Limits...
RAB8A a new biomarker for endometrial cancer?

Bie Y, Zhang Z - World J Surg Oncol (2014)

Bottom Line: A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS).Differential proteins were identified by Western blot analysis.The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China. bjzhangzhy@sina.cn.

ABSTRACT

Background: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).

Methods: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.

Results: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.

Conclusions: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.

Show MeSH

Related in: MedlinePlus

The comparison of protein function between upregulated and downregulated proteins. Functions of transporter activity, catalytic activity and antioxidant activity are significantly higher in upregulated proteins (P <0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4289371&req=5

Fig2: The comparison of protein function between upregulated and downregulated proteins. Functions of transporter activity, catalytic activity and antioxidant activity are significantly higher in upregulated proteins (P <0.05).

Mentions: Protein expression of cells, including DCs and DCs with HEC-1-B cell lysate pulsed (HEC-DCs), were analyzed by LC-MS/MS. We found 136 upregulated proteins and 110 downregulated proteins in HEC-DCs compared with DCs. Figure 2 shows the comparison of protein function between upregulated and downregulated proteins. Some proteins that were upregulated were chosen to be analyzed by Western blotting. We found that the expression of Ras-related protein Rab-8A (RAB8A) is significantly higher in HEC-DC than in DCs (Figure 3). We also compared its expression in tissues of healthy and malignant endometrium. A significant increase of RAB8A in EC versus healthy controls was observed (P <0.05) (Figure 4).Figure 2


RAB8A a new biomarker for endometrial cancer?

Bie Y, Zhang Z - World J Surg Oncol (2014)

The comparison of protein function between upregulated and downregulated proteins. Functions of transporter activity, catalytic activity and antioxidant activity are significantly higher in upregulated proteins (P <0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289371&req=5

Fig2: The comparison of protein function between upregulated and downregulated proteins. Functions of transporter activity, catalytic activity and antioxidant activity are significantly higher in upregulated proteins (P <0.05).
Mentions: Protein expression of cells, including DCs and DCs with HEC-1-B cell lysate pulsed (HEC-DCs), were analyzed by LC-MS/MS. We found 136 upregulated proteins and 110 downregulated proteins in HEC-DCs compared with DCs. Figure 2 shows the comparison of protein function between upregulated and downregulated proteins. Some proteins that were upregulated were chosen to be analyzed by Western blotting. We found that the expression of Ras-related protein Rab-8A (RAB8A) is significantly higher in HEC-DC than in DCs (Figure 3). We also compared its expression in tissues of healthy and malignant endometrium. A significant increase of RAB8A in EC versus healthy controls was observed (P <0.05) (Figure 4).Figure 2

Bottom Line: A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS).Differential proteins were identified by Western blot analysis.The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China. bjzhangzhy@sina.cn.

ABSTRACT

Background: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).

Methods: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.

Results: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.

Conclusions: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.

Show MeSH
Related in: MedlinePlus