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The inhibitory effect of colloidal bismuth hydroxide gel on Escherichia coli O157:H7 and on the activity of Shiga toxins.

Subils T, Casabonne C, Balagué C - BMC Res Notes (2014)

Bottom Line: Effect on EDL933 virulence factors: significant decrease in active Stx and 933 W Stx phage titer.ELISA did not find significant differences with treatment.The results obtained may be useful in the development of new therapeutic strategies based on the use of CBHG to prevent or improve the prognosis of HUS, as it can be used to control STEC infections.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biochemistry and Pharmaceutical Sciences, Department of Clinical Bacteriology, National University of Rosario, Suipacha 531, S2002LRK Rosario, Santa Fe, Argentina. tomassubils@hotmail.com.

ABSTRACT

Background: Shiga toxin-producing Escherichia coli (STEC) is the causative agent of hemolytic uremic syndrome (HUS). Colloidal bismuth hydroxide gel (CBHG) is an anti-diarrheal and antisecretory compound, which does not inhibit gastrointestinal motility and reaches an in vivo gut concentration of 10.8 mg/ml of bismuth. Its action on bacteria has not been studied. We analyzed its inhibitory effects on STEC, as well as the deactivation of the Shiga toxin (Stx) and its ability to block the spread of genes encoding Stx. We determined a minimum inhibitory concentration and bactericidal concentration for the STEC O157:H7 strain (EDL933), with CBHG and Chobet® bismuth cream with pectin (CBCHP). We analyzed its effect on Stx by means of cytotoxicity assay and ELISA, as well as its effect on the free 933 W Stx phage.

Results: Effect on the EDL933 strain: CBHG: MIC 10 mg/ml of bismuth.

Cbchp: MIC 6 mg/ml and MBC 15 mg/ml of bismuth. Effect on EDL933 virulence factors: significant decrease in active Stx and 933 W Stx phage titer. ELISA did not find significant differences with treatment.

Conclusions: The results obtained may be useful in the development of new therapeutic strategies based on the use of CBHG to prevent or improve the prognosis of HUS, as it can be used to control STEC infections.

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Related in: MedlinePlus

Photographs of incubated phage lysis plates. A. Without bismuth. B. With 1.25 mg/ml of bismuth. C. With 5 mg/ml of bismuth. D. With 10 mg/ml of bismuth.
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Fig2: Photographs of incubated phage lysis plates. A. Without bismuth. B. With 1.25 mg/ml of bismuth. C. With 5 mg/ml of bismuth. D. With 10 mg/ml of bismuth.

Mentions: As for the effect of the compounds on the Stx 933 W-encoding phage, a statistically significant decrease (p < 0.05) was observed in the titer of the phage treated with both CBHG and CBCHP in comparison with the untreated titer. The decrease in the titer of the phage was 90% for a CBHG dilution of 1/3, which corresponds to a bismuth concentration of 10 mg/ml; and 86% for a CBHG dilution of 1/6, which is equivalent to a bismuth concentration of 5 mg/ml. In the tests performed with CBCHP, the decrease in the titer of the phage was 82% for a CBCHP dilution of 1/3, which corresponds to a bismuth concentration of 10 mg/ml; and 73% for a CBCHP dilution of 1/6, which is equivalent to a bismuth concentration of 5 mg/ml (Table 3 and Figure 2).Table 3


The inhibitory effect of colloidal bismuth hydroxide gel on Escherichia coli O157:H7 and on the activity of Shiga toxins.

Subils T, Casabonne C, Balagué C - BMC Res Notes (2014)

Photographs of incubated phage lysis plates. A. Without bismuth. B. With 1.25 mg/ml of bismuth. C. With 5 mg/ml of bismuth. D. With 10 mg/ml of bismuth.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289334&req=5

Fig2: Photographs of incubated phage lysis plates. A. Without bismuth. B. With 1.25 mg/ml of bismuth. C. With 5 mg/ml of bismuth. D. With 10 mg/ml of bismuth.
Mentions: As for the effect of the compounds on the Stx 933 W-encoding phage, a statistically significant decrease (p < 0.05) was observed in the titer of the phage treated with both CBHG and CBCHP in comparison with the untreated titer. The decrease in the titer of the phage was 90% for a CBHG dilution of 1/3, which corresponds to a bismuth concentration of 10 mg/ml; and 86% for a CBHG dilution of 1/6, which is equivalent to a bismuth concentration of 5 mg/ml. In the tests performed with CBCHP, the decrease in the titer of the phage was 82% for a CBCHP dilution of 1/3, which corresponds to a bismuth concentration of 10 mg/ml; and 73% for a CBCHP dilution of 1/6, which is equivalent to a bismuth concentration of 5 mg/ml (Table 3 and Figure 2).Table 3

Bottom Line: Effect on EDL933 virulence factors: significant decrease in active Stx and 933 W Stx phage titer.ELISA did not find significant differences with treatment.The results obtained may be useful in the development of new therapeutic strategies based on the use of CBHG to prevent or improve the prognosis of HUS, as it can be used to control STEC infections.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biochemistry and Pharmaceutical Sciences, Department of Clinical Bacteriology, National University of Rosario, Suipacha 531, S2002LRK Rosario, Santa Fe, Argentina. tomassubils@hotmail.com.

ABSTRACT

Background: Shiga toxin-producing Escherichia coli (STEC) is the causative agent of hemolytic uremic syndrome (HUS). Colloidal bismuth hydroxide gel (CBHG) is an anti-diarrheal and antisecretory compound, which does not inhibit gastrointestinal motility and reaches an in vivo gut concentration of 10.8 mg/ml of bismuth. Its action on bacteria has not been studied. We analyzed its inhibitory effects on STEC, as well as the deactivation of the Shiga toxin (Stx) and its ability to block the spread of genes encoding Stx. We determined a minimum inhibitory concentration and bactericidal concentration for the STEC O157:H7 strain (EDL933), with CBHG and Chobet® bismuth cream with pectin (CBCHP). We analyzed its effect on Stx by means of cytotoxicity assay and ELISA, as well as its effect on the free 933 W Stx phage.

Results: Effect on the EDL933 strain: CBHG: MIC 10 mg/ml of bismuth.

Cbchp: MIC 6 mg/ml and MBC 15 mg/ml of bismuth. Effect on EDL933 virulence factors: significant decrease in active Stx and 933 W Stx phage titer. ELISA did not find significant differences with treatment.

Conclusions: The results obtained may be useful in the development of new therapeutic strategies based on the use of CBHG to prevent or improve the prognosis of HUS, as it can be used to control STEC infections.

Show MeSH
Related in: MedlinePlus