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RAB8A a new biomarker for endometrial cancer?

Bie Y, Zhang Z - World J Surg Oncol (2014)

Bottom Line: A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS).Differential proteins were identified by Western blot analysis.The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China. bjzhangzhy@sina.cn.

ABSTRACT

Background: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).

Methods: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.

Results: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.

Conclusions: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.

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Related in: MedlinePlus

Flow cytometric analysis of the seventh day DCs. A-C show the cells were cultured by the adherent method, and D-F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+(B, E) and CD11C+/CD80+(C, F). DCs dendritic cells.
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Fig1: Flow cytometric analysis of the seventh day DCs. A-C show the cells were cultured by the adherent method, and D-F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+(B, E) and CD11C+/CD80+(C, F). DCs dendritic cells.

Mentions: Figure 1 is flow cytometric analysis result. Figure 1A to C show that the DCs were cultured using UBMC by the adherent method and Figure 1D to F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+ (Figure 1B and E) and CD11C+/CD80+ (Figure 1C and F). From the flow cytometric analysis we can conclude that the tumor cell lysate of EC can mature DCs.Figure 1


RAB8A a new biomarker for endometrial cancer?

Bie Y, Zhang Z - World J Surg Oncol (2014)

Flow cytometric analysis of the seventh day DCs. A-C show the cells were cultured by the adherent method, and D-F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+(B, E) and CD11C+/CD80+(C, F). DCs dendritic cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289371&req=5

Fig1: Flow cytometric analysis of the seventh day DCs. A-C show the cells were cultured by the adherent method, and D-F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+(B, E) and CD11C+/CD80+(C, F). DCs dendritic cells.
Mentions: Figure 1 is flow cytometric analysis result. Figure 1A to C show that the DCs were cultured using UBMC by the adherent method and Figure 1D to F show that the DCs were pulsed with HEC-1-B cell lysate. The cells were harvested at the seventh day of culture. The cells in the gated population were further analyzed and found to be CD11C+/HLA-DR+ (Figure 1B and E) and CD11C+/CD80+ (Figure 1C and F). From the flow cytometric analysis we can conclude that the tumor cell lysate of EC can mature DCs.Figure 1

Bottom Line: A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS).Differential proteins were identified by Western blot analysis.The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China. bjzhangzhy@sina.cn.

ABSTRACT

Background: We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).

Methods: Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.

Results: The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.

Conclusions: RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.

Show MeSH
Related in: MedlinePlus