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Antinociceptive and cytotoxic activities of an epiphytic medicinal orchid: Vanda tessellata Roxb.

Chowdhury MA, Rahman MM, Chowdhury MR, Uddin MJ, Sayeed MA, Hossain MA - BMC Complement Altern Med (2014)

Bottom Line: The extracts, at both doses, exhibited a significant (p < 0.05 - < 0.01) dose-dependent antinociceptive activity in hot plate and tail immersion test.The leaf extract has potential antinociceptive activity with minimum cytotoxicity.The present study supports the use of V. tessellata in different inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, 154/A, College Road, Chittagong 4203, Bangladesh. masud@pharm.iiuc.ac.bd.

ABSTRACT

Background: Vanda tessellata (Orchidaceae) has been used in different sorts of ailments such as inflammations, rheumatism, dysentery, bronchitis, dyspepsia and fever in folk medicine. In this study we evaluated the antinociceptive and cytotoxic effect of methanol and aqueous extracts of V. tessellata leaf.

Methods: Oral administration of V. tessellata aqueous (VTA) and methanol (VTM) leaf extracts at 200 and 400 mg/kg body weight (bw) doses were assessed for antinociceptive activity in acetic acid-induced writhing test, hot plate test, and tail immersion test in mice. In this study we also screened for cytotoxicity of the extracts by the brine shrimp (Artemia salina) lethality assay.

Results: The extracts, at both doses, exhibited a significant (p < 0.05 - < 0.01) dose-dependent antinociceptive activity in hot plate and tail immersion test. The reaction time was increased to the thermal stimuli at 200 and 400 mg/kg doses up to 90 min. In acetic acid-induced writhing test, oral administration of VTA and VTM (200 and 400 mg/kg) also decreased the writhing significantly while compared to control. The dose 400 mg/kg showed maximum percentage of pain inhibition 42.37% and 45.08% for VTA and VTM respectively. Diclofenac sodium (10 mg/kg) and nalbuphine (10 mg/kg) were used as reference antinociceptive drugs. Very low cytotoxicity was observed on brine shrimps lethality assay.

Conclusions: The leaf extract has potential antinociceptive activity with minimum cytotoxicity. The present study supports the use of V. tessellata in different inflammatory disorders.

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Related in: MedlinePlus

Effects of various concentrations ofV. tessellataleaves on the viability of brine shrimp nauplii after 24 hrs incubation. VTA = V. tessellata aqueous extract, VTM = V. tessellata methanol extract.
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Fig1: Effects of various concentrations ofV. tessellataleaves on the viability of brine shrimp nauplii after 24 hrs incubation. VTA = V. tessellata aqueous extract, VTM = V. tessellata methanol extract.

Mentions: Cytotoxic effect of the extract is summarized in the Figure 1. The LC50 for methanol and aqueous extract of V. tessellata leaf were found to be 574.32 and 430.41 μg/mL respectively, and that of vincristine sulphate was 0.74 μg/ml.Figure 1


Antinociceptive and cytotoxic activities of an epiphytic medicinal orchid: Vanda tessellata Roxb.

Chowdhury MA, Rahman MM, Chowdhury MR, Uddin MJ, Sayeed MA, Hossain MA - BMC Complement Altern Med (2014)

Effects of various concentrations ofV. tessellataleaves on the viability of brine shrimp nauplii after 24 hrs incubation. VTA = V. tessellata aqueous extract, VTM = V. tessellata methanol extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289330&req=5

Fig1: Effects of various concentrations ofV. tessellataleaves on the viability of brine shrimp nauplii after 24 hrs incubation. VTA = V. tessellata aqueous extract, VTM = V. tessellata methanol extract.
Mentions: Cytotoxic effect of the extract is summarized in the Figure 1. The LC50 for methanol and aqueous extract of V. tessellata leaf were found to be 574.32 and 430.41 μg/mL respectively, and that of vincristine sulphate was 0.74 μg/ml.Figure 1

Bottom Line: The extracts, at both doses, exhibited a significant (p < 0.05 - < 0.01) dose-dependent antinociceptive activity in hot plate and tail immersion test.The leaf extract has potential antinociceptive activity with minimum cytotoxicity.The present study supports the use of V. tessellata in different inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, 154/A, College Road, Chittagong 4203, Bangladesh. masud@pharm.iiuc.ac.bd.

ABSTRACT

Background: Vanda tessellata (Orchidaceae) has been used in different sorts of ailments such as inflammations, rheumatism, dysentery, bronchitis, dyspepsia and fever in folk medicine. In this study we evaluated the antinociceptive and cytotoxic effect of methanol and aqueous extracts of V. tessellata leaf.

Methods: Oral administration of V. tessellata aqueous (VTA) and methanol (VTM) leaf extracts at 200 and 400 mg/kg body weight (bw) doses were assessed for antinociceptive activity in acetic acid-induced writhing test, hot plate test, and tail immersion test in mice. In this study we also screened for cytotoxicity of the extracts by the brine shrimp (Artemia salina) lethality assay.

Results: The extracts, at both doses, exhibited a significant (p < 0.05 - < 0.01) dose-dependent antinociceptive activity in hot plate and tail immersion test. The reaction time was increased to the thermal stimuli at 200 and 400 mg/kg doses up to 90 min. In acetic acid-induced writhing test, oral administration of VTA and VTM (200 and 400 mg/kg) also decreased the writhing significantly while compared to control. The dose 400 mg/kg showed maximum percentage of pain inhibition 42.37% and 45.08% for VTA and VTM respectively. Diclofenac sodium (10 mg/kg) and nalbuphine (10 mg/kg) were used as reference antinociceptive drugs. Very low cytotoxicity was observed on brine shrimps lethality assay.

Conclusions: The leaf extract has potential antinociceptive activity with minimum cytotoxicity. The present study supports the use of V. tessellata in different inflammatory disorders.

Show MeSH
Related in: MedlinePlus