figure 1B). Antibiotic susceptibility tests for the bacterium were also performed under aerobic and anaerobic conditions. Although there were differences in susceptibilities to most antibiotics between both conditions, there is no difference in susceptibilities of C. tertium strain to antibiotics on interpretation based on clear zone diameter. Under both aerobic and anaerobic conditions, C. tertium was sensitive to penicillin, piperacillin/tazobactam, Amoxicillin/clavulanic acid, Cephalothin, Cefoxitin, Imipenem, Moxifloxacin, Vancomycin, Tetracycline, Rifampicin, and Sulfamethoxazole/Trimethoprim but resistance to Ceftiofur, Cefotaxime, Ceftazidime, Cefepime, Amikacin, Gentamicin, Clindamycin, and Metronidazole (Table 1).


Clostridium tertium bacteremia in a patient with glyphosate ingestion.

You MJ, Shin GW, Lee CS - Am J Case Rep (2015)

Spore stain of Clostridium tertium on two different cultivations, aerobic (A) and anaerobic (B) conditions.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4289481&req=5

f1-amjcaserep-16-4: Spore stain of Clostridium tertium on two different cultivations, aerobic (A) and anaerobic (B) conditions.
Mentions: Under aerobic condition, morphology and staining of the bacterium were similar with that from initial blood culture (Figure 1A). Under anaerobic conditions, the bacterium showed a tennis racquet-like shape with terminally located ova with blue color, indicating spore formation (Figure 1B). Antibiotic susceptibility tests for the bacterium were also performed under aerobic and anaerobic conditions. Although there were differences in susceptibilities to most antibiotics between both conditions, there is no difference in susceptibilities of C. tertium strain to antibiotics on interpretation based on clear zone diameter. Under both aerobic and anaerobic conditions, C. tertium was sensitive to penicillin, piperacillin/tazobactam, Amoxicillin/clavulanic acid, Cephalothin, Cefoxitin, Imipenem, Moxifloxacin, Vancomycin, Tetracycline, Rifampicin, and Sulfamethoxazole/Trimethoprim but resistance to Ceftiofur, Cefotaxime, Ceftazidime, Cefepime, Amikacin, Gentamicin, Clindamycin, and Metronidazole (Table 1).

Bottom Line: The 16S rRNA sequences from all colonies were 99% identical with that of C. tertium (AB618789) found on a BLAST search of the NCBI database.However, it was resistant to third- and fourth-generation cephalosporins.The results highlight the need for careful diagnosis and selection of antibiotics in the treatment of this organism.

View Article: PubMed Central - PubMed

Affiliation: Bio-safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea.

ABSTRACT

Background: Clostridium tertium is distributed in the soil and in animal and human gastrointestinal tracts. C. tertium has been isolated from patients with blood diseases, immune disorders, and abdominal surgeries. Glyphosate is toxic, causing cause eye and skin irritation, gastrointestinal pain, and vomiting. Ingestion of herbicides modifies the gastrointestinal environment, which stresses the living organisms. However, there has been little attention to cases of bacteremia in patients recovering from suicide attempt by ingesting herbicide.

Case report: Clostridium tertium was identified in a 44-year-old female who attempted suicide by glyphosate (a herbicide) ingestion. The 16S rRNA sequences from all colonies were 99% identical with that of C. tertium (AB618789) found on a BLAST search of the NCBI database. The bacterium was cultured on TSA under aerobic and anaerobic conditions. Antimicrobial susceptibility tests performed under both aerobic and anaerobic conditions showed that the bacterium was susceptible to penicillin, a combination of β-lactamase inhibitor and piperacillin or amoxicillin, and first- and second- generation cephalosporins. However, it was resistant to third- and fourth-generation cephalosporins.

Conclusions: Glyphosate herbicide might be a predisposing factor responsible for the pathogenesis of C. tertium. The results highlight the need for careful diagnosis and selection of antibiotics in the treatment of this organism.

Show MeSH
Related in: MedlinePlus